product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Smoothened (Smo)
catalog :
Smoothened (20C6)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Smoothened (20C6)
reactivity :
fruit fly
application :
immunocytochemistry, immunoprecipitation
more info or order :
citations: 12
Reference |
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Claret S, Sanial M, Plessis A. Evidence for a novel feedback loop in the Hedgehog pathway involving Smoothened and Fused. Curr Biol. 2007;17:1326-33 pubmed
|
Malpel S, Claret S, Sanial M, Brigui A, Piolot T, Daviet L, et al. The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway. Dev Biol. 2007;303:121-33 pubmed
|
Vrailas A, Moses K. Smoothened, thickveins and the genetic control of cell cycle and cell fate in the developing Drosophila eye. Mech Dev. 2006;123:151-65 pubmed
|
Zhang C, Williams E, Guo Y, Lum L, Beachy P. Extensive phosphorylation of Smoothened in Hedgehog pathway activation. Proc Natl Acad Sci U S A. 2004;101:17900-7 pubmed
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Lum L, Zhang C, Oh S, Mann R, von Kessler D, Taipale J, et al. Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2. Mol Cell. 2003;12:1261-74 pubmed
|
Blahovec J. Stress relaxation in cherry fruit. Biorheology. 1996;33:451-62 pubmed
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product information
Internal ID :
743
Name :
Smoothened (20C6)
Depositor Name :
Beachy, P.A.
Depositor Institution :
Stanford University
Date Deposited :
10/21/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Smoothened (Smo)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
14636583
Depositor Notes (Special Instructions) :
Doesn't appear to work for Immunoblotting.
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Beachy, P.A., Smoothened (Smo), Drosophila, MIgG1, Drosophila, smo, CG11561, AB_528472, monoclonal, Cell signaling/Drosophila antigens, Immunofluorescence/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 116 kDa
Gene :
smo
Alternate Gene Name(s) :
CG11561
Uniprot ID :
P91682
Antibody Registry ID :
AB_528472
Immunogen :
Recombinant N-terminal first extracellular domain (aa 1-257) fused in-frame with the Fc region of human IgG
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
N-terminal first extracellular domain (aa 1-257)
Epitope Map PubMed ID :
14636583
Recommended Applications :
Immunofluorescence,Immunoprecipitation
Immunoblotting (legacy) :
No
Immunofluorescence Pubmed IDs :
14636583 22537496 16412615 21730325 17658259 17182028 25289679 23385085 22306085 23453925 9093440 31010679
Immunoprecipitation Pubmed IDs :
15598741 25289679 14636583
DSHB Growth Medium :
Iscove's
References (legacy) :
Mol. Cell. 12(5), 1261-1274.; Dev. Biol. 366, 172-184.
more info or order :
company information
Developmental Studies Hybridoma Bank
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