Myosin Heavy Chain Type IIA | Developmental Studies Hybridoma Bank SC-71 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin Heavy Chain Type IIA

catalog :

SC-71

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SC-71

reactivity :

human, mouse, brown rat, dogs, bovine, swine

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 115

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3f	Owen A, Patel S, Smith J, Balasuriya B, Mori S, Hawk G, et al. Chronic muscle weakness and mitochondrial dysfunction in the absence of sustained atrophy in a preclinical sepsis model. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 4a	Gallot Y, Bohnert K, Straughn A, Xiong G, Hindi S, Kumar A. PERK regulates skeletal muscle mass and contractile function in adult mice. FASEB J. 2019;33:1946-1962 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 3b western blot; mouse; loading ...; fig 3c	Koh J, Hancock C, Terada S, Higashida K, Holloszy J, Han D. PPARÎ² Is Essential for Maintaining Normal Levels of PGC-1Î± and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise. Cell Metab. 2017;25:1176-1185.e5 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 4a	Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep. 2017;5: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 1g	Lee C, Hanna A, Wang H, Dagnino Acosta A, Joshi A, Knoblauch M, et al. A chemical chaperone improves muscle function in mice with a RyR1 mutation. Nat Commun. 2017;8:14659 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 3b	Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3c	Morrow R, Picard M, Derbeneva O, Leipzig J, McManus M, Gouspillou G, et al. Mitochondrial energy deficiency leads to hyperproliferation of skeletal muscle mitochondria and enhanced insulin sensitivity. Proc Natl Acad Sci U S A. 2017;114:2705-2710 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 1f	Cortez Toledo O, Schnair C, Sangngern P, Metzger D, Chao L. Nur77 deletion impairs muscle growth during developmental myogenesis and muscle regeneration in mice. PLoS ONE. 2017;12:e0171268 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 3	Shen C, Zhou J, Wang X, Yu X, Liang C, Liu B, et al. Angiotensin-II-induced Muscle Wasting is Mediated by 25-Hydroxycholesterol via GSK3? Signaling Pathway. EBioMedicine. 2017;16:238-250 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 5a	Hogarth M, Houweling P, Thomas K, Gordish Dressman H, Bello L, Pegoraro E, et al. Evidence for ACTN3 as a genetic modifier of Duchenne muscular dystrophy. Nat Commun. 2017;8:14143 pubmed publisher
immunohistochemistry; human; 1:200; loading ...	St Jean Pelletier F, Pion C, Leduc Gaudet J, Sgarioto N, Zovilé I, Barbat Artigas S, et al. The impact of ageing, physical activity, and pre-frailty on skeletal muscle phenotype, mitochondrial content, and intramyocellular lipids in men. J Cachexia Sarcopenia Muscle. 2017;8:213-228 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 9a	Peake J, Roberts L, Figueiredo V, Egner I, Krog S, Aas S, et al. The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise. J Physiol. 2017;595:695-711 pubmed publisher
immunohistochemistry; mouse; 1:2	Southard S, Kim J, Low S, Tsika R, Lepper C. Myofiber-specific TEAD1 overexpression drives satellite cell hyperplasia and counters pathological effects of dystrophin deficiency. elife. 2016;5: pubmed publisher
immunohistochemistry; human; loading ...; fig 1a	White S, McDermott M, Sufit R, Kosmac K, Bugg A, Gonzalez Freire M, et al. Walking performance is positively correlated to calf muscle fiber size in peripheral artery disease subjects, but fibers show aberrant mitophagy: an observational study. J Transl Med. 2016;14:284 pubmed publisher
immunohistochemistry - frozen section; human; 1:200; loading ...; fig 4a	Spendiff S, Vuda M, Gouspillou G, Aare S, Pérez A, Morais J, et al. Denervation drives mitochondrial dysfunction in skeletal muscle of octogenarians. J Physiol. 2016;594:7361-7379 pubmed publisher
immunohistochemistry - frozen section; brown rat; 1:200; fig 1	Aare S, Spendiff S, Vuda M, Elkrief D, Pérez A, Wu Q, et al. Failed reinnervation in aging skeletal muscle. Skelet Muscle. 2016;6:29 pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 4a	Woodall B, Woodall M, Luongo T, Grisanti L, Tilley D, Elrod J, et al. Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy. J Biol Chem. 2016;291:21913-21924 pubmed
immunohistochemistry; brown rat; 1:100; loading ...; fig 1d	Rui Y, Pan F, Mi J. Composition of Muscle Fiber Types in Rat Rotator Cuff Muscles. Anat Rec (Hoboken). 2016;299:1397-401 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 5	Riaz M, Raz Y, van Putten M, Paniagua Soriano G, Krom Y, Florea B, et al. PABPN1-Dependent mRNA Processing Induces Muscle Wasting. PLoS Genet. 2016;12:e1006031 pubmed publisher
immunohistochemistry; mouse; fig 2	Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
immunohistochemistry - frozen section; brown rat; loading ...	Pannérec A, Springer M, Migliavacca E, Ireland A, Piasecki M, Karaz S, et al. A robust neuromuscular system protects rat and human skeletal muscle from sarcopenia. Aging (Albany NY). 2016;8:712-29 pubmed publisher
immunohistochemistry; human; 1:200; loading ...; tbl 2	Nyberg M, Fiorenza M, Lund A, Christensen M, RÃ¸mer T, Piil P, et al. Adaptations to Speed Endurance Training in Highly Trained Soccer Players. Med Sci Sports Exerc. 2016;48:1355-64 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; fig 1	Nagahisa H, Okabe K, Iuchi Y, Fujii J, Miyata H. Characteristics of Skeletal Muscle Fibers of SOD1 Knockout Mice. Oxid Med Cell Longev. 2016;2016:9345970 pubmed publisher
immunohistochemistry - frozen section; human; loading ...; fig 7a	Arentson Lantz E, English K, Paddon Jones D, Fry C. Fourteen days of bed rest induces a decline in satellite cell content and robust atrophy of skeletal muscle fibers in middle-aged adults. J Appl Physiol (1985). 2016;120:965-75 pubmed publisher
immunohistochemistry; mouse; fig 4	Foltz S, Modi J, Melick G, Abousaud M, Luan J, Fortunato M, et al. Abnormal Skeletal Muscle Regeneration plus Mild Alterations in Mature Fiber Type Specification in Fktn-Deficient Dystroglycanopathy Muscular Dystrophy Mice. PLoS ONE. 2016;11:e0147049 pubmed publisher
immunohistochemistry - frozen section; human; 1:200; fig 1b	Power G, Minozzo F, Spendiff S, Filion M, Konokhova Y, Purves Smith M, et al. Reduction in single muscle fiber rate of force development with aging is not attenuated in world class older masters athletes. Am J Physiol Cell Physiol. 2016;310:C318-27 pubmed publisher
immunohistochemistry; mouse; fig 1	Ebert S, Dyle M, Bullard S, Dierdorff J, Murry D, Fox D, et al. Identification and Small Molecule Inhibition of an Activating Transcription Factor 4 (ATF4)-dependent Pathway to Age-related Skeletal Muscle Weakness and Atrophy. J Biol Chem. 2015;290:25497-511 pubmed publisher
immunohistochemistry; mouse; fig s5	Ohsawa Y, Takayama K, Nishimatsu S, Okada T, Fujino M, Fukai Y, et al. The Inhibitory Core of the Myostatin Prodomain: Its Interaction with Both Type I and II Membrane Receptors, and Potential to Treat Muscle Atrophy. PLoS ONE. 2015;10:e0133713 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 3	Li F, Buck D, De Winter J, Kolb J, Meng H, Birch C, et al. Nebulin deficiency in adult muscle causes sarcomere defects and muscle-type-dependent changes in trophicity: novel insights in nemaline myopathy. Hum Mol Genet. 2015;24:5219-33 pubmed publisher
immunohistochemistry; human	Walton R, Finlin B, Mula J, Long D, Zhu B, Fry C, et al. Insulin-resistant subjects have normal angiogenic response to aerobic exercise training in skeletal muscle, but not in adipose tissue. Physiol Rep. 2015;3: pubmed publisher
immunohistochemistry - frozen section; mouse; fig 5	Tian L, Ding S, You Y, Li T, Liu Y, Wu X, et al. Leiomodin-3-deficient mice display nemaline myopathy with fast-myofiber atrophy. Dis Model Mech. 2015;8:635-41 pubmed publisher
immunocytochemistry; mouse	Fajardo V, Bombardier E, McMillan E, TRAN K, Wadsworth B, Gamu D, et al. Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype. Dis Model Mech. 2015;8:999-1009 pubmed publisher
immunohistochemistry - frozen section; swine; 1:200	Clark D, Clark D, Beever J, Dilger A. Increased prenatal IGF2 expression due to the porcine intron3-G3072A mutation may be responsible for increased muscle mass. J Anim Sci. 2015;93:2546-58 pubmed publisher
immunohistochemistry; human; fig 1	Mitchell C, Oikawa S, Ogborn D, Nates N, MacNeil L, Tarnopolsky M, et al. Daily chocolate milk consumption does not enhance the effect of resistance training in young and old men: a randomized controlled trial. Appl Physiol Nutr Metab. 2015;40:199-202 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 9	Seaberg B, Henslee G, Wang S, Paez Colasante X, Landreth G, Rimer M. Muscle-derived extracellular signal-regulated kinases 1 and 2 are required for the maintenance of adult myofibers and their neuromuscular junctions. Mol Cell Biol. 2015;35:1238-53 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 2	Tontonoz P, Cortez Toledo O, Wroblewski K, Hong C, Lim L, Carranza R, et al. The orphan nuclear receptor Nur77 is a determinant of myofiber size and muscle mass in mice. Mol Cell Biol. 2015;35:1125-38 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100	Fry C, Lee J, Mula J, Kirby T, Jackson J, Liu F, et al. Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. Nat Med. 2015;21:76-80 pubmed publisher
immunohistochemistry; brown rat; 1:40	Mori T, Agata N, Itoh Y, Miyazu Inoue M, Sokabe M, Taguchi T, et al. Stretch speed-dependent myofiber damage and functional deficits in rat skeletal muscle induced by lengthening contraction. Physiol Rep. 2014;2: pubmed publisher
immunohistochemistry; human; 1:500	Skovgaard C, Christensen P, Larsen S, Andersen T, Thomassen M, Bangsbo J. Concurrent speed endurance and resistance training improves performance, running economy, and muscle NHE1 in moderately trained runners. J Appl Physiol (1985). 2014;117:1097-109 pubmed publisher
immunohistochemistry; mouse; 1:200 immunohistochemistry; human; 1:200 immunohistochemistry; brown rat; 1:200	Gouspillou G, Sgarioto N, Norris B, Barbat Artigas S, Aubertin Leheudre M, Morais J, et al. The relationship between muscle fiber type-specific PGC-1Î± content and mitochondrial content varies between rodent models and humans. PLoS ONE. 2014;9:e103044 pubmed publisher
immunohistochemistry; human; 1:500	Andersen T, Schmidt J, Thomassen M, Hornstrup T, Frandsen U, Randers M, et al. A preliminary study: effects of football training on glucose control, body composition, and performance in men with type 2 diabetes. Scand J Med Sci Sports. 2014;24 Suppl 1:43-56 pubmed publisher
immunohistochemistry; human	Fry C, Noehren B, Mula J, Ubele M, Westgate P, Kern P, et al. Fibre type-specific satellite cell response to aerobic training in sedentary adults. J Physiol. 2014;592:2625-35 pubmed publisher
immunohistochemistry - frozen section; mouse	Sousa Victor P, Gutarra S, García Prat L, Rodriguez Ubreva J, Ortet L, Ruiz Bonilla V, et al. Geriatric muscle stem cells switch reversible quiescence into senescence. Nature. 2014;506:316-21 pubmed publisher
immunohistochemistry - frozen section; human; 1:200	Gouspillou G, Sgarioto N, Kapchinsky S, Purves Smith F, Norris B, Pion C, et al. Increased sensitivity to mitochondrial permeability transition and myonuclear translocation of endonuclease G in atrophied muscle of physically active older humans. FASEB J. 2014;28:1621-33 pubmed publisher
immunohistochemistry; mouse; fig 3	Garton F, Seto J, Quinlan K, Yang N, Houweling P, North K. ?-Actinin-3 deficiency alters muscle adaptation in response to denervation and immobilization. Hum Mol Genet. 2014;23:1879-93 pubmed publisher
immunohistochemistry - frozen section; mouse	Barton E, Park S, James J, Makarewich C, Philippou A, Eletto D, et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J. 2012;26:3691-702 pubmed publisher
	Carroll K, Bazyler C, Bernards J, Taber C, Stuart C, DeWeese B, et al. Skeletal Muscle Fiber Adaptations Following Resistance Training Using Repetition Maximums or Relative Intensity. Sports (Basel). 2019;7: pubmed publisher
	Sonjak V, Jacob K, Morais J, Rivera Zengotita M, Spendiff S, Spake C, et al. Fidelity of muscle fibre reinnervation modulates ageing muscle impact in elderly women. J Physiol. 2019;597:5009-5023 pubmed publisher
	Hunt L, Stover J, Haugen B, Shaw T, Li Y, Pagala V, et al. A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice. Cell Rep. 2019;28:1268-1281.e6 pubmed publisher
	Suzuki J. Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice. Physiol Rep. 2019;7:e14182 pubmed publisher
	Liu Z, Chen J, Chen Z. Muscle Fiber Type Changes in Lumbrical Muscles at Early Stages of Chronic Nerve Compression. Curr Med Sci. 2019;39:59-66 pubmed publisher
	Iso T, Haruyama H, Sunaga H, Matsui M, Matsui H, Tanaka R, et al. Exercise endurance capacity is markedly reduced due to impaired energy homeostasis during prolonged fasting in FABP4/5 deficient mice. BMC Physiol. 2019;19:1 pubmed publisher
	Sellers R, Mahmood S, Perumal G, Macaluso F, Kurland I. Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic ( fld) Mice. Vet Pathol. 2019;56:322-331 pubmed publisher
	Ichikawa H, Matsuo T, Higurashi Y, Nagahisa H, Miyata H, Sugiura T, et al. Characteristics of Muscle Fiber-Type Distribution in Moles. Anat Rec (Hoboken). 2019;302:1010-1023 pubmed publisher
	Yao X, Yu T, Xi F, Xu Y, Ma L, Pan X, et al. BAMBI shuttling between cytosol and membrane is required for skeletal muscle development and regeneration. Biochem Biophys Res Commun. 2018;: pubmed publisher
	Cullins M, Krekeler B, Connor N. Differential impact of tongue exercise on intrinsic lingual muscles. Laryngoscope. 2018;128:2245-2251 pubmed publisher
	Yalvac M, Amornvit J, Braganza C, Chen L, Hussain S, Shontz K, et al. Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis. Skelet Muscle. 2017;7:27 pubmed publisher
	Greschik H, Duteil D, Messaddeq N, Willmann D, Arrigoni L, Sum M, et al. The histone code reader Spin1 controls skeletal muscle development. Cell Death Dis. 2017;8:e3173 pubmed publisher
	Murgia M, Toniolo L, Nagaraj N, Ciciliot S, Vindigni V, Schiaffino S, et al. Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging. Cell Rep. 2017;19:2396-2409 pubmed publisher
	Saha M, Mitsuhashi S, Jones M, Manko K, Reddy H, Bruels C, et al. Consequences of MEGF10 deficiency on myoblast function and Notch1 interactions. Hum Mol Genet. 2017;26:2984-3000 pubmed publisher
	Greising S, Vasdev A, Zhan W, Sieck G, Mantilla C. Chronic TrkB agonist treatment in old age does not mitigate diaphragm neuromuscular dysfunction. Physiol Rep. 2017;5: pubmed publisher
	Jarosch I, Gehlert S, Jacko D, Koczulla R, Wencker M, Welte T, et al. Different Training-Induced Skeletal Muscle Adaptations in COPD Patients with and without Alpha-1 Antitrypsin Deficiency. Respiration. 2016;92:339-347 pubmed
	Sakakibara I, Wurmser M, Dos Santos M, Santolini M, Ducommun S, Davaze R, et al. Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle. Skelet Muscle. 2016;6:30 pubmed publisher
	De Smet S, Van Thienen R, Deldicque L, James R, Sale C, Bishop D, et al. Nitrate Intake Promotes Shift in Muscle Fiber Type Composition during Sprint Interval Training in Hypoxia. Front Physiol. 2016;7:233 pubmed publisher
	Pardes A, Freedman B, Fryhofer G, Salka N, Bhatt P, Soslowsky L. Males have Inferior Achilles Tendon Material Properties Compared to Females in a Rodent Model. Ann Biomed Eng. 2016;44:2901-2910 pubmed publisher
	Shin J, Nunomiya A, Kitajima Y, Dan T, Miyata T, Nagatomi R. Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway. Skelet Muscle. 2016;6:5 pubmed publisher
	Bergmeister K, GrÃ¶ger M, Aman M, Willensdorfer A, Manzano Szalai K, Salminger S, et al. Automated muscle fiber type population analysis with ImageJ of whole rat muscles using rapid myosin heavy chain immunohistochemistry. Muscle Nerve. 2016;54:292-9 pubmed publisher
	Gorski J, Huffman N, Vallejo J, Brotto L, Chittur S, Breggia A, et al. Deletion of Mbtps1 (Pcsk8, S1p, Ski-1) Gene in Osteocytes Stimulates Soleus Muscle Regeneration and Increased Size and Contractile Force with Age. J Biol Chem. 2016;291:4308-22 pubmed publisher
	Stark D, Coffey N, Pancoast H, Arnold L, Walker J, VallÃ©e J, et al. Ephrin-A3 promotes and maintains slow muscle fiber identity during postnatal development and reinnervation. J Cell Biol. 2015;211:1077-91 pubmed publisher
	Hyatt H, Toedebusch R, Ruegsegger G, Mobley C, Fox C, McGinnis G, et al. Comparative adaptations in oxidative and glycolytic muscle fibers in a low voluntary wheel running rat model performing three levels of physical activity. Physiol Rep. 2015;3: pubmed publisher
	Seiler S, Koves T, Gooding J, Wong K, Stevens R, Ilkayeva O, et al. Carnitine Acetyltransferase Mitigates Metabolic Inertia and Muscle Fatigue during Exercise. Cell Metab. 2015;22:65-76 pubmed publisher
	Greising S, Medina MartÃnez J, Vasdev A, Sieck G, Mantilla C. Analysis of muscle fiber clustering in the diaphragm muscle of sarcopenic mice. Muscle Nerve. 2015;52:76-82 pubmed publisher
	Arbour D, Tremblay E, Martineau Ã, Julien J, Robitaille R. Early and persistent abnormal decoding by glial cells at the neuromuscular junction in an ALS model. J Neurosci. 2015;35:688-706 pubmed publisher
	Ballak S, Jaspers R, Deldicque L, Chalil S, Peters E, de Haan A, et al. Blunted hypertrophic response in old mouse muscle is associated with a lower satellite cell density and is not alleviated by resveratrol. Exp Gerontol. 2015;62:23-31 pubmed publisher
	Breda A, Pereira de Albuquerque A, Jardim C, Morinaga L, Suesada M, Fernandes C, et al. Skeletal muscle abnormalities in pulmonary arterial hypertension. PLoS ONE. 2014;9:e114101 pubmed publisher
	Ballak S, Degens H, BusÃ© Pot T, de Haan A, Jaspers R. Plantaris muscle weakness in old mice: relative contributions of changes in specific force, muscle mass, myofiber cross-sectional area, and number. Age (Dordr). 2014;36:9726 pubmed publisher
	Talmadge R, Acosta W, Garland T. Myosin heavy chain isoform expression in adult and juvenile mini-muscle mice bred for high-voluntary wheel running. Mech Dev. 2014;134:16-30 pubmed publisher
	Li Z, Parlakian A, Coletti D, Alonso Martin S, HourdÃ© C, Joanne P, et al. Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy. J Cell Sci. 2014;127:4589-601 pubmed publisher
	Kopecka K, Zacharová G, Smerdu V, Soukup T. Slow to fast muscle transformation following heterochronous isotransplantation is influenced by host thyroid hormone status. Histochem Cell Biol. 2014;142:677-84 pubmed publisher
	Pal R, Palmieri M, Loehr J, Li S, Abo Zahrah R, Monroe T, et al. Src-dependent impairment of autophagy by oxidative stress in a mouse model of Duchenne muscular dystrophy. Nat Commun. 2014;5:4425 pubmed publisher
	Moore C, Crocker D, Fahlman A, Moore M, Willoughby D, Robbins K, et al. Ontogenetic changes in skeletal muscle fiber type, fiber diameter and myoglobin concentration in the Northern elephant seal (Mirounga angustirostris). Front Physiol. 2014;5:217 pubmed publisher
	Scribbans T, Edgett B, Vorobej K, Mitchell A, Joanisse S, Matusiak J, et al. Fibre-specific responses to endurance and low volume high intensity interval training: striking similarities in acute and chronic adaptation. PLoS ONE. 2014;9:e98119 pubmed publisher
	Sakakibara I, Santolini M, Ferry A, Hakim V, Maire P. Six homeoproteins and a Iinc-RNA at the fast MYH locus lock fast myofiber terminal phenotype. PLoS Genet. 2014;10:e1004386 pubmed publisher
	Zhang P, Li W, Liu H, Li J, Wang J, Li Y, et al. Dystrophin involved in the susceptibility of slow muscles to hindlimb unloading via concomitant activation of TGF-?1/Smad3 signaling and ubiquitin-proteasome degradation in mice. Cell Biochem Biophys. 2014;70:1057-67 pubmed publisher
	Alexander M, Casar J, Motohashi N, Vieira N, Eisenberg I, Marshall J, et al. MicroRNA-486-dependent modulation of DOCK3/PTEN/AKT signaling pathways improves muscular dystrophy-associated symptoms. J Clin Invest. 2014;124:2651-67 pubmed publisher
	Lawlor M, Viola M, Meng H, Edelstein R, Liu F, Yan K, et al. Differential muscle hypertrophy is associated with satellite cell numbers and Akt pathway activation following activin type IIB receptor inhibition in Mtm1 p.R69C mice. Am J Pathol. 2014;184:1831-42 pubmed publisher
	Malfatti E, Lehtokari V, Böhm J, De Winter J, Schäffer U, Estournet B, et al. Muscle histopathology in nebulin-related nemaline myopathy: ultrastrastructural findings correlated to disease severity and genotype. Acta Neuropathol Commun. 2014;2:44 pubmed publisher
	Dyle M, Ebert S, Cook D, Kunkel S, Fox D, Bongers K, et al. Systems-based discovery of tomatidine as a natural small molecule inhibitor of skeletal muscle atrophy. J Biol Chem. 2014;289:14913-24 pubmed publisher
	Sitparan P, Pagel C, Pinniger G, Yoo H, Mackie E, Bakker A. Contractile properties of slow and fast skeletal muscles from protease activated receptor-1 null mice. Muscle Nerve. 2014;50:991-8 pubmed publisher
	Dyar K, Ciciliot S, Wright L, Biensø R, Tagliazucchi G, Patel V, et al. Muscle insulin sensitivity and glucose metabolism are controlled by the intrinsic muscle clock. Mol Metab. 2014;3:29-41 pubmed publisher
	Bruells C, Bergs I, Rossaint R, Du J, Bleilevens C, Goetzenich A, et al. Recovery of diaphragm function following mechanical ventilation in a rodent model. PLoS ONE. 2014;9:e87460 pubmed publisher
	Fajardo V, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, et al. Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers. PLoS ONE. 2013;8:e84304 pubmed publisher
	Bennett B, Mohamed J, Alway S. Effects of resveratrol on the recovery of muscle mass following disuse in the plantaris muscle of aged rats. PLoS ONE. 2013;8:e83518 pubmed publisher
	Gangopadhyay S. Systemic administration of follistatin288 increases muscle mass and reduces fat accumulation in mice. Sci Rep. 2013;3:2441 pubmed publisher
	Luo Q, Douglas M, BURKHOLDER T, Sokoloff A. Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles. Muscle Nerve. 2014;49:534-44 pubmed publisher
	Storbeck C, Al Zahrani K, Sriram R, Kawesa S, O Reilly P, Daniel K, et al. Distinct roles for Ste20-like kinase SLK in muscle function and regeneration. Skelet Muscle. 2013;3:16 pubmed publisher
	Gan Z, Rumsey J, Hazen B, Lai L, Leone T, Vega R, et al. Nuclear receptor/microRNA circuitry links muscle fiber type to energy metabolism. J Clin Invest. 2013;123:2564-75 pubmed publisher
	Lessard S, Rivas D, Alves Wagner A, Hirshman M, Gallagher I, Constantin Teodosiu D, et al. Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks. Diabetes. 2013;62:2717-27 pubmed publisher
	Huguet A, Medja F, Nicole A, Vignaud A, Guiraud Dogan C, Ferry A, et al. Molecular, physiological, and motor performance defects in DMSXL mice carrying >1,000 CTG repeats from the human DM1 locus. PLoS Genet. 2012;8:e1003043 pubmed publisher
	Kawai M, Aida H, Hiraga A, Miyata H. Muscle satellite cells are activated after exercise to exhaustion in Thoroughbred horses. Equine Vet J. 2013;45:512-7 pubmed publisher
	Srikuea R, Symons T, Long D, Lee J, Shang Y, Chomentowski P, et al. Association of fibromyalgia with altered skeletal muscle characteristics which may contribute to postexertional fatigue in postmenopausal women. Arthritis Rheum. 2013;65:519-28 pubmed publisher
	Didier N, Hourde C, Amthor H, Marazzi G, Sassoon D. Loss of a single allele for Ku80 leads to progenitor dysfunction and accelerated aging in skeletal muscle. EMBO Mol Med. 2012;4:910-23 pubmed publisher
	Braun T, Orwoll B, Zhu X, Levasseur P, Szumowski M, Nguyen M, et al. Regulation of lean mass, bone mass, and exercise tolerance by the central melanocortin system. PLoS ONE. 2012;7:e42183 pubmed publisher
	Kontro H, Hulmi J, Rahkila P, Kainulainen H. Cellular and tissue expression of DAPIT, a phylogenetically conserved peptide. Eur J Histochem. 2012;56:e18 pubmed publisher
	Goodman C, Kotecki J, Jacobs B, Hornberger T. Muscle fiber type-dependent differences in the regulation of protein synthesis. PLoS ONE. 2012;7:e37890 pubmed publisher
	Joanne P, Hourde C, Ochala J, Caudéran Y, Medja F, Vignaud A, et al. Impaired adaptive response to mechanical overloading in dystrophic skeletal muscle. PLoS ONE. 2012;7:e35346 pubmed publisher
	Valdez G, Tapia J, Lichtman J, Fox M, Sanes J. Shared resistance to aging and ALS in neuromuscular junctions of specific muscles. PLoS ONE. 2012;7:e34640 pubmed publisher
	Chai R, Vukovic J, Dunlop S, Grounds M, Shavlakadze T. Striking denervation of neuromuscular junctions without lumbar motoneuron loss in geriatric mouse muscle. PLoS ONE. 2011;6:e28090 pubmed publisher
	Braun T, Zhu X, Szumowski M, Scott G, Grossberg A, Levasseur P, et al. Central nervous system inflammation induces muscle atrophy via activation of the hypothalamic-pituitary-adrenal axis. J Exp Med. 2011;208:2449-63 pubmed publisher
	De Repentigny Y, Ferrier A, Ryan S, Sato T, Kothary R. Motor unit abnormalities in Dystonia musculorum mice. PLoS ONE. 2011;6:e21093 pubmed publisher
	Rhee H, Steel C, Derksen F, Robinson N, Hoh J. Immunohistochemical analysis of laryngeal muscles in normal horses and horses with subclinical recurrent laryngeal neuropathy. J Histochem Cytochem. 2009;57:787-800 pubmed publisher
	Acevedo L, Rivero J. New insights into skeletal muscle fibre types in the dog with particular focus towards hybrid myosin phenotypes. Cell Tissue Res. 2006;323:283-303 pubmed
	Sharp S, Dingermann T, Schaack J, Sharp J, Burke D, DeRobertis E, et al. Each element of the Drosophila tRNAArg gene split promoter directs transcription in Xenopus oocytes. Nucleic Acids Res. 1983;11:8677-90 pubmed
	Magwood S. Vaccination against infectious bovine rhinotracheitis may hamper export trade in breeding cattle. Can Vet J. 1974;15:260 pubmed
	Schiaffino S, Gorza L, Sartore S, Saggin L, Ausoni S, Vianello M, et al. Three myosin heavy chain isoforms in type 2 skeletal muscle fibres. J Muscle Res Cell Motil. 1989;10:197-205 pubmed

product information

Internal ID :

5829

Name :

SC-71

Depositor Name :

Schiaffino, S.

Depositor Institution :

University of Padova

Date Deposited :

3/25/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin Heavy Chain Type IIA

Antigen Species :

Bovine

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Canine,Goat,Horse,Human,Mole,Mouse,Porcine,Rabbit,Rat,Sheep

Species Tested (legacy) :

rat, mouse, horse, guinea pig, bovine, llama, goat, pig, sheep and human

Initial Publication Pubmed ID :

2547831

Depositor Notes (Special Instructions) :

This antibody recognizes the fast twitch isoform MyHC IIA specifically in horses and rodents. SC-71 does recognize both IIA and 2X in human and canine muscle (PMID: 18840559 and 22530000).

Collections :

Human,Skeletal muscle

Search Keywords :

Schiaffino, Stefano, Myosin Heavy Chain Type IIA, Myosin heavy chain 2A, Bovine, MIgG1, Rat/Mouse/Human/Horse/Canine/Porcine/Bovine, MYH2, MyHC-2a, AB_2147165, monoclonal

Antigen Molecular Weight :

223 kD Predicted

Gene :

MYH2

Alternate Gene Name(s) :

MyHC-2a

Uniprot ID :

Q9BE41

Antibody Registry ID :

AB_2147165

Synonyms (Alternate Clone Names) :

SC-71

Immunogen :

Purified myosin isolated from subcutaneous muscle

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS0

Epitope Mapped :

Yes

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

25179606 24358354 23835800 25217814 25028342 24349525 23942549 23815977 23676496 23124535 23209425 22848742 22629468 22485182 22084407 21698255 2547831 19398607 16163488 25028342 31365869 24692104 25028342 24726641 4371120 25413330 24719321 25605336 24839113 24903461 25414077 24852826 25121500 24959151 25589763 6561520 30381013 27686000 27687713 26719336 31365869 31368533

Immunofluorescence Pubmed IDs :

21698255 23206314 24789910 24567902 6561520 27150673 28498977 29168801 29243257 29241457 26788932 27150673 30376699 30580997 30866899 30868492 31373325 28614723 31328438

Western Blot Pubmed IDs :

24358354 23815977 2547831 25217814 24959151 31373325

Pubmed IDs :

25562814 22688299

Additional Information :

This antibody has also tested positive on elephant seal, guinea pig, llama and goat muscle.

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Muscle Res. Cell. Motil. 10(3), 197-205.; Cell Tissue Res. 323, 283-303. ; J. Biol. Chem. 288(2), 1226-1237.; J. Histochem & Cytochem. 57(8), 787-800.

more info or order :

Developmental Studies Hybridoma Bank product webpage