product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
kinesin heavy chain, tail region
catalog :
SUK 5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
SUK 5
reactivity :
purple sea urchin
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 6
Reference
De Vos K, Goossens V, Boone E, Vercammen D, Vancompernolle K, Vandenabeele P, et al. The 55-kDa tumor necrosis factor receptor induces clustering of mitochondria through its membrane-proximal region. J Biol Chem. 1998;273:9673-80 pubmed
Ingold A, Cohn S, Scholey J. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J Cell Biol. 1988;107:2657-67 pubmed
Wright B, Henson J, Wedaman K, Willy P, Morand J, Scholey J. Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm. J Cell Biol. 1991;113:817-33 pubmed
Houliston E, Elinson R. Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs. J Cell Biol. 1991;114:1017-28 pubmed
Cole D, Cande W, Baskin R, Skoufias D, Hogan C, Scholey J. Isolation of a sea urchin egg kinesin-related protein using peptide antibodies. J Cell Sci. 1992;101 ( Pt 2):291-301 pubmed
Henson J, Nesbitt D, Wright B, Scholey J. Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles. J Cell Sci. 1992;103 ( Pt 2):309-20 pubmed
product information
Internal ID :
2084
Name :
SUK 5
Depositor Name :
Scholey, J.M.
Depositor Institution :
University of California, Davis
Date Deposited :
9/12/91
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
kinesin heavy chain, tail region
Antigen Species :
Sea Urchin
Host Species :
mouse
Isotype :
MIgG2b
Isotype for catalog (legacy) :
IgG2b
Positive Tested Species Reactivity :
Bovine,Mouse,Sea Urchin,Xenopus
Species Tested (legacy) :
urchin, bovine
Initial Publication Pubmed ID :
2974459
Depositor Notes (Special Instructions) :
SUK 5 doesn't cross react with squid or Drosophila kinesins.
Collections :
Cytoskeleton,Enzymes,Xenopus
Search Keywords :
Scholey, J.M., kinesin heavy chain, tail region, See Urchin, MIgG2b, sea urchin/bovine/Xenopus/Mouse, , , AB_528327, monoclonal, Cytoskeletal elements/Enzymes/Xenopus, Immunohistochemistry/Immunoprecipitation/Immunofluorescence/Western Blot
Antigen Molecular Weight :
Predicted: 118 kDa; Apparent: 130 kDa
Uniprot ID :
P35978
Antibody Registry ID :
AB_528327
Immunogen :
Partially purified native kinesin from see urchin egg cytosol
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
P3x635-68.653
Epitope Mapped :
Yes
Epitope Location or Sequence :
Tail region of kinesin heavy chain (aa 859-1031)
Epitope Map PubMed ID :
1827446
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunohistochemistry Pubmed IDs :
1827446
Immunofluorescence Pubmed IDs :
1714912 1478935
Western Blot Pubmed IDs :
2974459 1827446 1629246 1714912 1478935 9545301
Immunoprecipitation Pubmed IDs :
2974459
Additional Information :
In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Bio. 107, 2657-2667.; Nature 338, 355-357.; J. Cell Science 14s, 109-115.; J. Cell Biol. 113, 817-833.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US