Na-K-Cl cotransporter | Developmental Studies Hybridoma Bank T4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Na-K-Cl cotransporter

catalog :

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 7g immunocytochemistry; mouse; 1:100; fig 7d	Moyon S, Frawley R, Maréchal D, Huang D, Marshall Phelps K, Kegel L, et al. TET1-mediated DNA hydroxymethylation regulates adult remyelination in mice. Nat Commun. 2021;12:3359 pubmed publisher
western blot; mouse; loading ...; fig 6f	Bhandage A, Olivera G, Kanatani S, Thompson E, Lore K, Varas Godoy M, et al. A motogenic GABAergic system of mononuclear phagocytes facilitates dissemination of coccidian parasites. elife. 2020;9: pubmed publisher
western blot; rat; loading ...; fig 5C	Wright R, Newey S, Ilie A, Wefelmeyer W, Raimondo J, Ginham R, et al. Neuronal Chloride Regulation via KCC2 Is Modulated through a GABAB Receptor Protein Complex. J Neurosci. 2017;37:5447-5462 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 7	Limmer F, Schinner E, Castrop H, Vitzthum H, Hofmann F, Schlossmann J. Regulation of the Na(+)-K(+)-2Cl(-) cotransporter by cGMP/cGMP-dependent protein kinase I after furosemide administration. FEBS J. 2015;282:3786-98 pubmed publisher
western blot; rat; 1:500; loading ...; fig s1	Deidda G, Allegra M, Cerri C, Naskar S, Bony G, Zunino G, et al. Early depolarizing GABA controls critical-period plasticity in the rat visual cortex. Nat Neurosci. 2015;18:87-96 pubmed publisher
	Porter D, Moore A, Cobern J, Padmanabhan V, Goodman R, Coolen L, et al. Prenatal Testosterone Exposure Alters GABAergic Synaptic Inputs to GnRH and KNDy Neurons in a Sheep Model of Polycystic Ovarian Syndrome. Endocrinology. 2019;160:2529-2542 pubmed publisher
	Côté M, Gandhi S, Zambrotta M, Houle J. Exercise modulates chloride homeostasis after spinal cord injury. J Neurosci. 2014;34:8976-87 pubmed publisher
	Mejia Gervacio S, Murray K, Lledo P. NKCC1 controls GABAergic signaling and neuroblast migration in the postnatal forebrain. Neural Dev. 2011;6:4 pubmed publisher
	Mykoniatis A, Shen L, Fedor Chaiken M, Tang J, Tang X, Worrell R, et al. Phorbol 12-myristate 13-acetate-induced endocytosis of the Na-K-2Cl cotransporter in MDCK cells is associated with a clathrin-dependent pathway. Am J Physiol Cell Physiol. 2010;298:C85-97 pubmed publisher
	Katoh F, Cozzi R, Marshall W, Goss G. Distinct Na+/K+/2Cl- cotransporter localization in kidneys and gills of two euryhaline species, rainbow trout and killifish. Cell Tissue Res. 2008;334:265-81 pubmed publisher
	Hiroi J, Yasumasu S, McCormick S, Hwang P, Kaneko T. Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish. J Exp Biol. 2008;211:2584-99 pubmed publisher
	Marshall W, Katoh F, Main H, Sers N, Cozzi R. Focal adhesion kinase and beta1 integrin regulation of Na+, K+, 2Cl- cotransporter in osmosensing ion transporting cells of killifish, Fundulus heteroclitus. Comp Biochem Physiol A Mol Integr Physiol. 2008;150:288-300 pubmed publisher
	Burnett K, Bain L, Baldwin W, Callard G, Cohen S, Di Giulio R, et al. Fundulus as the premier teleost model in environmental biology: opportunities for new insights using genomics. Comp Biochem Physiol Part D Genomics Proteomics. 2007;2:257-86 pubmed
	Hiroi J, McCormick S. Variation in salinity tolerance, gill Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush, Salvelinus fontinalis and Salmo salar. J Exp Biol. 2007;210:1015-24 pubmed
	Tipsmark C, Luckenbach J, Madsen S, Borski R. IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass. Am J Physiol Regul Integr Comp Physiol. 2007;292:R535-43 pubmed
	Hiroi J, Miyazaki H, Katoh F, Ohtani Kaneko R, Kaneko T. Chloride turnover and ion-transporting activities of yolk-sac preparations (yolk balls) separated from Mozambique tilapia embryos and incubated in freshwater and seawater. J Exp Biol. 2005;208:3851-8 pubmed
	Hiroi J, McCormick S, Ohtani Kaneko R, Kaneko T. Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase, Na+/K+/2Cl- cotransporter and CFTR anion channel. J Exp Biol. 2005;208:2023-36 pubmed
	Marshall W, Ossum C, Hoffmann E. Hypotonic shock mediation by p38 MAPK, JNK, PKC, FAK, OSR1 and SPAK in osmosensing chloride secreting cells of killifish opercular epithelium. J Exp Biol. 2005;208:1063-77 pubmed
	Tipsmark C, Madsen S, Borski R. Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis). J Exp Zool A Comp Exp Biol. 2004;301:979-91 pubmed
	Tipsmark C, Madsen S, Seidelin M, Christensen A, Cutler C, Cramb G. Dynamics of Na(+),K(+),2Cl(-) cotransporter and Na(+),K(+)-ATPase expression in the branchial epithelium of brown trout (Salmo trutta) and Atlantic salmon (Salmo salar). J Exp Zool. 2002;293:106-18 pubmed
	Marshall W, Lynch E, Cozzi R. Redistribution of immunofluorescence of CFTR anion channel and NKCC cotransporter in chloride cells during adaptation of the killifish Fundulus heteroclitus to sea water. J Exp Biol. 2002;205:1265-73 pubmed
	Alvaro D, Gigliozzi A, Marucci L, Alpini G, Barbaro B, Monterubbianesi R, et al. Corticosteroids modulate the secretory processes of the rat intrahepatic biliary epithelium. Gastroenterology. 2002;122:1058-69 pubmed
	D Andrea Winslow L, Strohmeier G, Rossi B, Hofman P. Identification of a sea urchin Na(+)/K(+)/2Cl(-) cotransporter (NKCC): microfilament-dependent surface expression is mediated by hypotonic shock and cyclic AMP. J Exp Biol. 2001;204:147-56 pubmed
	D ANDREA L, Lytle C, Matthews J, Hofman P, Forbush B, Madara J. Na:K:2Cl cotransporter (NKCC) of intestinal epithelial cells. Surface expression in response to cAMP. J Biol Chem. 1996;271:28969-76 pubmed
	Biemesderfer D, Payne J, Lytle C, Forbush B. Immunocytochemical studies of the Na-K-Cl cotransporter of shark kidney. Am J Physiol. 1996;270:F927-36 pubmed
	Lytle C, Xu J, Biemesderfer D, Forbush B. Distribution and diversity of Na-K-Cl cotransport proteins: a study with monoclonal antibodies. Am J Physiol. 1995;269:C1496-505 pubmed
	Anderson R, Wilkinson J. Letter: Isolated ventricular inversion with situs solitus. Br Heart J. 1975;37:1202 pubmed

product information

Internal ID :

3327

Name :

Depositor Name :

Lytle, C. / Forbush III, B.

Depositor Institution :

Yale University

Date Deposited :

2/2/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Na-K-Cl cotransporter

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Avian,Elasmobranch,Mammal,Sheep,Xenopus

Species Tested (legacy) :

mammalian, avian, elasmobranch (mAb T4)

Initial Publication Pubmed ID :

8572179

Depositor Notes (Special Instructions) :

Antigen retrieval (1% SDS for 5 min) required for immunostaining frozen sections. T4 recognizes NKCC1 and NKCC2 on western blots and by IF, but is weak for NKCC2 by IF (PMID: 8572179)

Collections :

Cell signaling,Human,Neurodevelopment

Search Keywords :

Lytle/Forbush III, Na-K-Cl cotransporters, NKCC1, SLC12A1, SLC12A2, monoclonal, human, AB_528406, IgG1, Avian,Elasmobranch,Mammal,Xenopus, FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Antigen Molecular Weight :

Predicted: 131.4kDa Apparent: 145-205kDa

Gene :

SLC12A1, SLC12A2

Alternate Gene Name(s) :

NKCC1 and NKCC2

Uniprot ID :

P55011 Q13621

Antibody Registry ID :

AB_528406

Immunogen :

Recombinant 38kDa peptide fragment of carboxy terminus encoded by cDNA from T84 (human) epithelial Na-K-Cl cotransport protein

Immunogen Sequence :

human NKCC1 C-terminus (Met902-Ser1212)

Clonality :

Monoclonal

Myeloma Strain :

P3x63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

a.a. 902-1212

Recommended Applications :

FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

16959864 18455940 31415088

Immunofluorescence Pubmed IDs :

8572179 8910547 8764311 1191438 11948203 15767308 15914646 16215213 17337714 18455940 19864322 11104718 24990918

Western Blot Pubmed IDs :

8572179 11910357 24990918 12115907 11948203 15562450 17337714 11104718

Immunoprecipitation Pubmed IDs :

8572179 11104718

FFPE Pubmed IDs :

18800228

Depositor Growth Medium :

RPMI 1640 medium plus 15% FCS plus pen-strep

Additional Information :

Paraffin embedment of ethanol fixed tissue.

DSHB Growth Medium :

RPMI

References (legacy) :

Am. J. Physiol. 269, C1496-C1505.; Gastroenterology 122(4), 1058-1069.; J. Exp. Zool. 293, 106-118.; J. Exp. Biol. 205, 745-758.; J. Exp. Biol. 205, 1265-1273.; J. Exp. Zool. 301A, 979-991.; J. Exp. Biol. 208, 1063-1077.; J. Exp. Biol. 208, 2023-2036.; J. Exp. Biol. 208, 3851-3858.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 292, 535-543.; Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 148, 29-43.; Comp. Biochem. Physiol. Part D Genomics Proteomics 2, 257-286.; J. Exp. Biol. 210, 1015-1024.; J. Exp. Biol. 211, 2584-2599.; Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 150, 288-300.; Cell Tissue Res. 334, 265-281.; Am. J. Physiol. Cell Physiol. 298, C85-C97.

more info or order :

Developmental Studies Hybridoma Bank product webpage