SPARC (secreted protein acidic cysteine-rich) /osteonectin/BM-40 | Developmental Studies Hybridoma Bank SPARC mAb 236 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

SPARC (secreted protein acidic cysteine-rich) /osteonectin/BM-40

catalog :

SPARC mAb 236

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SPARC mAb 236

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

product information

Internal ID :

8917

Name :

SPARC mAb 236

Depositor Name :

Brekken, R.A.

Depositor Institution :

UT Southwestern Medical Center,

Depositor Website :

http://www.utsouthwestern.edu/labs/brekken/

Date Deposited :

1/13/13

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

SPARC (secreted protein acidic cysteine-rich) /osteonectin/BM-40

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

human, weak in mouse

Initial Publication Pubmed ID :

15150281

Depositor Notes (Special Instructions) :

This antibody blocks the formation of the SPARC-beta 1 integrin complex. Fixation: frozen, formalin and methy Carnoy's

Collections :

Extracellular matrix proteins,Human

Search Keywords :

Brekken, R.A., SPARC (secreted protein acidic cysteine-rich) /osteonectin/BM-40, Human, MIgG1, kappa light chain, Human/Mouse, SPARC, ON, AB_2617208, Monoclonal , Extracellular matrix proteins/Human, Western blot/Immunohistochemistry/ELISA/FFPE/Function Blocking

Antigen Molecular Weight :

43 kDa

Gene :

SPARC

Alternate Gene Name(s) :

Uniprot ID :

P09486

Antibody Registry ID :

AB_2617208

Immunogen :

recombinant SPARC injected into SPARC-null mice

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3-X63-Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

Follistatin domain including SPARC copper binding sequence (KKGHK)

Epitope Map PubMed ID :

18503049

Recommended Applications :

ELISA,FFPE,Function Blocking,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

15150281

Western Blot Pubmed IDs :

15150281

ELISA Pubmed IDs :

15150281 18503049

FFPE Pubmed IDs :

15150281

Function Blocking Pubmed IDs :

15150281

Depositor Growth Medium :

Iscove’s modified Dulbecco’s medium with 10% Fetal Calf serum

Additional Information :

RRID: AB_2617208

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Histochem. Cytochem. 52, 723-733.; J. Biol. Chem. 283(33), 22826-22837.

more info or order :

Developmental Studies Hybridoma Bank product webpage