alkaline phosphatase, tissue non-specific isoenzyme | Developmental Studies Hybridoma Bank TRA-2-54/2J product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

alkaline phosphatase, tissue non-specific isoenzyme

catalog :

TRA-2-54/2J

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TRA-2-54/2J

reactivity :

human, Rhesus monkey

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 3

Reference
Macdonald D, Tessari M, Boogaard I, Smith M, Pulli K, Szynol A, et al. Quantification assays for total and polyglutamine-expanded huntingtin proteins. PLoS ONE. 2014;9:e96854 pubmed publisher
Andrews P, Meyer L, Bednarz K, Harris H. Two monoclonal antibodies recognizing determinants on human embryonal carcinoma cells react specifically with the liver isozyme of human alkaline phosphatase. Hybridoma. 1984;3:33-9 pubmed
Swallow D, Povey S, Parkar M, Andrews P, Harris H, Pym B, et al. Mapping of the gene coding for the human liver/bone/kidney isozyme of alkaline phosphatase to chromosome 1. Ann Hum Genet. 1986;50:229-35 pubmed

product information

Internal ID :

329

Name :

TRA-2-54/2J

Depositor Name :

Andrews, P.W.

Depositor Institution :

University of Sheffield

Depositor Website :

https://www.shef.ac.uk/bms/research/andrews

Date Deposited :

12/11/90

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

alkaline phosphatase, tissue non-specific isoenzyme

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Baboon,Human,Mandrill,Non-human primates,Rhesus monkey

Species Tested (legacy) :

human, apes, old world monkeys, other mammals

Initial Publication Pubmed ID :

6205984

Depositor Notes (Special Instructions) :

This antibody recognizes alkaline phosphatase isozyme from liver, bone and kidney but does not recognize intestinal or placental alkaline phosphatases.

Collections :

Enzymes,Human,Stem cell

Search Keywords :

Andrews, P.W., alkaline phosphatase, tissue non-specific isoenzyme , Human, MIgG1, Human/Non-human primates/baboon/rhesus monkey/mandrill, ALPL, HOPS, TNSALP, , Monoclonal , Human/Enzymes, Immunohistochemistry/Immunofluorescence/western blot/Immunoprecipitation/ELISA/FACS

Antigen Molecular Weight :

~80 kDa

Gene :

ALPL

Alternate Gene Name(s) :

HOPS, TNSALP

Uniprot ID :

P05186

Antibody Registry ID :

AB_528074

Synonyms (Alternate Clone Names) :

chromosome 1, 80 kDa polypeptide

Immunogen :

Embryonal carcinoma (EC) 2102Ep cells

Immunogen Sequence :

Expanded polyglutamine domain (polyQ)

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8-653

Epitope Mapped :

Recommended Applications :

ELISA,FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunofluorescence Pubmed IDs :

6205984

Western Blot Pubmed IDs :

6205984 24816435

Immunoprecipitation Pubmed IDs :

6205984 3446011

ELISA Pubmed IDs :

6205984

FACS Pubmed IDs :

6205984

Additional Information :

This antibody does not react with mouse ALP. RRID:AB_528074

DSHB Growth Medium :

Iscove's

References (legacy) :

Hybridoma 3, 33-39.; Ann. Human Genet. 50, 229-235.; Genesis 52, 49-55.

more info or order :

Developmental Studies Hybridoma Bank product webpage