Hexon protein, human adenovirus 5 | Developmental Studies Hybridoma Bank TC31-27F11.C2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Hexon protein, human adenovirus 5

catalog :

TC31-27F11.C2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

TC31-27F11.C2

reactivity :

Human adenovirus C

application :

western blot, ELISA, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Reference
Smith J, Cassany A, Gerace L, Ralston R, Nemerow G. Neutralizing antibody blocks adenovirus infection by arresting microtubule-dependent cytoplasmic transport. J Virol. 2008;82:6492-500 pubmed publisher
Varghese R, Mikyas Y, Stewart P, Ralston R. Postentry neutralization of adenovirus type 5 by an antihexon antibody. J Virol. 2004;78:12320-32 pubmed

product information

Internal ID :

5017

Name :

TC31-27F11.C2

Depositor Name :

Fayadat-Dilman, L. / Olijve, W.

Depositor Institution :

Schering Plough Biopharma

Date Deposited :

9/23/08

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Hexon protein, human adenovirus 5

Antigen Species :

human adenovirus C serotype 5

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

human adenovirus 5

Species Tested (legacy) :

viral

Initial Publication Pubmed ID :

15507619

Collections :

Microbe antibodies

Search Keywords :

Fayadat-Dilman, L. / Olijve, W., Hexon protein, human adenovirus 5, human adenovirus C serotype 5, MIgG1, kappa light chain, human adenovirus 5, L3, , AB_1553403, monoclonal, Microbe antibodies, Western Blot/Immunoprecipitation/ELISA

Antigen Molecular Weight :

Predicted: 108 kDa; Apparent: 108 kDa (monomer, heat-denatured); > 200kDa (trimer, not heat-denatured)

Gene :

Uniprot ID :

P04133

Antibody Registry ID :

AB_1553403

Synonyms (Alternate Clone Names) :

27F11

Immunogen :

Purified human adenovirus serotype 5

Immunogen Sequence :

full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag

Epitope Mapped :

Recommended Applications :

ELISA,Immunoprecipitation,Western Blot

Western Blot Pubmed IDs :

15507619

Immunoprecipitation Pubmed IDs :

15507619

ELISA Pubmed IDs :

15507619

Pubmed IDs :

18448546

Additional Information :

Hexon is the major capsid protein. The epitope recognized by TC31-27F11.C2 is likely conformation-specific since this mAb can not bind heat denatured hexon. TC31-27F11.C2 immunoprecipitates only free hexon protein; in contrast, TC31-9C12.C9 (a mAb available in the DSHB collection, isolated from the same fusion that generated TC31-27F11.C2) pulls down the complete set of proteins normally associated with intact Ad5 particles. The TC31-27F11.C2 epitope is likely not accessible on the exposed surface of intact Ad particles. While TC31-9C12.C9 neutralizes Ad5, TC31-27F11.C2 doesn't. TC31-27F11.C2 is the perfect isotype-matched nonneutralizing control Ab for TC31-9C12.C9.

DSHB Growth Medium :

RPMI

more info or order :

Developmental Studies Hybridoma Bank product webpage