alkaline phosphatase, tissue non-specific isoenzyme | Developmental Studies Hybridoma Bank TRA-2-49/6E product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

alkaline phosphatase, tissue non-specific isoenzyme

catalog :

TRA-2-49/6E

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TRA-2-49/6E

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; scFv; 1:100; fig 1 B-i	Farup J, De Lisio M, Rahbek S, Bjerre J, Vendelbo M, Boppart M, et al. Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle. J Appl Physiol (1985). 2015;119:1053-63 pubmed publisher
	Sun Y, Paşca S, Portmann T, Goold C, Worringer K, Guan W, et al. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients. elife. 2016;5: pubmed publisher
	Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131:861-72 pubmed
	Andrews P, Meyer L, Bednarz K, Harris H. Two monoclonal antibodies recognizing determinants on human embryonal carcinoma cells react specifically with the liver isozyme of human alkaline phosphatase. Hybridoma. 1984;3:33-9 pubmed

product information

Internal ID :

324

Name :

TRA-2-49/6E

Depositor Name :

Andrews, P.W.

Depositor Institution :

University of Sheffield

Depositor Website :

https://www.shef.ac.uk/bms/research/andrews

Date Deposited :

12/11/90

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

alkaline phosphatase, tissue non-specific isoenzyme

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Marmoset,Non-human primates,Squirrel monkey

Species Tested (legacy) :

human, apes, old/new world monkeys

Initial Publication Pubmed ID :

6205984

Depositor Notes (Special Instructions) :

This antibody recognizes alkaline phosphatase isozyme from liver, bone and kidney but does not recognize intestinal or placental alkaline phosphatases.

Collections :

Enzymes,Human,Stem cell

Search Keywords :

Andrews, P.W., alkaline phosphatase, tissue non-specific isoenzyme , Human, MIgG1, Human/Non-human primates/Marmoset/Squirrel Monkey, ALPL, HOPS, TNSALP, AB_528073, Monoclonal , Human/Enzymes, Immunohistochemistry/Immunofluorescence/western blot/Immunoprecipitation/ELISA/FACS

Antigen Molecular Weight :

~80 kDa

Gene :

ALPL

Alternate Gene Name(s) :

HOPS, TNSALP

Uniprot ID :

P05186

Antibody Registry ID :

AB_528073

Synonyms (Alternate Clone Names) :

chromosome 1, 80 kDa polypeptide

Immunogen :

Embryonal carcinoma (EC) 2102Ep cells

Immunogen Sequence :

Full length sequence

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8-653

Epitope Mapped :

Recommended Applications :

ELISA,FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunofluorescence Pubmed IDs :

6205984 18035408 26404620 27458797

Western Blot Pubmed IDs :

6205984

Immunoprecipitation Pubmed IDs :

6205984

ELISA Pubmed IDs :

6205984

FACS Pubmed IDs :

6205984

Additional Information :

RRID:AB_528073; This antibody does not react with mouse ALP.

DSHB Growth Medium :

Iscove's

References (legacy) :

Hybridoma 3, 33-39.; Ann. Human Genet. 50, 229-235.

more info or order :

Developmental Studies Hybridoma Bank product webpage