RME-1 | Developmental Studies Hybridoma Bank RME1 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

RME-1

catalog :

RME1

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RME1

reactivity :

Caenorhabditis elegans

application :

western blot, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Huynh J, Dang H, Munoz Tucker I, O Ketch M, Liu I, Perno S, et al. ESCRT-Dependent Cell Death in a Caenorhabditis elegans Model of the Lysosomal Storage Disorder Mucolipidosis Type IV. Genetics. 2016;202:619-38 pubmed publisher
Gamerdinger M, Hanebuth M, Frickey T, Deuerling E. The principle of antagonism ensures protein targeting specificity at the endoplasmic reticulum. Science. 2015;348:201-7 pubmed publisher
Zhang P, Na H, Liu Z, Zhang S, Xue P, Chen Y, et al. Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets. Mol Cell Proteomics. 2012;11:317-28 pubmed publisher
Cheng L, Li B, Wang Z. Level-Set Minimization of Potential Controlled Hadwiger Valuations for Molecular Solvation. J Comput Phys. 2010;229:8497-8510 pubmed
Chen B, Jiang Y, Zeng S, Yan J, Li X, Zhang Y, et al. Endocytic sorting and recycling require membrane phosphatidylserine asymmetry maintained by TAT-1/CHAT-1. PLoS Genet. 2010;6:e1001235 pubmed publisher
Hadwiger G, Dour S, Arur S, Fox P, Nonet M. A monoclonal antibody toolkit for C. elegans. PLoS ONE. 2010;5:e10161 pubmed publisher

product information

Internal ID :

6518

Name :

RME1

Depositor Name :

Nonet, M.L. / Hadwiger, G. / Dour, S.

Depositor Institution :

Washington University Medical School

Date Deposited :

2/16/10

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

RME-1

Antigen Species :

C. elegans

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

C. elegans

Species Tested (legacy) :

C. elegans

Initial Publication Pubmed ID :

20405020

Depositor Notes (Special Instructions) :

Works on C. elegans whole mounts. Stains recycling endosomes.

Collections :

C. elegans

Search Keywords :

Nonet, M.L. / Hadwiger, G. / Dour, S., RME-1, C. elegans, MIgG1, C. elegans, rme-1, , AB_10571460, monoclonal, C. elegans, Immunofluorescence/Western Blot

Antigen Molecular Weight :

Predicted: multiple isoforms from 63 to 94 kDa (65 kDa for isoform d); Apparent: 70 kDa

Gene :

rme-1

Uniprot ID :

G5EFP1

Antibody Registry ID :

AB_10571460

Synonyms (Alternate Clone Names) :

5G11

Immunogen :

Recombinant His6-tagged RME-1 (C-terminal 243 aa of isoform d) fusion protein

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

P3X63Ag8.653

Epitope Mapped :

Yes

Epitope Location or Sequence :

C-terminal 243 aa of isoform d

Epitope Map PubMed ID :

20405020

Recommended Applications :

Immunofluorescence,Western Blot

Immunoblotting (legacy) :

good

Immunofluorescence Pubmed IDs :

20405020 21170358

Western Blot Pubmed IDs :

20405020 25859040 26596346 22493183

Additional Information :

RME-1 is an evolutionarily conserved protein specifically associated with recycling endosomes. The Uniprot ID provided is for isoform d (Receptor-mediated endocytosis 1 isoform IV).

DSHB Growth Medium :

Iscove's

References (legacy) :

Hadwiger, G., Dour, S., Arur, S., Fox, P., and Nonet, M.L. (2010) A monoclonal antibody toolkit for C. elegans. PLoS One. 5(4):e10161-10115.

more info or order :

Developmental Studies Hybridoma Bank product webpage