LAMP-1 (hamster) | Developmental Studies Hybridoma Bank UH1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

LAMP-1 (hamster)

catalog :

UH1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

UH1

reactivity :

Chinese hamster

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Monturiol Gross L, Flores Díaz M, Campos Rodríguez D, Mora R, Rodríguez Vega M, Marks D, et al. Internalization of Clostridium perfringens ?-toxin leads to ERK activation and is involved on its cytotoxic effect. Cell Microbiol. 2014;16:535-47 pubmed publisher
Ninio S, Celli J, Roy C. A Legionella pneumophila effector protein encoded in a region of genomic plasticity binds to Dot/Icm-modified vacuoles. PLoS Pathog. 2009;5:e1000278 pubmed publisher
de Felipe K, Glover R, Charpentier X, Anderson O, Reyes M, Pericone C, et al. Legionella eukaryotic-like type IV substrates interfere with organelle trafficking. PLoS Pathog. 2008;4:e1000117 pubmed publisher
Okiyoneda T, Harada K, Takeya M, Yamahira K, Wada I, Shuto T, et al. Delta F508 CFTR pool in the endoplasmic reticulum is increased by calnexin overexpression. Mol Biol Cell. 2004;15:563-74 pubmed
Chakrabarti S, Kobayashi K, Flavell R, Marks C, Miyake K, Liston D, et al. Impaired membrane resealing and autoimmune myositis in synaptotagmin VII-deficient mice. J Cell Biol. 2003;162:543-9 pubmed
Booth J, Kim M, Jankowski A, Schreiber A, Grinstein S. Contrasting requirements for ubiquitylation during Fc receptor-mediated endocytosis and phagocytosis. EMBO J. 2002;21:251-8 pubmed
Caler E, Chakrabarti S, Fowler K, Rao S, Andrews N. The Exocytosis-regulatory protein synaptotagmin VII mediates cell invasion by Trypanosoma cruzi. J Exp Med. 2001;193:1097-104 pubmed
Kima P, Burleigh B, Andrews N. Surface-targeted lysosomal membrane glycoprotein-1 (Lamp-1) enhances lysosome exocytosis and cell invasion by Trypanosoma cruzi. Cell Microbiol. 2000;2:477-86 pubmed
Kundra R, Kornfeld S. Asparagine-linked oligosaccharides protect Lamp-1 and Lamp-2 from intracellular proteolysis. J Biol Chem. 1999;274:31039-46 pubmed
Uthayakumar S, Granger B. Cell surface accumulation of overexpressed hamster lysosomal membrane glycoproteins. Cell Mol Biol Res. 1995;41:405-20 pubmed

product information

Internal ID :

3670

Name :

UH1

Depositor Name :

Granger, B.L. / Uthayakumar, S.

Depositor Institution :

Montana State University

Date Deposited :

1/9/97

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

LAMP-1 (hamster)

Antigen Species :

Hamster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Hamster

Species Tested (legacy) :

hamster, not mouse, rat, or monkey

Initial Publication Pubmed ID :

8867788

Depositor Notes (Special Instructions) :

UH1 recognizes native, methanol-fixed, and PLP-fixed hamster antigen. Doesn't recognize rat, mouse or monkey antigens. UH1 Labels presumptive lysosomes (and late endosomes). For best immunochemistry results, fix cells with methanol or with (para)formaldehyde-lysine-periodate formulation; for the latter, permeabilize with saponin, not with Triton X-100 or NP-40. Unfixed cells that have been permeabilized with saponin can also be stained. For immunoprecipitation, the antigen solubilized with Triton X-100 will bind to GL2A7 coupled to a matrix.

Collections :

CD antigens,Cell signaling

Search Keywords :

Granger, B.L. / Uthayakumar, S., LAMP-1 (hamster), Hamster, MIgG1, Hamster, LAMP1, CD107a, LGP-A, AB_528125, monoclonal, Cell signaling/Cluster Determinant antigens, Immunoprecipitation/Western Blot/Immunofluorescence

Antigen Molecular Weight :

Predicted: 42 kDa; Apparent: 120 kDa

Gene :

LAMP1

Alternate Gene Name(s) :

CD107a, LGP-A

Uniprot ID :

P49129

Antibody Registry ID :

AB_528125

Synonyms (Alternate Clone Names) :

CD107a

Immunogen :

Membrane glycoproteins pre-extracted with saponin from Chinese hamster ovary (CHO) cells and triton-X100-solubilized. Glycoproteins were affinity-purified by binding to wheat-germ agglutinin. The fractions eluting with N-acetyl D-glucosamine were used.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3U1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Lumenal region

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunofluorescence Pubmed IDs :

8867788 12925704 11207602 11342594 11823418 24245664 14595111 18670632 19165328

Western Blot Pubmed IDs :

10521503

Immunoprecipitation Pubmed IDs :

8867788

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell. & Mol. Biol. Res. 41(5), 405-420.

more info or order :

Developmental Studies Hybridoma Bank product webpage