synaptobrevin-1 | Developmental Studies Hybridoma Bank SB1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

synaptobrevin-1

catalog :

SB1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SB1

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Reference
Schäffer U, Baumeister R, Schulze E. Targeted purification of SnAvi-tagged proteins. Methods Mol Biol. 2014;1177:163-74 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher
Schäffer U, Schlosser A, Müller K, Schäfer A, Katava N, Baumeister R, et al. SnAvi--a new tandem tag for high-affinity protein-complex purification. Nucleic Acids Res. 2010;38:e91 pubmed publisher
Gracheva E, Hadwiger G, Nonet M, Richmond J. Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density. Neurosci Lett. 2008;444:137-42 pubmed publisher

product information

Internal ID :

3909

Name :

SB1

Depositor Name :

Nonet, M.L. / Hadwiger, G.

Depositor Institution :

Washington University Medical School

Date Deposited :

6/21/06

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

synaptobrevin-1

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans, not tested in other species

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Specific to SNB-1; by western blot, the wild type band was absent and a higher MW band of reduced intensity was observed in snb-1 mutant [md247]. Synaptobrevin is localized primarily to synaptic vesicles. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was seen in the nerve ring, pharyngeal nervous system, and the ventral and dorsal nerve cords.

Collections :

C. elegans,Cell signaling,Neurodevelopment

Search Keywords :

Nonet, M.L. / Hadwiger, G., synaptobrevin-1, C. elegans, MIgG1, C. elegans, snb-1, , AB_579792, monoclonal, C. elegans/Cell signaling/Neurodevelopment, Immunofluorescence/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 12 kDa; Apparent: 16 kDa

Gene :

snb-1

Uniprot ID :

O02495

Antibody Registry ID :

AB_579792

Synonyms (Alternate Clone Names) :

5D1

Immunogen :

Recombinant His6-tagged SNB-1 (aa 13-88) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

cytoplasmic (aa 17-26)

Epitope Map PubMed ID :

20047968

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Good

Immunofluorescence Pubmed IDs :

20405020

Western Blot Pubmed IDs :

20405020 18721860 20047968

Immunoprecipitation Pubmed IDs :

24943322

Additional Information :

SNB-1 is also known as VAMP1. SNB-1 is a neuronal v-SNARE synaptobrevin (VAMP) which is localized primarily to synaptic vesicles in C. elegans

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. A monoclonal antibody toolkit for C. elegans. PLoS One. 2010; 5(4): e10161. Published online 2010 April 13. doi: 10.1371/journal.pone.0010161. Schaeffer, U., Schlosser, A., Mueller, K.M., Schaefer, A., Katava, N., Baumeister, R., and Schulze, E. (2010). SnAvi—a new tandem tag for high-affinity protein-complex purification. Nucleic Acids Res. 2010 38: e91; doi:10.1093/nar/gkp1178.

more info or order :

Developmental Studies Hybridoma Bank product webpage