product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Rop Protein/ Ras opposite protein
catalog :
Rop 4F8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Rop 4F8
reactivity :
fruit fly
application :
western blot, immunohistochemistry, immunocytochemistry
citations: 10
Published Application/Species/Sample/DilutionReference
  • western blot; fruit fly; 1:10
Koppen T, Weckmann A, Muller S, Staubach S, Bloch W, Dohmen R, et al. Proteomics analyses of microvesicles released by Drosophila Kc167 and S2 cells. Proteomics. 2011;11:4397-410 pubmed publisher
Reddy Alla S, Böhme M, Reynolds E, Beis C, Grasskamp A, Mampell M, et al. Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission. Neuron. 2017;95:1350-1364.e12 pubmed publisher
Gordon D, Chia J, Jayawardena K, Antrobus R, Bard F, Peden A. VAMP3/Syb and YKT6 are required for the fusion of constitutive secretory carriers with the plasma membrane. PLoS Genet. 2017;13:e1006698 pubmed publisher
Debruhl H, Albertson R, Swider Z, Sullivan W. Rop, the Sec1/Munc18 homolog in Drosophila, is required for furrow ingression and stable cell shape during cytokinesis. J Cell Sci. 2016;129:430-43 pubmed publisher
Peng Y, Lee J, Rowland K, Wen Y, Hua H, Carlson N, et al. Regulation of dendrite growth and maintenance by exocytosis. J Cell Sci. 2015;128:4279-92 pubmed publisher
Ng F, Jackson F. The ROP vesicle release factor is required in adult Drosophila glia for normal circadian behavior. Front Cell Neurosci. 2015;9:256 pubmed publisher
Khodosh R, Augsburger A, Schwarz T, Garrity P. Bchs, a BEACH domain protein, antagonizes Rab11 in synapse morphogenesis and other developmental events. Development. 2006;133:4655-65 pubmed
Verstreken P, Koh T, Schulze K, Zhai R, Hiesinger P, Zhou Y, et al. Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. Neuron. 2003;40:733-48 pubmed
Wu M, Schulze K, Lloyd T, Bellen H. The ROP-syntaxin interaction inhibits neurotransmitter release. Eur J Cell Biol. 2001;80:196-9 pubmed
Harrison S, Broadie K, van de Goor J, Rubin G. Mutations in the Drosophila Rop gene suggest a function in general secretion and synaptic transmission. Neuron. 1994;13:555-66 pubmed
product information
Internal ID :
1927
Name :
Rop 4F8
Depositor Name :
Rubin, G.M.
Depositor Institution :
University of California at Berkeley
Depositor Website :
https://www.janelia.org/lab/rubin-lab
Date Deposited :
8/2/06
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Rop Protein/ Ras opposite protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
7917291
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Rubin, G.M., Rop Protein/ Ras opposite protein, Drosophila , MIgG1, kappa light chain, Drosophila , Rop, Dmel_CG15811 , AB_1157869, Monoclonal, Drosophila antigens, Western blot/Immunohistochemistry/Immunofluorescence
Antigen Molecular Weight :
67.8 kDa
Gene :
Rop
Alternate Gene Name(s) :
Dmel_CG15811
Uniprot ID :
Q07327
Antibody Registry ID :
AB_1157869
Immunogen :
Recombinant GST-Rop fusion protein
Immunogen Sequence :
a.a. 180-389
Clonality :
Monoclonal
Myeloma Strain :
P3XAg8.653
Epitope Mapped :
No
Recommended Applications :
Immunofluorescence,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Yes
Immunohistochemistry Pubmed IDs :
7917291
Immunofluorescence Pubmed IDs :
26190976 26631487 26483382 28403141
Western Blot Pubmed IDs :
7917291 28403141 11302525 14622578 17079274 28403141 21901833 28867551
Additional Information :
RRID:AB_1157869. FBgn0004574.
DSHB Growth Medium :
Iscove's
References (legacy) :
Neuron 13, 555-566.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US