kinesin heavy chain, head region | Developmental Studies Hybridoma Bank SUK 4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

kinesin heavy chain, head region

catalog :

SUK 4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SUK 4

reactivity :

human, fruit fly, purple sea urchin

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; loading ...; fig 3c immunocytochemistry; human; loading ...; fig 3a	Randall T, Yip Y, Wallock Richards D, Pfisterer K, Sanger A, Ficek W, et al. A small-molecule activator of kinesin-1 drives remodeling of the microtubule network. Proc Natl Acad Sci U S A. 2017;114:13738-13743 pubmed publisher
immunocytochemistry; fruit fly; 1:100	Li L, Tian X, Zhu M, Bulgari D, Böhme M, Goettfert F, et al. Drosophila Syd-1, liprin-α, and protein phosphatase 2A B' subunit Wrd function in a linear pathway to prevent ectopic accumulation of synaptic materials in distal axons. J Neurosci. 2014;34:8474-87 pubmed publisher
	Weiss K, Littleton J. Characterization of axonal transport defects in Drosophila Huntingtin mutants. J Neurogenet. 2016;30:212-221 pubmed
	CYRUS B, Muller W. A Unique Role for Endothelial Cell Kinesin Light Chain 1, Variant 1 in Leukocyte Transendothelial Migration. Am J Pathol. 2016;186:1375-86 pubmed publisher
	Iacobucci G, Rahman N, Valtueña A, Nayak T, Gunawardena S. Spatial and temporal characteristics of normal and perturbed vesicle transport. PLoS ONE. 2014;9:e97237 pubmed publisher
	Xu J, Reddy B, Anand P, Shu Z, Cermelli S, Mattson M, et al. Casein kinase 2 reverses tail-independent inactivation of kinesin-1. Nat Commun. 2012;3:754 pubmed publisher
	Silver K, Harrison R. Kinesin 5B is necessary for delivery of membrane and receptors during Fc?R-mediated phagocytosis. J Immunol. 2011;186:816-25 pubmed publisher
	Jolly A, Kim H, Srinivasan D, Lakonishok M, Larson A, Gelfand V. Kinesin-1 heavy chain mediates microtubule sliding to drive changes in cell shape. Proc Natl Acad Sci U S A. 2010;107:12151-6 pubmed publisher
	Hamanaka Y, Meinertzhagen I. Immunocytochemical localization of synaptic proteins to photoreceptor synapses of Drosophila melanogaster. J Comp Neurol. 2010;518:1133-55 pubmed publisher
	Ally S, Larson A, Barlan K, Rice S, Gelfand V. Opposite-polarity motors activate one another to trigger cargo transport in live cells. J Cell Biol. 2009;187:1071-82 pubmed publisher
	Cardoso C, Groth Pedersen L, Høyer Hansen M, Kirkegaard T, Corcelle E, Andersen J, et al. Depletion of kinesin 5B affects lysosomal distribution and stability and induces peri-nuclear accumulation of autophagosomes in cancer cells. PLoS ONE. 2009;4:e4424 pubmed publisher
	Messitt T, Gagnon J, Kreiling J, Pratt C, Yoon Y, Mowry K. Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes. Dev Cell. 2008;15:426-36 pubmed publisher
	Robb S, SANCHEZ ALVARADO A. Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy. Genesis. 2002;32:293-8 pubmed
	Kanai Y, Okada Y, Tanaka Y, Harada A, Terada S, Hirokawa N. KIF5C, a novel neuronal kinesin enriched in motor neurons. J Neurosci. 2000;20:6374-84 pubmed
	De Vos K, Goossens V, Boone E, Vercammen D, Vancompernolle K, Vandenabeele P, et al. The 55-kDa tumor necrosis factor receptor induces clustering of mitochondria through its membrane-proximal region. J Biol Chem. 1998;273:9673-80 pubmed
	Bi G, Morris R, Liao G, Alderton J, Scholey J, Steinhardt R. Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis. J Cell Biol. 1997;138:999-1008 pubmed
	Skoufias D, Cole D, Wedaman K, Scholey J. The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding. J Biol Chem. 1994;269:1477-85 pubmed
	Steinhardt R, Bi G, Alderton J. Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release. Science. 1994;263:390-3 pubmed
	Ingold A, Cohn S, Scholey J. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J Cell Biol. 1988;107:2657-67 pubmed
	Scholey J, Heuser J, Yang J, Goldstein L. Identification of globular mechanochemical heads of kinesin. Nature. 1989;338:355-7 pubmed
	Johnson C, Buster D, Scholey J. Light chains of sea urchin kinesin identified by immunoadsorption. Cell Motil Cytoskeleton. 1990;16:204-13 pubmed
	Buster D, Scholey J. Purification and assay of kinesin from sea urchin eggs and early embryos. J Cell Sci Suppl. 1991;14:109-15 pubmed
	Wright B, Henson J, Wedaman K, Willy P, Morand J, Scholey J. Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm. J Cell Biol. 1991;113:817-33 pubmed
	Houliston E, Elinson R. Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs. J Cell Biol. 1991;114:1017-28 pubmed
	Sawin K, Mitchison T, Wordeman L. Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies. J Cell Sci. 1992;101 ( Pt 2):303-13 pubmed
	Cole D, Cande W, Baskin R, Skoufias D, Hogan C, Scholey J. Isolation of a sea urchin egg kinesin-related protein using peptide antibodies. J Cell Sci. 1992;101 ( Pt 2):291-301 pubmed
	Henson J, Nesbitt D, Wright B, Scholey J. Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles. J Cell Sci. 1992;103 ( Pt 2):309-20 pubmed

product information

Internal ID :

2078

Name :

SUK 4

Depositor Name :

Scholey, J.M.

Depositor Institution :

University of California, Davis

Date Deposited :

9/12/91

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

kinesin heavy chain, head region

Antigen Species :

Sea Urchin

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Canine,Drosophila,Human,Mouse,Planaria,Sea Urchin,Xenopus

Species Tested (legacy) :

urchin, bovine, Drosophila, planaria, canine, human

Initial Publication Pubmed ID :

2974459

Depositor Notes (Special Instructions) :

Inhibits sea urchin egg kinesin-induced microtubule translocation. SUK 4 doesn't cross react with squid kinesin.

Collections :

Cytoskeleton,Drosophila antigens,Enzymes,Human,Xenopus

Search Keywords :

Scholey, J.M., kinesin heavy chain, head region, See Urchin, MIgG1, sea urchin/bovine/Drosophila/planaria/canine/human/Xenopus/Mouse, , , AB_528326, monoclonal, Cytoskeletal elements/Drosophila antigens/Enzymes/Human/Xenopus, Immunohistochemistry/Immunoprecipitation/Function Blocking/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 118 kDa; Apparent: 130 kDa

Uniprot ID :

P35978

Antibody Registry ID :

AB_528326

Immunogen :

Partially purified native kinesin from see urchin egg cytosol

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3x635-68.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

Head region of kinesin heavy chain containing the microtubule-binding region. The epitope is located on aa 336-390 of the Drosophila kinesin heavy chain which corresponds to aa 328-382 of the urchin protein.

Epitope Map PubMed ID :

2493586

Recommended Applications :

Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunohistochemistry Pubmed IDs :

1827446

Immunofluorescence Pubmed IDs :

11948917 22453827 24878565 24948803 21149599 20127822 1714912 1478935 18771961 9545301

Western Blot Pubmed IDs :

2974459 2493586 1827446 10964943 27309588 1629246 2142020 1629247 1714912 1478935 8288613 9545301

Immunoprecipitation Pubmed IDs :

2974459 2493586 1832162 22453827 1629246 2142020 18771961

Function Blocking Pubmed IDs :

2974459 1832162 26994343 20566873 7904084 9281579 18771961

Additional Information :

In mice, SUK 4 recognizes the three kinesin-1 KIF5A, KIF5B and KIF5C, with a more intense stainning for KIF5A [PMID: 10964943]. In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol 107, 2657-2667.; Nature 338, 355-357.; J. Cell Science 14s, 109-115.; J. Cell Biol. 113, 817-833.; Genesis 32, 293-298.

more info or order :

Developmental Studies Hybridoma Bank product webpage