Myosin heavy chain, slow developmental (sd-MyHC) | Developmental Studies Hybridoma Bank S46 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain, slow developmental (sd-MyHC)

catalog :

S46

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

S46

reactivity :

human, mouse, rat, chicken, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; zebrafish ; 1:10; loading ...	Witzel H, Cheedipudi S, Gao R, Stainier D, Dobreva G. Isl2b regulates anterior second heart field development in zebrafish. Sci Rep. 2017;7:41043 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:400	Komori T, Gyobu H, Ueno H, Kitamura T, Senba E, Morikawa Y. Expression of kin of irregular chiasm-like 3/mKirre in proprioceptive neurons of the dorsal root ganglia and its interaction with nephrin in muscle spindles. J Comp Neurol. 2008;511:92-108 pubmed publisher
	Zhu D, Fang Y, Gao K, Shen J, Zhong T, Li F. Vegfa Impacts Early Myocardium Development in Zebrafish. Int J Mol Sci. 2017;18: pubmed publisher
	Vaughan S, Stanley O, Valdez G. Impact of Aging on Proprioceptive Sensory Neurons and Intrafusal Muscle Fibers in Mice. J Gerontol A Biol Sci Med Sci. 2017;72:771-779 pubmed publisher
	Li H, Zhong Y, Wang Z, Gao J, Xu J, Chu W, et al. Smyd1b is required for skeletal and cardiac muscle function in zebrafish. Mol Biol Cell. 2013;24:3511-21 pubmed publisher
	Sokoloff A, Li H, Burkholder T. Limited expression of slow tonic myosin heavy chain in human cranial muscles. Muscle Nerve. 2007;36:183-9 pubmed
	Wohlgemuth S, Crawford B, Pilgrim D. The myosin co-chaperone UNC-45 is required for skeletal and cardiac muscle function in zebrafish. Dev Biol. 2007;303:483-92 pubmed
	Berdougo E, Coleman H, Lee D, Stainier D, Yelon D. Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish. Development. 2003;130:6121-9 pubmed
	Miller J, Crow M, Stockdale F. Slow and fast myosin heavy chain content defines three types of myotubes in early muscle cell cultures. J Cell Biol. 1985;101:1643-50 pubmed
	Stainier D, Fishman M. Patterning the zebrafish heart tube: acquisition of anteroposterior polarity. Dev Biol. 1992;153:91-101 pubmed

product information

Internal ID :

376

Name :

S46

Depositor Name :

Stockdale, F.E.

Depositor Institution :

Stanford University School of Medicine

Depositor Website :

http://med.stanford.edu/labs/frank_stockdale/

Date Deposited :

2/12/04

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain, slow developmental (sd-MyHC)

Antigen Species :

Chicken

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Chicken,Human,Mouse,Quail,Rat,Zebrafish

Species Tested (legacy) :

chicken, quail, mouse, rat, zebrafish

Initial Publication Pubmed ID :

3902852

Depositor Notes (Special Instructions) :

This antigen is also known as myosin heavy chain, cardiac muscle alpha isoform. S46 preferentially stains slow tonic myosin heavy chain in humans including extraocular, intrafusal and the atrium of the heart.

Collections :

Cytoskeleton,Skeletal muscle

Search Keywords :

Frank E. Stockdale, Myosin heavy chain, slow developmental (sd-MyHC), Chicken , MIgG1, kappa light chain, Chicken/Quail/Mouse/Rat/Zebrafish/Human, MYH6, MYHCA, CMY6, AB_528376, Monoclonal, Human/Skeletal muscle, Immunohistochemistry/Immunofluorescence/Western blot

Antigen Molecular Weight :

223.7 kDa

Gene :

MYH6

Alternate Gene Name(s) :

MYHCA, CMY6

Uniprot ID :

P13533

Antibody Registry ID :

AB_528376

Immunogen :

Purified myosin from Pectoralis major

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

P3/NS1.1Ag4-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

Mab S46 recognizes an epitope of atrium-specific myosin heavy, Myh6.

Epitope Map PubMed ID :

1516755 14573521

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

17486578 3902852 17189627

Immunofluorescence Pubmed IDs :

14573521 3902852 1516755 27688482

Western Blot Pubmed IDs :

17486578 3902852

Additional Information :

RRID:AB_528376)

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 101, 1643-1650.; Muscle Nerve 36, 183-189.; J. Comp. Neurol. 511, 92-108.

more info or order :

Developmental Studies Hybridoma Bank product webpage