Na-K-Cl cotransporter | Developmental Studies Hybridoma Bank T9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Na-K-Cl cotransporter

catalog :

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 4

Reference
Gimenez I, Forbush B. Short-term stimulation of the renal Na-K-Cl cotransporter (NKCC2) by vasopressin involves phosphorylation and membrane translocation of the protein. J Biol Chem. 2003;278:26946-51 pubmed
D Andrea Winslow L, Strohmeier G, Rossi B, Hofman P. Identification of a sea urchin Na(+)/K(+)/2Cl(-) cotransporter (NKCC): microfilament-dependent surface expression is mediated by hypotonic shock and cyclic AMP. J Exp Biol. 2001;204:147-56 pubmed
D ANDREA L, Lytle C, Matthews J, Hofman P, Forbush B, Madara J. Na:K:2Cl cotransporter (NKCC) of intestinal epithelial cells. Surface expression in response to cAMP. J Biol Chem. 1996;271:28969-76 pubmed
Lytle C, Xu J, Biemesderfer D, Forbush B. Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies. Am J Physiol. 1995;269:C1496-505 pubmed

product information

Internal ID :

10955

Name :

Depositor Name :

Lytle, C. / Forbush III, B.

Depositor Institution :

Yale University

Date Deposited :

11/11/14

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Na-K-Cl cotransporter

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Positive Tested Species Reactivity :

Mammal

Initial Publication Pubmed ID :

8572179

Depositor Notes (Special Instructions) :

Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T9 is better than T4 in recognizing NKCC2, particularly by IF (PMID: 8572179, 12732642). T9 recpgmozes the 196kDa cotransporter exclusively on western blots [PMID: 8910547].

Collections :

Cell signaling,Human,Neurodevelopment

Search Keywords :

Lytle/Forbush III, Na-K-Cl cotransporters, mouse, NKCC2

Antigen Molecular Weight :

Predicted: 121.4kDa Apparent: 145-205kDa

Gene :

SLC12A1, SLC12A2

Alternate Gene Name(s) :

NKCC1 and NKCC2

Uniprot ID :

P55011 Q13621

Antibody Registry ID :

AB_2618107

Immunogen :

Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein

Immunogen Sequence :

human NKCC1 C-terminus (Met902-Ser1212)

Clonality :

Monoclonal

Myeloma Strain :

P3x63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

a.a. 902-1212

Recommended Applications :

Immunofluorescence,Western Blot

Immunofluorescence Pubmed IDs :

12732642 8910547

Western Blot Pubmed IDs :

8572179 8910547 11104718

Immunoprecipitation Pubmed IDs :

8572179

Depositor Growth Medium :

RPMI 1640 medium plus 15% FCS plus pen-strep

Additional Information :

RRID:AB_2618107

DSHB Growth Medium :

RPMI

more info or order :

Developmental Studies Hybridoma Bank product webpage