Myosin heavy chain, slow contracting muscle | Developmental Studies Hybridoma Bank S58 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain, slow contracting muscle

catalog :

S58

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S58

reactivity :

human, mouse, rat, chicken, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Reference
Ruf Zamojski F, Trivedi V, Fraser S, Trinh L. Spatio-Temporal Differences in Dystrophin Dynamics at mRNA and Protein Levels Revealed by a Novel FlipTrap Line. PLoS ONE. 2015;10:e0128944 pubmed publisher
Nord H, Burguiere A, Muck J, Nord C, Ahlgren U, Von Hofsten J. Differential regulation of myosin heavy chains defines new muscle domains in zebrafish. Mol Biol Cell. 2014;25:1384-95 pubmed publisher
Ketley A, Warren A, Holmes E, Gering M, Aboobaker A, Brook J. The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development. PLoS ONE. 2013;8:e65170 pubmed publisher
Weltman N, Ojamaa K, Savinova O, Chen Y, Schlenker E, Zucchi R, et al. Restoration of cardiac tissue thyroid hormone status in experimental hypothyroidism: a dose-response study in female rats. Endocrinology. 2013;154:2542-52 pubmed publisher
Elworthy S, Hargrave M, Knight R, Mebus K, Ingham P. Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development. 2008;135:2115-26 pubmed publisher
Wohlgemuth S, Crawford B, Pilgrim D. The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish. Dev Biol. 2007;303:483-92 pubmed
Putman C, Xu X, Gillies E, Maclean I, Bell G. Effects of strength, endurance and combined training on myosin heavy chain content and fibre-type distribution in humans. Eur J Appl Physiol. 2004;92:376-84 pubmed
Crow M, Stockdale F. Myosin expression and specialization among the earliest muscle fibers of the developing avian limb. Dev Biol. 1986;113:238-54 pubmed
Miller J, Crow M, Stockdale F. Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures. J Cell Biol. 1985;101:1643-50 pubmed
Page S, Miller J, DiMario J, Hager E, Moser A, Stockdale F. Developmentally regulated expression of three slow isoforms of myosin heavy chain: diversity among the first fibers to form in avian muscle. Dev Biol. 1992;154:118-28 pubmed

product information

Internal ID :

382

Name :

S58

Depositor Name :

Stockdale, F.E.

Depositor Institution :

Stanford University School of Medicine

Depositor Website :

http://med.stanford.edu/labs/frank_stockdale/

Date Deposited :

2/12/04

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain, slow contracting muscle

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgA

Isotype for catalog (legacy) :

IgA

Positive Tested Species Reactivity :

Chicken,Human,Mouse,Quail,Rat,Zebrafish

Species Tested (legacy) :

chicken, quail, zebrafish, mouse, rat, others

Initial Publication Pubmed ID :

3902852

Depositor Notes (Special Instructions) :

In birds, this antibody recognizes myosin heavy chain isoforms SM2 and SM3, but not SM1. The antibody does not recognize aldehyde-fixed tissue. Ethanol fixation is preferred. For western blotting, 5% SDS-PAGE gels are recommended.

Collections :

Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Frank E. Stockdale, Myosin heavy chain, slow contracting muscle, Chicken, MIgA, Chicken/Quail/Zebrafish/Mouse/Rat/Human, MYH7B, SM2, SSMHC, myosin-7B, AB_528377, Monoclonal, Skeletal muscle/Human, Immunohistochemistry/Immunofluorescence/Western blot

Antigen Molecular Weight :

~200 kDa

Gene :

MYH7B

Alternate Gene Name(s) :

SM2, SSMHC, myosin-7B

Uniprot ID :

E1C523

Antibody Registry ID :

AB_528377

Immunogen :

Anterior latissimus dorsi (ALD) muscle from Day 19 embryo limb

Immunogen Sequence :

Purified full length myosin

Clonality :

Monoclonal

Myeloma Strain :

P3/NS1.1Ag4-1

Epitope Mapped :

Yes

Epitope Map PubMed ID :

1426621

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

3902852 3943663 15241691 23755189 17189627

Immunofluorescence Pubmed IDs :

3902852 15241691 18480160

Western Blot Pubmed IDs :

3902852 3943663 23594789

Additional Information :

RRID:AB_528377

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 101, 1643-1650.; Dev. Biol. 113, 238-254.; Endocrinology 154(7), 2542-2552.

more info or order :

Developmental Studies Hybridoma Bank product webpage