SUMO1, aa 57-67 | Developmental Studies Hybridoma Bank SUMO-1 21C7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SUMO1, aa 57-67

catalog :

SUMO-1 21C7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SUMO-1 21C7

reactivity :

human, mouse, rat, chicken, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 19

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; fig 1 western blot; mouse; fig 1	Zhang L, Liu X, Sheng H, Liu S, Li Y, Zhao J, et al. Neuron-specific SUMO knockdown suppresses global gene expression response and worsens functional outcome after transient forebrain ischemia in mice. Neuroscience. 2017;343:190-212 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 1b	Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, et al. Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission. Nat Commun. 2016;7:13365 pubmed publisher
western blot; mouse; 1:1000; fig 1	Juárez Vicente F, Luna Pelaez N, Garcia Dominguez M. The Sumo protease Senp7 is required for proper neuronal differentiation. Biochim Biophys Acta. 2016;1863:1490-8 pubmed publisher
immunocytochemistry; rat; 1:250; fig 1	Craig T, Anderson D, Evans A, Girach F, Henley J. SUMOylation of Syntaxin1A regulates presynaptic endocytosis. Sci Rep. 2015;5:17669 pubmed publisher
western blot; human; fig 2	Mendes A, Grou C, Azevedo J, Pinto M. Evaluation of the activity and substrate specificity of the human SENP family of SUMO proteases. Biochim Biophys Acta. 2016;1863:139-47 pubmed publisher
western blot; rat	Blakeslee W, Wysoczynski C, Fritz K, Nyborg J, Churchill M, McKinsey T. Class I HDAC inhibition stimulates cardiac protein SUMOylation through a post-translational mechanism. Cell Signal. 2014;26:2912-20 pubmed publisher
western blot; human	Berndt A, Wilkinson K, Heimann M, Bishop P, Henley J. In vivo characterization of the properties of SUMO1-specific monobodies. Biochem J. 2013;456:385-95 pubmed publisher
	Wan J, Block S, Scribano C, Thiry R, Esbona K, Audhya A, et al. Mad1 destabilizes p53 by preventing PML from sequestering MDM2. Nat Commun. 2019;10:1540 pubmed publisher
	Wen D, Wu J, Wang L, Fu Z. SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function. Cell Rep. 2017;21:2147-2159 pubmed publisher
	Chanda A, Chan A, Deng L, Kornaga E, Enwere E, Morris D, et al. Identification of the SUMO E3 ligase PIAS1 as a potential survival biomarker in breast cancer. PLoS ONE. 2017;12:e0177639 pubmed publisher
	Fujiwara K, Hasegawa K, Oka M, Yoneda Y, Yoshikawa K. Terminal differentiation of cortical neurons rapidly remodels RanGAP-mediated nuclear transport system. Genes Cells. 2016;21:1176-1194 pubmed publisher
	Ristic M, Brockly F, Piechaczyk M, Bossis G. Detection of Protein-Protein Interactions and Posttranslational Modifications Using the Proximity Ligation Assay: Application to the Study of the SUMO Pathway. Methods Mol Biol. 2016;1449:279-90 pubmed publisher
	Eisenhardt N, Chaugule V, Koidl S, Droescher M, Dogan E, Rettich J, et al. A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly. Nat Struct Mol Biol. 2015;22:959-67 pubmed publisher
	Bossis G, Sarry J, Kifagi C, Ristic M, Saland E, Vergez F, et al. The ROS/SUMO axis contributes to the response of acute myeloid leukemia cells to chemotherapeutic drugs. Cell Rep. 2014;7:1815-23 pubmed publisher
	Barysch S, Dittner C, Flotho A, Becker J, Melchior F. Identification and analysis of endogenous SUMO1 and SUMO2/3 targets in mammalian cells and tissues using monoclonal antibodies. Nat Protoc. 2014;9:896-909 pubmed publisher
	Becker J, Barysch S, Karaca S, Dittner C, Hsiao H, Berriel Diaz M, et al. Detecting endogenous SUMO targets in mammalian cells and tissues. Nat Struct Mol Biol. 2013;20:525-31 pubmed publisher
	Zhang X, Goeres J, Zhang H, Yen T, Porter A, Matunis M. SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis. Mol Cell. 2008;29:729-41 pubmed publisher
	Rogers R, Inselman A, Handel M, Matunis M. SUMO modified proteins localize to the XY body of pachytene spermatocytes. Chromosoma. 2004;113:233-43 pubmed
	Matunis M, Coutavas E, Blobel G. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J Cell Biol. 1996;135:1457-70 pubmed

product information

Internal ID :

5944

Name :

SUMO-1 21C7

Depositor Name :

Matunis, M.

Depositor Institution :

Johns Hopkins University School of Medicine

Date Deposited :

4/27/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SUMO1, aa 57-67

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Human,Mouse,Rat,Xenopus,Zebrafish

Species Tested (legacy) :

all vertebrates tested

Initial Publication Pubmed ID :

8978815

Depositor Notes (Special Instructions) :

This antibody recognizes SUMO1 and SUMO1 conjugates in all vertebrate cells tested; does not cross-react with SUMO2 or SUMO3. 21C7 doesn't recognize C. elegans SUMO1.

Collections :

Cell signaling,Human,Xenopus

Search Keywords :

Matunis, M., SUMO1, aa 57-67, Human, MIgG1, Human/Rat/Mouse/Zebrafish/Xenopus/Chicken, SUMO1, SMT3C, SMT3H3, UBL1, PIC1, DAP1, GMP1, OFC10, SENP2, AB_2198257, monoclonal, Cell signaling/Human/Xenopus, Immunoprecipitation/Immunofluorescence/Western Blot

Antigen Molecular Weight :

11 kDa

Gene :

SUMO1

Alternate Gene Name(s) :

SMT3C, SMT3H3, UBL1, PIC1, DAP1, GMP1, OFC10, SENP2

Uniprot ID :

P63165

Antibody Registry ID :

AB_2198257

Synonyms (Alternate Clone Names) :

21C7

Immunogen :

Recombinant SUMO-1 (GenBank accession number AAB40390) protein with N-terminal 6xHis tag

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/0-Ag14

Epitope Mapped :

Yes

Epitope Location or Sequence :

aa 57-67 VPMNSLRFLFE

Epitope Map PubMed ID :

23503365

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

30948704

Immunofluorescence Pubmed IDs :

8978815 18374647 15349788 30948704

Western Blot Pubmed IDs :

8978815 28493978 18374647 15349788 23503365 26524493 27717094 27039038 24651501 24910433 30948704

Immunoprecipitation Pubmed IDs :

23503365 24651501

FFPE Pubmed IDs :

30948704

Pubmed IDs :

27613043 27819299

Additional Information :

This antibody will recognize both SUMO-1 isoforms.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell. Biol. 135, 1457-1470.; Chromosoma 113, 233-243.; Mol. Cell 29, 729-741.; Nat. Struct. Mol. Biol. 20, 525-531.

more info or order :

Developmental Studies Hybridoma Bank product webpage