Ultrabithorax and abdominal-A proteins (all isoforms) | Developmental Studies Hybridoma Bank UBX/ABD-A FP6.87 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Ultrabithorax and abdominal-A proteins (all isoforms)

catalog :

UBX/ABD-A FP6.87

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Ubx/ABD-A FP6.87

reactivity :

fruit fly

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Reference
Zhu J, Ordway A, Weber L, Buddika K, Kumar J. Polycomb group (PcG) proteins and Pax6 cooperate to inhibit in vivo reprogramming of the developing Drosophila eye. Development. 2018;145: pubmed publisher
Khila A, Abouheif E, Rowe L. Comparative functional analyses of ultrabithorax reveal multiple steps and paths to diversification of legs in the adaptive radiation of semi-aquatic insects. Evolution. 2014;68:2159-70 pubmed publisher
Lim Y, Yamasaki Y, Tsuda L. Ebi alleviates excessive growth signaling through multiple epigenetic functions in Drosophila. Genes Cells. 2013;18:909-20 pubmed publisher
Liubicich D, Serano J, Pavlopoulos A, Kontarakis Z, Protas M, Kwan E, et al. Knockdown of Parhyale Ultrabithorax recapitulates evolutionary changes in crustacean appendage morphology. Proc Natl Acad Sci U S A. 2009;106:13892-6 pubmed publisher
Khila A, Abouheif E, Rowe L. Evolution of a novel appendage ground plan in water striders is driven by changes in the Hox gene Ultrabithorax. PLoS Genet. 2009;5:e1000583 pubmed publisher
Braendle C, Miura T, Bickel R, Shingleton A, Kambhampati S, Stern D. Developmental origin and evolution of bacteriocytes in the aphid-Buchnera symbiosis. PLoS Biol. 2003;1:E21 pubmed
Grenier J, Carroll S. Functional evolution of the Ultrabithorax protein. Proc Natl Acad Sci U S A. 2000;97:704-9 pubmed
Zheng Z, Khoo A, Fambrough D, Garza L, Booker R. Homeotic gene expression in the wild-type and a homeotic mutant of the moth Manduca sexta. Dev Genes Evol. 1999;209:460-72 pubmed
Stern D. A role of Ultrabithorax in morphological differences between Drosophila species. Nature. 1998;396:463-6 pubmed
Damen W, Hausdorf M, Seyfarth E, Tautz D. A conserved mode of head segmentation in arthropods revealed by the expression pattern of Hox genes in a spider. Proc Natl Acad Sci U S A. 1998;95:10665-70 pubmed
Averof M, Patel N. Crustacean appendage evolution associated with changes in Hox gene expression. Nature. 1997;388:682-6 pubmed
Grenier J, Garber T, Warren R, Whitington P, Carroll S. Evolution of the entire arthropod Hox gene set predated the origin and radiation of the onychophoran/arthropod clade. Curr Biol. 1997;7:547-53 pubmed
Kelsh R, Weinzierl R, White R, Akam M. Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins. Dev Genet. 1994;15:19-31 pubmed

product information

Internal ID :

6593

Name :

Ubx/ABD-A FP6.87

Depositor Name :

White, R.

Depositor Institution :

University of Cambridge

Date Deposited :

5/25/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Ultrabithorax and abdominal-A proteins (all isoforms)

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Crustacean,Drosophila,Drosophila virilis,Gerridae,Grasshopper,Limnoporus,Manduca,Metrobates,Onychophora,Rhagovelia,Spider

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

7514518

Depositor Notes (Special Instructions) :

Broad species specificity

Collections :

Cell adhesion,Cytoskeleton,Drosophila antigens,Nucleus,Transcription factors

Search Keywords :

White, R., Ultrabithorax and abdominal-A proteins (all isoforms), Drosophila, MIgG1, Drosophila/Grasshopper/Drosophila virilis/Onychophora/Limnoporus/Metrobates/Rhagovelia/Spider/Gerridae/Crustacean/Manduca, Ubx, CG10388, bx, Cbx, pbx, abx, bxd, DmUbx, AB_10660834, monoclonal, Drosophila antigens/Cytoskeletal elements/cell adhesion/Transcription factors/Nucleus, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms, from 35 to 40kDa

Gene :

Ubx/abd-A

Alternate Gene Name(s) :

CG10388, bx, Cbx, pbx, abx, bxd, DmUbx

Uniprot ID :

P83949 P29555

Antibody Registry ID :

AB_10660834

Synonyms (Alternate Clone Names) :

FP6.87, anti-UbdA

Immunogen :

Recombinant Drosophila Ultrabithorax lacking the first 26 aa from N-terminus fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

NSO

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 350-363 (Uniprot# P83949-1) region contains at least part of the epitope.

Epitope Map PubMed ID :

7514518

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

7514518 9262403 24766229 10415323 9724761 19649305

Immunofluorescence Pubmed IDs :

7514518 9262403 10639143 9853753 9259556 14551917 23919509 19666517 29530880 30562515

Additional Information :

This antibody cross-reacts with abdominal-A and recognizes all isoforms of the two proteins. Based on sequence homology, this antibody should also work in Aedes, Bombyx, Apis, and Tribolium [PMID 7514518].

DSHB Growth Medium :

RPMI

References (legacy) :

Dev. Gen. 15, 19-31.

more info or order :

Developmental Studies Hybridoma Bank product webpage