Hexon protein, human adenovirus 5 | Developmental Studies Hybridoma Bank TC31-9C12.C9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Hexon protein, human adenovirus 5

catalog :

TC31-9C12.C9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TC31-9C12.C9

reactivity :

Human adenovirus C

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 17

Reference
Kaulfuß M, Wensing I, Windmann S, Hrycak C, Bayer W. Induction of complex immune responses and strong protection against retrovirus challenge by adenovirus-based immunization depends on the order of vaccine delivery. Retrovirology. 2017;14:8 pubmed publisher
Bottermann M, Lode H, Watkinson R, Foss S, Sandlie I, Andersen J, et al. Antibody-antigen kinetics constrain intracellular humoral immunity. Sci Rep. 2016;6:37457 pubmed publisher
Moyer C, Besser E, Nemerow G. A Single Maturation Cleavage Site in Adenovirus Impacts Cell Entry and Capsid Assembly. J Virol. 2016;90:521-32 pubmed publisher
Watkinson R, McEwan W, Tam J, Vaysburd M, James L. TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus. PLoS Pathog. 2015;11:e1005253 pubmed publisher
Luisoni S, Suomalainen M, Boucke K, Tanner L, Wenk M, Guan X, et al. Co-option of Membrane Wounding Enables Virus Penetration into Cells. Cell Host Microbe. 2015;18:75-85 pubmed publisher
Fletcher A, Mallery D, Watkinson R, Dickson C, James L. Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21. Proc Natl Acad Sci U S A. 2015;112:10014-9 pubmed publisher
Wang I, Suomalainen M, Andriasyan V, Kilcher S, Mercer J, Neef A, et al. Tracking viral genomes in host cells at single-molecule resolution. Cell Host Microbe. 2013;14:468-80 pubmed publisher
Suomalainen M, Luisoni S, Boucke K, Bianchi S, Engel D, Greber U. A direct and versatile assay measuring membrane penetration of adenovirus in single cells. J Virol. 2013;87:12367-79 pubmed publisher
Myers N, Skorohodova K, Gounder A, Smith J. Directed evolution of mutator adenoviruses resistant to antibody neutralization. J Virol. 2013;87:6047-50 pubmed publisher
Hauler F, Mallery D, McEwan W, Bidgood S, James L. AAA ATPase p97/VCP is essential for TRIM21-mediated virus neutralization. Proc Natl Acad Sci U S A. 2012;109:19733-8 pubmed publisher
McEwan W, Hauler F, Williams C, Bidgood S, Mallery D, Crowther R, et al. Regulation of virus neutralization and the persistent fraction by TRIM21. J Virol. 2012;86:8482-91 pubmed publisher
Moyer C, Wiethoff C, Maier O, Smith J, Nemerow G. Functional genetic and biophysical analyses of membrane disruption by human adenovirus. J Virol. 2011;85:2631-41 pubmed publisher
Nguyen E, Nemerow G, Smith J. Direct evidence from single-cell analysis that human {alpha}-defensins block adenovirus uncoating to neutralize infection. J Virol. 2010;84:4041-9 pubmed publisher
Smith J, Cassany A, Gerace L, Ralston R, Nemerow G. Neutralizing antibody blocks adenovirus infection by arresting microtubule-dependent cytoplasmic transport. J Virol. 2008;82:6492-500 pubmed publisher
Smith J, Nemerow G. Mechanism of adenovirus neutralization by Human alpha-defensins. Cell Host Microbe. 2008;3:11-9 pubmed publisher
Saban S, Nepomuceno R, Gritton L, Nemerow G, Stewart P. CryoEM structure at 9A resolution of an adenovirus vector targeted to hematopoietic cells. J Mol Biol. 2005;349:526-37 pubmed
Varghese R, Mikyas Y, Stewart P, Ralston R. Postentry neutralization of adenovirus type 5 by an antihexon antibody. J Virol. 2004;78:12320-32 pubmed

product information

Internal ID :

5012

Name :

TC31-9C12.C9

Depositor Name :

Fayadat-Dilman, L. / Olijve, W.

Depositor Institution :

Schering Plough Biopharma

Date Deposited :

9/23/08

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Hexon protein, human adenovirus 5

Antigen Species :

human adenovirus C serotype 5

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

human adenovirus 5

Species Tested (legacy) :

viral

Initial Publication Pubmed ID :

15507619

Collections :

Microbe antibodies

Search Keywords :

Fayadat-Dilman, L. / Olijve, W., Hexon protein, human adenovirus 5, human adenovirus C serotype 5, MIgG1, kappa light chain, human adenovirus 5, L3, , AB_1553405, monoclonal, Microbe antibodies, Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/Function Blocking/FACS

Antigen Molecular Weight :

Predicted: 108 kDa; Apparent: 108 kDa (monomer, heat-denatured); > 200kDa (trimer, not heat-denatured)

Gene :

Uniprot ID :

P04133

Antibody Registry ID :

AB_1553405

Synonyms (Alternate Clone Names) :

9C12

Immunogen :

Purified human adenovirus serotype 5

Immunogen Sequence :

full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag

Epitope Mapped :

Yes

Epitope Location or Sequence :

The epitope is most likely formed by residues from the gap of aa 431–436, which includes HVR8 from the FG1 loop, and possibly by residues from the long gap of aa 136–164, which includes most of HVR1. The G434D mutation disrupts binding of the antibody.

Epitope Map PubMed ID :

15890367 23487468

Recommended Applications :

ELISA,FACS,Function Blocking,Immunofluorescence,Immunoprecipitation,Western Blot

Immunofluorescence Pubmed IDs :

15507619 18448546 21209115 20130047 24139403 26159720 26506431 24027314

Western Blot Pubmed IDs :

15507619

Immunoprecipitation Pubmed IDs :

15507619 23487468

ELISA Pubmed IDs :

15507619 26491163

Function Blocking Pubmed IDs :

15507619 18448546 23487468 23091005 22647693 26506431 26150489

FACS Pubmed IDs :

18191790

Pubmed IDs :

27881870

Additional Information :

Hexon is the major capsid protein. The epitope recognized by TC31-9C12.C9 is likely conformation-specific since this mAb can not bind heat denatured hexon. TC31-9C12.C9 immunoprecipitates the complete set of proteins normally associated with intact Ad5 particles; in contrast, TC31-27F11.C2 (a mAb available in the DSHB collection, isolated from the same fusion that generated TC31-9C12.C9) pulls down only free hexon protein. While TC31-9C12.C9 neutralizes Ad5, TC31-27F11.C2 doesn't. TC31-27F11.C2 is the perfect isotype-matched nonneutralizing control Ab for TC31-9C12.C9.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Virol. 78, 12320-12332.; J. Virol. 82, 6492-6500.

more info or order :

Developmental Studies Hybridoma Bank product webpage