SUMO2 (SUMO-2/3) | Developmental Studies Hybridoma Bank SUMO-2 8A2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SUMO2 (SUMO-2/3)

catalog :

SUMO-2 8A2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SUMO-2 8A2

reactivity :

human, mouse, chicken, zebrafish

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Rosen J, Azevedo M, Soffar D, Boyko V, Brendel M, Schulman V, et al. The Drosophila Ninein homologue Bsg25D cooperates with Ensconsin in myonuclear positioning. J Cell Biol. 2019;218:524-540 pubmed publisher
Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, et al. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission. Nat Commun. 2016;7:13365 pubmed publisher
Fujiwara K, Hasegawa K, Oka M, Yoneda Y, Yoshikawa K. Terminal differentiation of cortical neurons rapidly remodels RanGAP-mediated nuclear transport system. Genes Cells. 2016;21:1176-1194 pubmed publisher
Ristic M, Brockly F, Piechaczyk M, Bossis G. Detection of Protein-Protein Interactions and Posttranslational Modifications Using the Proximity Ligation Assay: Application to the Study of the SUMO Pathway. Methods Mol Biol. 2016;1449:279-90 pubmed publisher
Antunica Noguerol M, BudziÃ±ski M, Druker J, Gassen N, Sokn M, Senin S, et al. The activity of the glucocorticoid receptor is regulated by SUMO conjugation to FKBP51. Cell Death Differ. 2016;23:1579-91 pubmed publisher
Juárez Vicente F, Luna Pelaez N, Garcia Dominguez M. The Sumo protease Senp7 is required for proper neuronal differentiation. Biochim Biophys Acta. 2016;1863:1490-8 pubmed publisher
Eisenhardt N, Chaugule V, Koidl S, Droescher M, Dogan E, Rettich J, et al. A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly. Nat Struct Mol Biol. 2015;22:959-67 pubmed publisher
Bossis G, Sarry J, Kifagi C, Ristic M, Saland E, Vergez F, et al. The ROS/SUMO axis contributes to the response of acute myeloid leukemia cells to chemotherapeutic drugs. Cell Rep. 2014;7:1815-23 pubmed publisher
Barysch S, Dittner C, Flotho A, Becker J, Melchior F. Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies. Nat Protoc. 2014;9:896-909 pubmed publisher
Becker J, Barysch S, Karaca S, Dittner C, Hsiao H, Berriel Diaz M, et al. Detecting endogenous SUMO targets in mammalian cells and tissues. Nat Struct Mol Biol. 2013;20:525-31 pubmed publisher
Zhang X, Goeres J, Zhang H, Yen T, Porter A, Matunis M. SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Mol Cell. 2008;29:729-41 pubmed publisher

product information

Internal ID :

5950

Name :

SUMO-2 8A2

Depositor Name :

Matunis, M.

Depositor Institution :

Johns Hopkins University School of Medicine

Date Deposited :

4/27/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SUMO2 (SUMO-2/3)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Drosophila melanogaster,Human,Mouse,Xenopus,Zebrafish

Species Tested (legacy) :

all vertebrates tested

Initial Publication Pubmed ID :

18374647

Depositor Notes (Special Instructions) :

This antibody recognizes SUMO2 and SUMO3 and their conjugates in all vertebrate cells tested; does not cross-react with SUMO1 .

Collections :

Cell signaling,Drosophila antigens,Human,Xenopus

Search Keywords :

Matunis, M., SUMO2 (SUMO-2/3), Human, MIgG1, Human/Mouse/Chicken/Xenopus/Zebrafish/Drosophila melanogaster, SUMO2, SMT3B, SMT3H2, AB_2198421, monoclonal, Cell signaling/Human/Xenopus/Drosophila antigens, Immunoprecipitation/Immunofluorescence/Western Blot

Antigen Molecular Weight :

11 kDa

Gene :

SUMO2

Alternate Gene Name(s) :

SMT3B, SMT3H2

Uniprot ID :

P61956

Antibody Registry ID :

AB_2198421

Synonyms (Alternate Clone Names) :

8A2

Immunogen :

Recombinant SUMO-2 (GenBank accession number P61956) protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 57-66, IRFRFDGQPI

Epitope Map PubMed ID :

23503365

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

18374647 27177020

Western Blot Pubmed IDs :

18374647 23503365 26524493 27717094 27039038 24651501 24910433

Immunoprecipitation Pubmed IDs :

23503365 27177020 26524493 24651501

Pubmed IDs :

27613043 27819299

Additional Information :

This antibody will not recognize isoform 2 of SUMO-2 missing aa 51-74, but will recognize both SUMO-3 isoforms.

DSHB Growth Medium :

Iscove's

References (legacy) :

Mol. Cell 29, 729-741.; Nat. Struct. Mol. Biol. 20, 525-531.

more info or order :

Developmental Studies Hybridoma Bank product webpage