neurofilament (NF-H) | Developmental Studies Hybridoma Bank RT97 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

neurofilament (NF-H)

catalog :

RT97

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RT97

reactivity :

human, mouse, rat, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry, FACS, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 38

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:2000; loading ...	Maggio D, Singh A, Iorgulescu J, Bleicher D, Ghosh M, Lopez M, et al. Identifying the Long-Term Role of Inducible Nitric Oxide Synthase after Contusive Spinal Cord Injury Using a Transgenic Mouse Model. Int J Mol Sci. 2017;18: pubmed publisher
immunohistochemistry - paraffin section; human; 1:800; loading ...	van der Zee J, Gijselinck I, Van Mossevelde S, Perrone F, Dillen L, Heeman B, et al. TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis. Hum Mutat. 2017;38:297-309 pubmed publisher
immunohistochemistry; rat; 1:50	Gaillard F, Kuny S, Sauve Y. Retinal distribution of Disabled-1 in a diurnal murine rodent, the Nile grass rat Arvicanthis niloticus. Exp Eye Res. 2014;125:236-43 pubmed publisher
immunohistochemistry - frozen section; rat; 1:2000	Tripathi R, McTigue D. Chronically increased ciliary neurotrophic factor and fibroblast growth factor-2 expression after spinal contusion in rats. J Comp Neurol. 2008;510:129-44 pubmed publisher
	Church J, Milich L, Lerch J, Popovich P, McTigue D. E6020, a synthetic TLR4 agonist, accelerates myelin debris clearance, Schwann cell infiltration, and remyelination in the rat spinal cord. Glia. 2017;65:883-899 pubmed publisher
	Santos D, Gonzalez Perez F, Giudetti G, Micera S, Udina E, del Valle J, et al. Preferential Enhancement of Sensory and Motor Axon Regeneration by Combining Extracellular Matrix Components with Neurotrophic Factors. Int J Mol Sci. 2016;18: pubmed publisher
	Harwell C, Coleman M. Synaptophysin depletion and intraneuronal A? in organotypic hippocampal slice cultures from huAPP transgenic mice. Mol Neurodegener. 2016;11:44 pubmed publisher
	De Groef L, Andries L, Lemmens K, Van Hove I, Moons L. Matrix metalloproteinases in the mouse retina: a comparative study of expression patterns and MMP antibodies. BMC Ophthalmol. 2015;15:187 pubmed publisher
	Kim K, Son K, Palmore G. Neuron Image Analyzer: Automated and Accurate Extraction of Neuronal Data from Low Quality Images. Sci Rep. 2015;5:17062 pubmed publisher
	Kastriti M, Sargiannidou I, Kleopa K, Karagogeos D. Differential modulation of the juxtaparanodal complex in Multiple Sclerosis. Mol Cell Neurosci. 2015;67:93-103 pubmed publisher
	Tsai Y, Lu B, Bakondi B, Girman S, Sahabian A, Sareen D, et al. Human iPSC-Derived Neural Progenitors Preserve Vision in an AMD-Like Model. Stem Cells. 2015;33:2537-49 pubmed publisher
	Solin S, Wang Y, Mauldin J, Schultz L, Lincow D, Brodskiy P, et al. Molecular and cellular characterization of a zebrafish optic pathway tumor line implicates glia-derived progenitors in tumorigenesis. PLoS ONE. 2014;9:e114888 pubmed publisher
	Mihailovska E, Raith M, Valencia R, Fischer I, Al Banchaabouchi M, Herbst R, et al. Neuromuscular synapse integrity requires linkage of acetylcholine receptors to postsynaptic intermediate filament networks via rapsyn-plectin 1f complexes. Mol Biol Cell. 2014;25:4130-49 pubmed publisher
	García Ayuso D, Salinas Navarro M, Nadal Nicolás F, Ortín Martínez A, Agudo Barriuso M, Vidal Sanz M, et al. Sectorial loss of retinal ganglion cells in inherited photoreceptor degeneration is due to RGC death. Br J Ophthalmol. 2014;98:396-401 pubmed publisher
	Viehöfer A, Shinohara Y, Sprecher C, Boszczyk B, Buettner A, Benjamin M, et al. The molecular composition of the extracellular matrix of the human iliolumbar ligament. Spine J. 2015;15:1325-31 pubmed publisher
	Barbour H, Plant C, Harvey A, Plant G. Tissue sparing, behavioral recovery, supraspinal axonal sparing/regeneration following sub-acute glial transplantation in a model of spinal cord contusion. BMC Neurosci. 2013;14:106 pubmed publisher
	Maden M, Manwell L, Ormerod B. Proliferation zones in the axolotl brain and regeneration of the telencephalon. Neural Dev. 2013;8:1 pubmed publisher
	Marei H, Ahmed A, Michetti F, Pescatori M, Pallini R, Casalbore P, et al. Gene expression profile of adult human olfactory bulb and embryonic neural stem cell suggests distinct signaling pathways and epigenetic control. PLoS ONE. 2012;7:e33542 pubmed publisher
	García Ayuso D, Salinas Navarro M, Agudo Barriuso M, Alarcón Martínez L, Vidal Sanz M, Villegas Pérez M. Retinal ganglion cell axonal compression by retinal vessels in light-induced retinal degeneration. Mol Vis. 2011;17:1716-33 pubmed
	De Repentigny Y, Ferrier A, Ryan S, Sato T, Kothary R. Motor unit abnormalities in Dystonia musculorum mice. PLoS ONE. 2011;6:e21093 pubmed publisher
	Albersen M, Fandel T, Zhang H, Banie L, Lin G, De Ridder D, et al. Pentoxifylline promotes recovery of erectile function in a rat model of postprostatectomy erectile dysfunction. Eur Urol. 2011;59:286-96 pubmed publisher
	Salinas Navarro M, Alarcón Martínez L, Valiente Soriano F, Ortín Martínez A, Jiménez López M, Avilés Trigueros M, et al. Functional and morphological effects of laser-induced ocular hypertension in retinas of adult albino Swiss mice. Mol Vis. 2009;15:2578-98 pubmed
	Kuny S, Gaillard F, Mema S, Freund P, Zhang K, Macdonald I, et al. Inner retina remodeling in a mouse model of stargardt-like macular dystrophy (STGD3). Invest Ophthalmol Vis Sci. 2010;51:2248-62 pubmed publisher
	Casalbore P, Budoni M, Ricci Vitiani L, Cenciarelli C, Petrucci G, Milazzo L, et al. Tumorigenic potential of olfactory bulb-derived human adult neural stem cells associates with activation of TERT and NOTCH1. PLoS ONE. 2009;4:e4434 pubmed publisher
	Veeranna -, Lee J, Pareek T, Jaffee H, Boland B, Vinod K, et al. Neurofilament tail phosphorylation: identity of the RT-97 phosphoepitope and regulation in neurons by cross-talk among proline-directed kinases. J Neurochem. 2008;107:35-49 pubmed publisher
	Gaillard F, Bonfield S, Gilmour G, Kuny S, Mema S, Martin B, et al. Retinal anatomy and visual performance in a diurnal cone-rich laboratory rodent, the Nile grass rat (Arvicanthis niloticus). J Comp Neurol. 2008;510:525-38 pubmed publisher
	Jiang S, Bendjelloul F, Ballerini P, D Alimonte I, Nargi E, Jiang C, et al. Guanosine reduces apoptosis and inflammation associated with restoration of function in rats with acute spinal cord injury. Purinergic Signal. 2007;3:411-21 pubmed publisher
	Marco Gomariz M, Hurtado Montalbán N, Vidal Sanz M, Lund R, Villegas Pérez M. Phototoxic-induced photoreceptor degeneration causes retinal ganglion cell degeneration in pigmented rats. J Comp Neurol. 2006;498:163-79 pubmed
	Bampton E, Taylor J. Effects of Schwann cell secreted factors on PC12 cell neuritogenesis and survival. J Neurobiol. 2005;63:29-48 pubmed
	Hwang I, DO S, Yoo K, Kim D, Cho J, Kwon Y, et al. Chronological alterations of neurofilament 150 immunoreactivity in the gerbil hippocampus and dentate gyrus after transient forebrain ischemia. Brain Res. 2004;1016:119-28 pubmed
	Young H, Black A. Adult stem cells. Anat Rec A Discov Mol Cell Evol Biol. 2004;276:75-102 pubmed
	Young H. Existence of reserve quiescent stem cells in adults, from amphibians to humans. Curr Top Microbiol Immunol. 2004;280:71-109 pubmed
	Young H, Duplaa C, Young T, Floyd J, Reeves M, Davis K, et al. Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells. Anat Rec. 2001;263:350-60 pubmed
	Walro J, Wang J, Story G. Afferent-inherent regulation of myosin heavy chain isoforms in rat muscle spindles. Muscle Nerve. 1997;20:1549-60 pubmed
	Murphy A, Breen K, Long A, Feighery C, Casey E, Kelleher D. Neurofilament expression in human T lymphocytes. Immunology. 1993;79:167-70 pubmed
	Wood J, Anderton B. Monoclonal antibodies to mammalian neurofilaments. Biosci Rep. 1981;1:263-8 pubmed
	Anderton B, Breinburg D, Downes M, Green P, Tomlinson B, Ulrich J, et al. Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants. Nature. 1982;298:84-6 pubmed
	Miller C, Brion J, Calvert R, Chin T, Eagles P, Downes M, et al. Alzheimer's paired helical filaments share epitopes with neurofilament side arms. EMBO J. 1986;5:269-76 pubmed

product information

Internal ID :

1156

Name :

RT97

Depositor Name :

Wood, J.

Depositor Institution :

Sandoz Institute for Medical Research

Date Deposited :

7/30/87

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

neurofilament (NF-H)

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Bovine,Chicken,Human,Mouse,Rat

Species Tested (legacy) :

human, chicken, rat

Initial Publication Pubmed ID :

7028152

Depositor Notes (Special Instructions) :

The antibody strongly recognizes polyphosphorylated epitopes on neurofilaments (and to a much lesser extent, on other molecules, e. g. rhodopsin and histones).

Collections :

Cell markers,Cytoskeleton,Human,Neurodevelopment,Stem cell

Search Keywords :

Wood, J., neurofilament (NF-H), Rat, MIgG1, Human/Chicken/Rat/Bovine/Mouse, NEFH, KIAA0845, NFH, AB_528399, monoclonal, Cytoskeletal elements/Human/Stem cell/Cell markers/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/FACS

Antigen Molecular Weight :

Predicted: 112 kDa; Apparent: 200 kDa

Gene :

NEFH

Alternate Gene Name(s) :

KIAA0845, NFH

Uniprot ID :

F1ND22

Antibody Registry ID :

AB_528399

Immunogen :

Semi-purified neurofilaments from rat brain homogenate.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

X63.Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

Phospho-epitopes on the tail domain of neurofilament heavy (and to a lesser extent, neurofilament medium) at KSPXK or KSPXXXK motifs.

Epitope Map PubMed ID :

18715269

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

6178036 24139866 14699636 14594208 9390668 11500811 8509137 28251686

Immunofluorescence Pubmed IDs :

15702477 18680202 19933199 21036464 18715269 25869002 26070930 26714639 31386177 31421150

Western Blot Pubmed IDs :

6178036 15702477 18715269 2423324 15234260 27287430 8509137

FACS Pubmed IDs :

8509137

Additional Information :

RT97 reacts with neurofibrillary tangles and plaques [PMID 6178036]. RT97 cross-reacts with neurofilament medium. RT97 has been used as a cell marker for neurons.

DSHB Growth Medium :

Iscove's

References (legacy) :

Biosci. Rep. 1, 263-268.; Nature 298, 84-86.; Anat. Rec. 249, 32-43.; Muscle Nerve 20, 1549-1560.; Anat. Rec. 263, 350-360.; Curr. Top. Microbiol. Immunol. 280, 71-109.; Anat. Rec. Part A 276A, 75-102.; J. Neurobiol. 63, 29-48.; J. Comp. Neurol. 510, 525-538.; Invest. Ophthalmol. Vis. Sci. 51(4), 2248-2262.; Eur. Urol. doi:10.1016/j.eururo.2010.10.034 In press

more info or order :

Developmental Studies Hybridoma Bank product webpage