CD18 (integrin beta-2; beta subunit of CD11a, b, c), human | Developmental Studies Hybridoma Bank TS1/18.1.2.11.4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

CD18 (integrin beta-2; beta subunit of CD11a, b, c), human

catalog :

TS1/18.1.2.11.4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TS1/18.1.2.11.4

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 15

Reference
Burns S, Hardy S, Buddle J, Yong K, Jones G, Thrasher A. Maturation of DC is associated with changes in motile characteristics and adherence. Cell Motil Cytoskeleton. 2004;57:118-32 pubmed
Beals C, Edwards A, Gottschalk R, Kuijpers T, Staunton D. CD18 activation epitopes induced by leukocyte activation. J Immunol. 2001;167:6113-22 pubmed
Lu C, Ferzly M, Takagi J, Springer T. Epitope mapping of antibodies to the C-terminal region of the integrin beta 2 subunit reveals regions that become exposed upon receptor activation. J Immunol. 2001;166:5629-37 pubmed
Cywes C, Godenir N, Hoppe H, Scholle R, Steyn L, Kirsch R, et al. Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells. Infect Immun. 1996;64:5373-83 pubmed
Petruzzelli L, Maduzia L, Springer T. Activation of lymphocyte function-associated molecule-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) mimicked by an antibody directed against CD18. J Immunol. 1995;155:854-66 pubmed
McGuire S, Bajt M. Distinct ligand binding sites in the I domain of integrin alpha M beta 2 that differentially affect a divalent cation-dependent conformation. J Biol Chem. 1995;270:25866-71 pubmed
Sanchez Madrid F, Krensky A, Ware C, Robbins E, Strominger J, Burakoff S, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci U S A. 1982;79:7489-93 pubmed
Sanchez Madrid F, Nagy J, Robbins E, Simon P, Springer T. A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule. J Exp Med. 1983;158:1785-1803 pubmed
Fischer A, Durandy A, Sterkers G, Griscelli C. Role of the LFA-1 molecule in cellular interactions required for antibody production in humans. J Immunol. 1986;136:3198-203 pubmed
Van Epps D, Potter J, Vachula M, Smith C, Anderson D. Suppression of human lymphocyte chemotaxis and transendothelial migration by anti-LFA-1 antibody. J Immunol. 1989;143:3207-10 pubmed
Dustin M, Springer T. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature. 1989;341:619-24 pubmed
Takahashi S, Doss C, Levy S, Levy R. TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen. J Immunol. 1990;145:2207-13 pubmed
Yellin M, Lee J, Chess L, Lederman S. A human CD4- T cell leukemia subclone with contact-dependent helper function. J Immunol. 1991;147:3389-95 pubmed
Beekhuizen H, Corsèl van Tilburg A, Blokland I, Van Furth R. Characterization of the adherence of human monocytes to cytokine-stimulated human macrovascular endothelial cells. Immunology. 1991;74:661-9 pubmed
Ardman B, Sikorski M, Staunton D. CD43 interferes with T-lymphocyte adhesion. Proc Natl Acad Sci U S A. 1992;89:5001-5 pubmed

product information

Internal ID :

2314

Name :

TS1/18.1.2.11.4

Depositor Name :

Springer, T.A.

Depositor Institution :

Center for Blood Research

Date Deposited :

4/4/89

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

CD18 (integrin beta-2; beta subunit of CD11a, b, c), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

does not cross react with mouse

Initial Publication Pubmed ID :

6984191

Depositor Notes (Special Instructions) :

Does not cross react with mouse. Binds to all leukocytes. Blocks CTL killing, ADCC killing of NK & granulocytes, homotypic B cell adhesion, gammaINF adhesion of monocytes, leukocyte-epithelial cell interactions. Doesn't appear to work for western blotting.

Collections :

CD antigens,Cell adhesion,Cell markers,Cell signaling,Human

Search Keywords :

Springer, T.A., CD18 (integrin beta-2; beta subunit of CD11a, b, c), human, Human, MIgG1, Human, ITGB2, CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1, AB_2128634, monoclonal, Human/Cell signaling/Cluster Determinant antigens/Cell adhesion/Cell markers, Immunofluorescence/Immunohistochemistry/Immunofluorescence/Function Blocking/Immunoprecipitation/FACS

Antigen Molecular Weight :

Predicted: 85 kDa; Apparent: 93 kDa

Gene :

ITGB2

Alternate Gene Name(s) :

CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1

Uniprot ID :

P05107

Antibody Registry ID :

AB_2128634

Synonyms (Alternate Clone Names) :

TS1/18

Immunogen :

Human HLA-DRCTL line cytolytic T-lymphocytes.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.6.5.3

Epitope Mapped :

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

3514755 11714770

Immunoprecipitation Pubmed IDs :

6984191 8945590 7592772 7608563 6196430

Function Blocking Pubmed IDs :

6984191 8945590 14691951 2477710 3514755 2681413 2398277 1594606 11714770

FACS Pubmed IDs :

6984191 11313403 2681413 7608563 6196430 1834738 11714770

Pubmed IDs :

1723715

Additional Information :

CD18 is a general marker of leukocytes.

DSHB Growth Medium :

RPMI

References (legacy) :

PNAS 79, 7489-7493.

more info or order :

Developmental Studies Hybridoma Bank product webpage