Collagen, Type I pro-peptide | Developmental Studies Hybridoma Bank SP1.D8 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Collagen, Type I pro-peptide

catalog :

SP1.D8

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SP1.D8

reactivity :

human, mouse, brown rat, dogs, bantam, bovine, leopard danio

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 39

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:20; loading ...; fig 1a	Tran M, Tsutsumi R, Erberich J, Chen K, Flores M, Cooper K. Evolutionary loss of foot muscle during development with characteristics of atrophy and no evidence of cell death. elife. 2019;8: pubmed publisher
immunocytochemistry; human; loading ...; fig 1b western blot; human; loading ...; fig s1j immunocytochemistry; mouse; loading ...; fig 6h western blot; mouse; loading ...; fig 6i	Subramanian A, Capalbo A, Iyengar N, Rizzo R, Di Campli A, Di Martino R, et al. Auto-regulation of Secretory Flux by Sensing and Responding to the Folded Cargo Protein Load in the Endoplasmic Reticulum. Cell. 2019;176:1461-1476.e23 pubmed publisher
immunocytochemistry; bantam; fig 1	Boswell B, Korol A, West Mays J, Musil L. Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract. Mol Biol Cell. 2017;28:907-921 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig s2	Nazari B, Rice L, Stifano G, Barron A, Wang Y, Korndorf T, et al. Altered Dermal Fibroblasts in Systemic Sclerosis Display Podoplanin and CD90. Am J Pathol. 2016;186:2650-64 pubmed publisher
immunocytochemistry; mouse; 1:10; loading ...; tbl 2	Li Y, Adomat H, Guns E, Hojabrpour P, Duronio V, Curran T, et al. Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor. J Cell Physiol. 2016;231:1350-63 pubmed publisher
western blot; human	Lavieu G, Zheng H, Rothman J. Stapled Golgi cisternae remain in place as cargo passes through the stack. elife. 2013;2:e00558 pubmed publisher
western blot; mouse	Cieslik K, Trial J, Carlson S, Taffet G, Entman M. Aberrant differentiation of fibroblast progenitors contributes to fibrosis in the aged murine heart: role of elevated circulating insulin levels. FASEB J. 2013;27:1761-71 pubmed publisher
	Jalili R, Pourghadiri A, Li Y, Cleversey C, Kilani R, Ghahary A. Split Thickness Grafts Grow From Bottom Up in Large Skin Injuries. J Burn Care Res. 2019;40:727-733 pubmed publisher
	Peck B, Brightwell C, Johnson D, Ireland M, Noehren B, Fry C. Anterior Cruciate Ligament Tear Promotes Skeletal Muscle Myostatin Expression, Fibrogenic Cell Expansion, and a Decline in Muscle Quality. Am J Sports Med. 2019;47:1385-1395 pubmed publisher
	McCaughey J, Stevenson N, Cross S, Stephens D. ER-to-Golgi trafficking of procollagen in the absence of large carriers. J Cell Biol. 2019;218:929-948 pubmed publisher
	Scheiber A, Guess A, Kaito T, Abzug J, Enomoto Iwamoto M, Leikin S, et al. Endoplasmic reticulum stress is induced in growth plate hypertrophic chondrocytes in G610C mouse model of osteogenesis imperfecta. Biochem Biophys Res Commun. 2019;509:235-240 pubmed publisher
	Forrester A, De Leonibus C, Grumati P, Fasana E, Piemontese M, Staiano L, et al. A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complex. EMBO J. 2019;38: pubmed publisher
	Shin J, Choi H, Yang S, Choi J, Na J, Huh C, et al. The increase of interfollicular epidermal stem cells and regulation of aryl hydrocarbon receptor and its repressors in the skin through hydroporation with anti-aging cocktail. J Cosmet Dermatol. 2019;18:1133-1139 pubmed publisher
	Gerber T, Murawala P, Knapp D, Masselink W, Schuez M, Hermann S, et al. Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science. 2018;362: pubmed publisher
	Wong M, Doan N, DiChiara A, Papa L, Cheah J, Soule C, et al. A High-Throughput Assay for Collagen Secretion Suggests an Unanticipated Role for Hsp90 in Collagen Production. Biochemistry. 2018;57:2814-2827 pubmed publisher
	Amirhosseini M, Madsen R, Escott K, Bostrom M, Ross F, Fahlgren A. GSK-3β inhibition suppresses instability-induced osteolysis by a dual action on osteoblast and osteoclast differentiation. J Cell Physiol. 2018;233:2398-2408 pubmed publisher
	Koo J, Nam M, Jung Y, Yoo J, Kim D, Kim G, et al. Anti-fibrogenic effect of PPAR-Î³ agonists in human intestinal myofibroblasts. BMC Gastroenterol. 2017;17:73 pubmed publisher
	Gorur A, Yuan L, Kenny S, Baba S, Xu K, Schekman R. COPII-coated membranes function as transport carriers of intracellular procollagen I. J Cell Biol. 2017;216:1745-1759 pubmed publisher
	Li Y, Jalili R, Ghahary A. Accelerating skin wound healing by M-CSF through generating SSEA-1 and -3 stem cells in the injured sites. Sci Rep. 2016;6:28979 pubmed publisher
	Kim E, Kim Y, Kim M, Kim S, Kim J, Lee D, et al. UV-induced inhibition of adipokine production in subcutaneous fat aggravates dermal matrix degradation in human skin. Sci Rep. 2016;6:25616 pubmed publisher
	Viti F, Landini M, Mezzelani A, Petecchia L, Milanesi L, Scaglione S. Osteogenic Differentiation of MSC through Calcium Signaling Activation: Transcriptomics and Functional Analysis. PLoS ONE. 2016;11:e0148173 pubmed publisher
	Lavieu G, Dunlop M, Lerich A, Zheng H, Bottanelli F, Rothman J. The Golgi ribbon structure facilitates anterograde transport of large cargoes. Mol Biol Cell. 2014;25:3028-36 pubmed publisher
	Kim S, Lee H, Jung S, Kim S, Lee J, Nam S, et al. Role of secreted type I collagen derived from stromal cells in two breast cancer cell lines. Oncol Lett. 2014;8:507-512 pubmed
	Desai V, Hsia H, Schwarzbauer J. Reversible modulation of myofibroblast differentiation in adipose-derived mesenchymal stem cells. PLoS ONE. 2014;9:e86865 pubmed publisher
	González Rosa J, Peralta M, Mercader N. Pan-epicardial lineage tracing reveals that epicardium derived cells give rise to myofibroblasts and perivascular cells during zebrafish heart regeneration. Dev Biol. 2012;370:173-86 pubmed publisher
	Ferrari A, Petterino C, Ratto A, Campanella C, Würth R, Thellung S, et al. CXCR4 expression in feline mammary carcinoma cells: evidence of a proliferative role for the SDF-1/CXCR4 axis. BMC Vet Res. 2012;8:27 pubmed publisher
	Santiago J, Dangerfield A, Rattan S, Bathe K, Cunnington R, Raizman J, et al. Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts. Dev Dyn. 2010;239:1573-84 pubmed publisher
	Seo J, Kim S, Shin M, Kim M, Eun H, Park C, et al. Ultraviolet irradiation induces thrombospondin-1 which attenuates type I procollagen downregulation in human dermal fibroblasts. J Dermatol Sci. 2010;59:16-24 pubmed publisher
	Chan A, Jensen K, Skokos D, Doty S, Lederman H, Kaplan R, et al. Id1 represses osteoclast-dependent transcription and affects bone formation and hematopoiesis. PLoS ONE. 2009;4:e7955 pubmed publisher
	Merrett K, Fagerholm P, McLaughlin C, Dravida S, Lagali N, Shinozaki N, et al. Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: performance of type I versus type III collagen. Invest Ophthalmol Vis Sci. 2008;49:3887-94 pubmed publisher
	Provenzano P, Alejandro Osorio A, Grorud K, Martinez D, Vailas A, Grindeland R, et al. Systemic administration of IGF-I enhances healing in collagenous extracellular matrices: evaluation of loaded and unloaded ligaments. BMC Physiol. 2007;7:2 pubmed
	Nagy N, Bíró E, Takacs A, Pólos M, Magyar A, Oláh I. Peripheral blood fibrocytes contribute to the formation of the avian spleen. Dev Dyn. 2005;232:55-66 pubmed
	Gotkin M, Ripley C, Lamande S, Bateman J, Bienkowski R. Intracellular trafficking and degradation of unassociated proalpha2 chains of collagen type I. Exp Cell Res. 2004;296:307-16 pubmed
	Miyake T, Vaglia J, Taylor L, Hall B. Development of dermal denticles in skates (Chondrichthyes, Batoidea): patterning and cellular differentiation. J Morphol. 1999;241:61-81 pubmed
	Ju H, Hao J, Zhao S, Dixon I. Antiproliferative and antifibrotic effects of mimosine on adult cardiac fibroblasts. Biochim Biophys Acta. 1998;1448:51-60 pubmed
	Guldberg R, Caldwell N, Guo X, Goulet R, Hollister S, Goldstein S. Mechanical stimulation of tissue repair in the hydraulic bone chamber. J Bone Miner Res. 1997;12:1295-302 pubmed
	Aulthouse A, Alroy J. An in vitro model for abnormal skeletal development in the lysosomal storage diseases. Virchows Arch. 1995;426:135-40 pubmed
	Foellmer H, Kawahara K, Madri J, Furthmayr H, Timpl R, Tuderman L. A monoclonal antibody specific for the amino terminal cleavage site of procollagen type I. Eur J Biochem. 1983;134:183-9 pubmed
	Joos K, Sandra A. Microarterial synthetic graft repair: interstitial cellular components. Microsurgery. 1990;11:268-77 pubmed

product information

Internal ID :

3220

Name :

SP1.D8

Depositor Name :

Furthmayr, H.

Depositor Institution :

Stanford University

Date Deposited :

8/25/88

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Collagen, Type I pro-peptide

Antigen Species :

Sheep

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Avian,Bovine,Canine,Chicken,Chondrichthyes,Human,Mouse,Porcine,Rabbit,Rat,Sheep,Xenopus,Zebrafish

Species Tested (legacy) :

chick, calf, canine, human neck, rat and zebrafish

Initial Publication Pubmed ID :

6190652

Depositor Notes (Special Instructions) :

This antibody does not cross react with Type III collagen propeptide. This recognize pro-collagen Type I in intracellular vesicles prior to collagen N-terminal pro-peptide cleavage. This antibody does not stain the ECM.

Collections :

Extracellular matrix proteins,Human,Xenopus

Search Keywords :

Furthmayr, H., Collagen, Type I pro-peptide, Sheep, MIgG1, Sheep/Human/Chicken/Bovine/Rabbit/Canine/Rat/Chondrichthyes/Zebrafish/Mouse/Xenopus/Avian/Porcine , COL1A1, OI4, AB_528438, Monoclonal, Human/Xenopus/Extracellular matrix proteins, Western blot/Immunofluorescence/Immunohistochemistry/ELISA/FFPE

Antigen Molecular Weight :

138.9 kDa

Gene :

COL1A1

Alternate Gene Name(s) :

OI4

Uniprot ID :

P02452

Antibody Registry ID :

AB_528438

Immunogen :

CNBr cleavage of pro-collagen type I from dermatosparactice sheep

Immunogen Sequence :

Amino propeptide amino acids 1-9

Clonality :

Monoclonal

Myeloma Strain :

SP2/0

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal residues in Type I collagen

Epitope Map PubMed ID :

6190652

Recommended Applications :

ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

2255244 6190652 9258761 15580570 18515574 26828589 30381873 30579604 30995070

Immunofluorescence Pubmed IDs :

7757283 6190652 9824667 10398324 20503355 22877945 31314104

Western Blot Pubmed IDs :

27161953 20494556 9824667 6190652 20503355

Immunoprecipitation Pubmed IDs :

15149860

ELISA Pubmed IDs :

20503355

FFPE Pubmed IDs :

20503355

Additional Information :

The N-terminal propeptide extends from a.a 23-161.

DSHB Growth Medium :

RPMI

References (legacy) :

Eur. J. Biochem. 134, 183-189.; Microsurgery 11, 268-277.; J. Tiss. Cult. Meth. 14, 31-36.; Virchows Archiv 426, 135-140.; J. Bone Miner. Res. 12(8), 1295-1302.; Biochim. Biophys. Acta. 1448, 51-60.; J. Morphol. 241, 61-81.; Dev. Dyn. 232, 55-66.; Dev. Dyn. 239, 1573-1584.; Invest. Ophthalmol. Vis. Sci. 49(9), 3887-3894.

more info or order :

Developmental Studies Hybridoma Bank product webpage