kinesin heavy chain, stalk region | Developmental Studies Hybridoma Bank SUK 2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

kinesin heavy chain, stalk region

catalog :

SUK 2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SUK 2

reactivity :

purple sea urchin

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 9

Reference
Skoufias D, Cole D, Wedaman K, Scholey J. The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding. J Biol Chem. 1994;269:1477-85 pubmed
Ingold A, Cohn S, Scholey J. Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J Cell Biol. 1988;107:2657-67 pubmed
Johnson C, Buster D, Scholey J. Light chains of sea urchin kinesin identified by immunoadsorption. Cell Motil Cytoskeleton. 1990;16:204-13 pubmed
Buster D, Scholey J. Purification and assay of kinesin from sea urchin eggs and early embryos. J Cell Sci Suppl. 1991;14:109-15 pubmed
Wright B, Henson J, Wedaman K, Willy P, Morand J, Scholey J. Subcellular localization and sequence of sea urchin kinesin heavy chain: evidence for its association with membranes in the mitotic apparatus and interphase cytoplasm. J Cell Biol. 1991;113:817-33 pubmed
Houliston E, Elinson R. Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs. J Cell Biol. 1991;114:1017-28 pubmed
Sawin K, Mitchison T, Wordeman L. Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies. J Cell Sci. 1992;101 ( Pt 2):303-13 pubmed
Cole D, Cande W, Baskin R, Skoufias D, Hogan C, Scholey J. Isolation of a sea urchin egg kinesin-related protein using peptide antibodies. J Cell Sci. 1992;101 ( Pt 2):291-301 pubmed
Henson J, Nesbitt D, Wright B, Scholey J. Immunolocalization of kinesin in sea urchin coelomocytes. Association of kinesin with intracellular organelles. J Cell Sci. 1992;103 ( Pt 2):309-20 pubmed

product information

Internal ID :

2072

Name :

SUK 2

Depositor Name :

Scholey, J.M.

Depositor Institution :

University of California, Davis

Date Deposited :

9/12/91

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

kinesin heavy chain, stalk region

Antigen Species :

Sea Urchin

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Sea Urchin,Xenopus

Species Tested (legacy) :

urchin

Initial Publication Pubmed ID :

2974459

Depositor Notes (Special Instructions) :

SUK 2 doesn't cross react with squid, bovine or Drosophila kinesins.

Collections :

Cytoskeleton,Enzymes,Xenopus

Search Keywords :

Scholey, J.M., kinesin heavy chain, stalk region, See Urchin, MIgG1, sea urchin/Xenopus, , , AB_528325, monoclonal, Cytoskeletal elements/Enzymes/Xenopus, Immunohistochemistry/Immunoprecipitation/Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: 118 kDa; Apparent: 130 kDa

Uniprot ID :

P35978

Antibody Registry ID :

AB_528325

Immunogen :

Partially purified native kinesin from see urchin egg cytosol

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3x635-68.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

Stalk region of kinesin heavy chain (aa 578-858)

Epitope Map PubMed ID :

1827446

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunohistochemistry Pubmed IDs :

1827446

Immunofluorescence Pubmed IDs :

1629247 1714912 1478935

Western Blot Pubmed IDs :

2974459 1827446 1629246 2142020 1629247 1714912 1478935 8288613

Immunoprecipitation Pubmed IDs :

2974459 1832162 2142020

Additional Information :

In any publications that result from the use of SUK 2, 4, or 5 antibody, Dr. Jonathan Scholey requests that the following be cited: Ingold, A.L., Cohn, S.A., and Scholey, J.M. (1988). Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains. J. Cell Biol. 107, 2657-3667.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 107, 2657-2667.; Nature 338, 355-357.; J. Cell Science 14s, 109-115.; J. Cell Biol. 113, 817-833.

more info or order :

Developmental Studies Hybridoma Bank product webpage