product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Smoothened (Smo)
catalog :
Smoothened (20C6)
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Smoothened (20C6)
reactivity :
fruit fly
application :
immunocytochemistry, immunoprecipitation
citations: 12
Reference
Ranieri N, Therond P, Ruel L. Switch of PKA substrates from Cubitus interruptus to Smoothened in the Hedgehog signalosome complex. Nat Commun. 2014;5:5034 pubmed publisher
Gao L, Wu L, Hou X, Zhang Q, Zhang F, Ye X, et al. Drosophila miR-932 modulates hedgehog signaling by targeting its co-receptor Brother of ihog. Dev Biol. 2013;377:166-76 pubmed publisher
Gao L, Hou X, Wu L, Zhang F, Zhang Q, Ye X, et al. Drosophila miR-960 negatively regulates Hedgehog signaling by suppressing Smoothened, Costal-2 and Fused. Cell Signal. 2013;25:1301-9 pubmed publisher
Fan J, Liu Y, Jia J. Hh-induced Smoothened conformational switch is mediated by differential phosphorylation at its C-terminal tail in a dose- and position-dependent manner. Dev Biol. 2012;366:172-84 pubmed publisher
Ranieri N, Ruel L, Gallet A, Raisin S, Therond P. Distinct phosphorylations on kinesin costal-2 mediate differential hedgehog signaling strength. Dev Cell. 2012;22:279-94 pubmed publisher
Su Y, Ospina J, Zhang J, Michelson A, Schoen A, Zhu A. Sequential phosphorylation of smoothened transduces graded hedgehog signaling. Sci Signal. 2011;4:ra43 pubmed publisher
Claret S, Sanial M, Plessis A. Evidence for a novel feedback loop in the Hedgehog pathway involving Smoothened and Fused. Curr Biol. 2007;17:1326-33 pubmed
Malpel S, Claret S, Sanial M, Brigui A, Piolot T, Daviet L, et al. The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway. Dev Biol. 2007;303:121-33 pubmed
Vrailas A, Moses K. Smoothened, thickveins and the genetic control of cell cycle and cell fate in the developing Drosophila eye. Mech Dev. 2006;123:151-65 pubmed
Zhang C, Williams E, Guo Y, Lum L, Beachy P. Extensive phosphorylation of Smoothened in Hedgehog pathway activation. Proc Natl Acad Sci U S A. 2004;101:17900-7 pubmed
Lum L, Zhang C, Oh S, Mann R, von Kessler D, Taipale J, et al. Hedgehog signal transduction via Smoothened association with a cytoplasmic complex scaffolded by the atypical kinesin, Costal-2. Mol Cell. 2003;12:1261-74 pubmed
Blahovec J. Stress relaxation in cherry fruit. Biorheology. 1996;33:451-62 pubmed
product information
Internal ID :
743
Name :
Smoothened (20C6)
Depositor Name :
Beachy, P.A.
Depositor Institution :
Stanford University
Date Deposited :
10/21/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Smoothened (Smo)
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
14636583
Depositor Notes (Special Instructions) :
Doesn't appear to work for Immunoblotting.
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Beachy, P.A., Smoothened (Smo), Drosophila, MIgG1, Drosophila, smo, CG11561, AB_528472, monoclonal, Cell signaling/Drosophila antigens, Immunofluorescence/Immunoprecipitation
Antigen Molecular Weight :
Predicted: 116 kDa
Gene :
smo
Alternate Gene Name(s) :
CG11561
Uniprot ID :
P91682
Antibody Registry ID :
AB_528472
Immunogen :
Recombinant N-terminal first extracellular domain (aa 1-257) fused in-frame with the Fc region of human IgG
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
N-terminal first extracellular domain (aa 1-257)
Epitope Map PubMed ID :
14636583
Recommended Applications :
Immunofluorescence,Immunoprecipitation
Immunoblotting (legacy) :
No
Immunofluorescence Pubmed IDs :
14636583 22537496 16412615 21730325 17658259 17182028 25289679 23385085 22306085 23453925 9093440 31010679
Immunoprecipitation Pubmed IDs :
15598741 25289679 14636583
DSHB Growth Medium :
Iscove's
References (legacy) :
Mol. Cell. 12(5), 1261-1274.; Dev. Biol. 366, 172-184.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US