product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
CD11a (Integrin alpha-L, LFA-1 alpha subunit), human
catalog :
TS1/22.1.1.13.3
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
TS1/22.1.1.13.3
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
citations: 17
Reference |
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Godwin J, d Apice A, Cowan P. Characterization of pig intercellular adhesion molecule-2 and its interaction with human LFA-1. Am J Transplant. 2004;4:515-25 pubmed
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Spaargaren M, Beuling E, Rurup M, Meijer H, Klok M, Middendorp S, et al. The B cell antigen receptor controls integrin activity through Btk and PLCgamma2. J Exp Med. 2003;198:1539-50 pubmed
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Beals C, Edwards A, Gottschalk R, Kuijpers T, Staunton D. CD18 activation epitopes induced by leukocyte activation. J Immunol. 2001;167:6113-22 pubmed
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Lu C, Ferzly M, Takagi J, Springer T. Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation. J Immunol. 2001;166:5629-37 pubmed
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Huang C, Springer T. A binding interface on the I domain of lymphocyte function-associated antigen-1 (LFA-1) required for specific interaction with intercellular adhesion molecule 1 (ICAM-1). J Biol Chem. 1995;270:19008-16 pubmed
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Petruzzelli L, Maduzia L, Springer T. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol. 1995;155:854-66 pubmed
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Sanchez Madrid F, Krensky A, Ware C, Robbins E, Strominger J, Burakoff S, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci U S A. 1982;79:7489-93 pubmed
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Sanchez Madrid F, Nagy J, Robbins E, Simon P, Springer T. A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule. J Exp Med. 1983;158:1785-1803 pubmed
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Fischer A, Durandy A, Sterkers G, Griscelli C. Role of the LFA-1 molecule in cellular interactions required for antibody production in humans. J Immunol. 1986;136:3198-203 pubmed
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Van Epps D, Potter J, Vachula M, Smith C, Anderson D. Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody. J Immunol. 1989;143:3207-10 pubmed
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Dustin M, Springer T. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature. 1989;341:619-24 pubmed
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Basson C, Knowles W, Bell L, Albelda S, Castronovo V, Liotta L, et al. Spatiotemporal segregation of endothelial cell integrin and nonintegrin extracellular matrix-binding proteins during adhesion events. J Cell Biol. 1990;110:789-801 pubmed
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Takahashi S, Doss C, Levy S, Levy R. TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen. J Immunol. 1990;145:2207-13 pubmed
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Denning S, Le P, Singer K, Haynes B. Antibodies against the CD44 p80, lymphocyte homing receptor molecule augment human peripheral blood T cell activation. J Immunol. 1990;144:7-15 pubmed
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Yellin M, Lee J, Chess L, Lederman S. A human CD4- T cell leukemia subclone with contact-dependent helper function. J Immunol. 1991;147:3389-95 pubmed
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Beekhuizen H, Corsèl van Tilburg A, Blokland I, Van Furth R. Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells. Immunology. 1991;74:661-9 pubmed
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Ardman B, Sikorski M, Staunton D. CD43 interferes with T-lymphocyte adhesion. Proc Natl Acad Sci U S A. 1992;89:5001-5 pubmed
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product information
Internal ID :
2320
Name :
TS1/22.1.1.13.3
Depositor Name :
Springer, T.A.
Depositor Institution :
Center for Blood Research
Date Deposited :
4/4/89
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
CD11a (Integrin alpha-L, LFA-1 alpha subunit), human
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Initial Publication Pubmed ID :
6984191
Depositor Notes (Special Instructions) :
Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting. Binds to all leukocytes.
Collections :
CD antigens,Cell adhesion,Cell markers,Cell signaling,Human
Search Keywords :
Springer, T.A., CD11a (Integrin alpha-L, LFA-1 alpha subunit), human, Human, MIgG1, Human, ITGAL, LFA-1, AB_528132, monoclonal, Human/Cell signaling/Cluster Determinant antigens/Cell adhesion/Cell markers, Immunohistochemistry/Immunofluorescence/Function Blocking/Immunoprecipitation/FACS
Antigen Molecular Weight :
Predicted: 129 kDa; Apparent: 180 kDa
Gene :
ITGAL
Alternate Gene Name(s) :
LFA-1
Uniprot ID :
P20701
Antibody Registry ID :
AB_528132
Synonyms (Alternate Clone Names) :
TS1/22
Immunogen :
Human HLA-DRCTL line cytolytic T-lymphocytes.
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3X63Ag8.6.5.3
Epitope Mapped :
Yes
Epitope Location or Sequence :
I domain (aa 248-303)
Epitope Map PubMed ID :
7642561
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation
Immunoblotting (legacy) :
No
Immunofluorescence Pubmed IDs :
3514755 11714770
Immunoprecipitation Pubmed IDs :
6984191 7608563 6196430
Function Blocking Pubmed IDs :
6984191 2477710 1723715 15023143 3514755 1967267 2681413 2398277 1594606 7608563 11714770
FACS Pubmed IDs :
2681413 11313403 2477710 7608563 1834738
Pubmed IDs :
2407741 14610042
DSHB Growth Medium :
RPMI
References (legacy) :
PNAS 79, 7489-7493.
more info or order :
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