Nestin/radial glial marker | Developmental Studies Hybridoma Bank RC2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Nestin/radial glial marker

catalog :

RC2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RC2

reactivity :

mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 52

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:20; loading ...; fig 1s2b1c	S nchez Guardado L, Lois C. Lineage does not regulate the sensory synaptic input of projection neurons in the mouse olfactory bulb. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:5; loading ...; fig 1c	Lee M, Sitko A, Khalid S, Shirasu Hiza M, Mason C. Spatiotemporal distribution of glia in and around the developing mouse optic tract. J Comp Neurol. 2019;527:508-521 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:10; loading ...; fig 6c	Laporte M, Chatellard C, Vauchez V, Hemming F, Deloulme J, Vossier F, et al. Alix is required during development for normal growth of the mouse brain. Sci Rep. 2017;7:44767 pubmed publisher
	Nakagawa N, Li J, Yabuno Nakagawa K, Eom T, Cowles M, Mapp T, et al. APC sets the Wnt tone necessary for cerebral cortical progenitor development. Genes Dev. 2017;31:1679-1692 pubmed publisher
	Bruce F, Brown S, Smith J, Fuerst P, Erskine L. DSCAM promotes axon fasciculation and growth in the developing optic pathway. Proc Natl Acad Sci U S A. 2017;114:1702-1707 pubmed publisher
	Nechiporuk T, MCGANN J, Mullendorff K, Hsieh J, Wurst W, Floss T, et al. The REST remodeling complex protects genomic integrity during embryonic neurogenesis. elife. 2016;5:e09584 pubmed publisher
	Wang L, Wang J, Ma D, Taylor J, Chan S. Isoform-specific localization of Nogo protein in the optic pathway of mouse embryos. J Comp Neurol. 2016;524:2322-34 pubmed publisher
	Laumonnerie C, Tong Y, Alstermark H, Wilson S. Commissural axonal corridors instruct neuronal migration in the mouse spinal cord. Nat Commun. 2015;6:7028 pubmed publisher
	Li K, Leung A, Guo Q, Yang W, Li J. Shp2-dependent ERK signaling is essential for induction of Bergmann glia and foliation of the cerebellum. J Neurosci. 2014;34:922-31 pubmed publisher
	Zhang J, Neal J, Lian G, Hu J, Lu J, Sheen V. Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation. J Neurosci. 2013;33:15735-46 pubmed publisher
	Liu N, Wang M, Deng W, Schmidt C, Qin W, Leonhardt H, et al. Intrinsic and extrinsic connections of Tet3 dioxygenase with CXXC zinc finger modules. PLoS ONE. 2013;8:e62755 pubmed publisher
	Chaubey S, Wolfe J. Transplantation of CD15-enriched murine neural stem cells increases total engraftment and shifts differentiation toward the oligodendrocyte lineage. Stem Cells Transl Med. 2013;2:444-54 pubmed publisher
	Roe M, McDonald N, Durrant B, Jensen T. Xenogeneic transfer of adult quail (Coturnix coturnix) spermatogonial stem cells to embryonic chicken (Gallus gallus) hosts: a model for avian conservation. Biol Reprod. 2013;88:129 pubmed publisher
	Wang C, Liang C, Bian Z, Zhu Y, Guan J. FIP200 is required for maintenance and differentiation of postnatal neural stem cells. Nat Neurosci. 2013;16:532-42 pubmed publisher
	Nigro A, Menon R, Bergamaschi A, Clovis Y, Baldi A, Ehrmann M, et al. MiR-30e and miR-181d control radial glia cell proliferation via HtrA1 modulation. Cell Death Dis. 2012;3:e360 pubmed publisher
	Shimada I, LeComte M, Granger J, Quinlan N, Spees J. Self-renewal and differentiation of reactive astrocyte-derived neural stem/progenitor cells isolated from the cortical peri-infarct area after stroke. J Neurosci. 2012;32:7926-40 pubmed publisher
	Ritch J, Valencia A, Alexander J, Sapp E, Gatune L, Sangrey G, et al. Multiple phenotypes in Huntington disease mouse neural stem cells. Mol Cell Neurosci. 2012;50:70-81 pubmed publisher
	Bizzoca A, Corsi P, Polizzi A, Pinto M, Xenaki D, Furley A, et al. F3/Contactin acts as a modulator of neurogenesis during cerebral cortex development. Dev Biol. 2012;365:133-51 pubmed publisher
	Velagapudi C, Nilsson R, Lee M, Burns H, Ricono J, Arar M, et al. Reciprocal induction of simple organogenesis by mouse kidney progenitor cells in three-dimensional co-culture. Am J Pathol. 2012;180:819-30 pubmed publisher
	Watanabe K, Takebayashi H, Bepari A, Esumi S, Yanagawa Y, Tamamaki N. Dpy19l1, a multi-transmembrane protein, regulates the radial migration of glutamatergic neurons in the developing cerebral cortex. Development. 2011;138:4979-90 pubmed publisher
	Milenkovic V, Röhrl E, Weber B, Strauss O. Disease-associated missense mutations in bestrophin-1 affect cellular trafficking and anion conductance. J Cell Sci. 2011;124:2988-96 pubmed publisher
	Nowakowski T, Mysiak K, Pratt T, Price D. Functional dicer is necessary for appropriate specification of radial glia during early development of mouse telencephalon. PLoS ONE. 2011;6:e23013 pubmed publisher
	Modarresi F, Faghihi M, Patel N, Sahagan B, Wahlestedt C, Lopez Toledano M. Knockdown of BACE1-AS Nonprotein-Coding Transcript Modulates Beta-Amyloid-Related Hippocampal Neurogenesis. Int J Alzheimers Dis. 2011;2011:929042 pubmed publisher
	Erskine L, Reijntjes S, Pratt T, Denti L, Schwarz Q, Vieira J, et al. VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm. Neuron. 2011;70:951-65 pubmed publisher
	Chatzi C, Brade T, Duester G. Retinoic acid functions as a key GABAergic differentiation signal in the basal ganglia. PLoS Biol. 2011;9:e1000609 pubmed publisher
	Bupesh M, Legaz I, Abellan A, Medina L. Multiple telencephalic and extratelencephalic embryonic domains contribute neurons to the medial extended amygdala. J Comp Neurol. 2011;519:1505-25 pubmed publisher
	Hu H, Li J, Gagen C, Gray N, Zhang Z, Qi Y, et al. Conditional knockout of protein O-mannosyltransferase 2 reveals tissue-specific roles of O-mannosyl glycosylation in brain development. J Comp Neurol. 2011;519:1320-37 pubmed publisher
	Lin H, Bell E, Uwanogho D, Perfect L, Noristani H, Bates T, et al. Neuronatin promotes neural lineage in ESCs via Ca(2+) signaling. Stem Cells. 2010;28:1950-60 pubmed publisher
	Miyata T, Ono Y, Okamoto M, Masaoka M, Sakakibara A, Kawaguchi A, et al. Migration, early axonogenesis, and Reelin-dependent layer-forming behavior of early/posterior-born Purkinje cells in the developing mouse lateral cerebellum. Neural Dev. 2010;5:23 pubmed publisher
	Gontan C, Guttler T, Engelen E, Demmers J, Fornerod M, Grosveld F, et al. Exportin 4 mediates a novel nuclear import pathway for Sox family transcription factors. J Cell Biol. 2009;185:27-34 pubmed publisher
	Lake B, Sokol S. Strabismus regulates asymmetric cell divisions and cell fate determination in the mouse brain. J Cell Biol. 2009;185:59-66 pubmed publisher
	Soma M, Aizawa H, Ito Y, Maekawa M, Osumi N, Nakahira E, et al. Development of the mouse amygdala as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation. J Comp Neurol. 2009;513:113-28 pubmed publisher
	Qiu R, Wang X, Davy A, Wu C, Murai K, Zhang H, et al. Regulation of neural progenitor cell state by ephrin-B. J Cell Biol. 2008;181:973-83 pubmed publisher
	Voss A, Britto J, Dixon M, Sheikh B, Collin C, Tan S, et al. C3G regulates cortical neuron migration, preplate splitting and radial glial cell attachment. Development. 2008;135:2139-49 pubmed publisher
	Strathmann F, Wang X, Mayer Proschel M. Identification of two novel glial-restricted cell populations in the embryonic telencephalon arising from unique origins. BMC Dev Biol. 2007;7:33 pubmed
	Hu H, Yang Y, Eade A, Xiong Y, Qi Y. Breaches of the pial basement membrane and disappearance of the glia limitans during development underlie the cortical lamination defect in the mouse model of muscle-eye-brain disease. J Comp Neurol. 2007;501:168-83 pubmed
	Lowell S, Benchoua A, Heavey B, Smith A. Notch promotes neural lineage entry by pluripotent embryonic stem cells. PLoS Biol. 2006;4:e121 pubmed
	Aguado T, Palazuelos J, Monory K, Stella N, Cravatt B, Lutz B, et al. The endocannabinoid system promotes astroglial differentiation by acting on neural progenitor cells. J Neurosci. 2006;26:1551-61 pubmed
	Kolk S, Whitman M, Yun M, Shete P, Donoghue M. A unique subpopulation of Tbr1-expressing deep layer neurons in the developing cerebral cortex. Mol Cell Neurosci. 2005;30:538-51 pubmed
	Niewmierzycka A, Mills J, St Arnaud R, Dedhar S, Reichardt L. Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly. J Neurosci. 2005;25:7022-31 pubmed
	Teng J, Takei Y, Harada A, Nakata T, Chen J, Hirokawa N. Synergistic effects of MAP2 and MAP1B knockout in neuronal migration, dendritic outgrowth, and microtubule organization. J Cell Biol. 2001;155:65-76 pubmed
	Skogh C, Eriksson C, Kokaia M, Meijer X, Wahlberg L, Wictorin K, et al. Generation of regionally specified neurons in expanded glial cultures derived from the mouse and human lateral ganglionic eminence. Mol Cell Neurosci. 2001;17:811-20 pubmed
	Nicot A, Dicicco Bloom E. Regulation of neuroblast mitosis is determined by PACAP receptor isoform expression. Proc Natl Acad Sci U S A. 2001;98:4758-63 pubmed
	Li Y, Baker N. Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye. Curr Biol. 2001;11:330-8 pubmed
	Hartfuss E, Galli R, Heins N, Gotz M. Characterization of CNS precursor subtypes and radial glia. Dev Biol. 2001;229:15-30 pubmed
	Hajihosseini M, Dickson C. A subset of fibroblast growth factors (Fgfs) promote survival, but Fgf-8b specifically promotes astroglial differentiation of rat cortical precursor cells. Mol Cell Neurosci. 1999;14:468-85 pubmed
	Bertuzzi S, Hindges R, Mui S, O Leary D, Lemke G. The homeodomain protein vax1 is required for axon guidance and major tract formation in the developing forebrain. Genes Dev. 1999;13:3092-105 pubmed
	Whittemore S, Morassutti D, Walters W, Liu R, Magnuson D. Mitogen and substrate differentially affect the lineage restriction of adult rat subventricular zone neural precursor cell populations. Exp Cell Res. 1999;252:75-95 pubmed
	Toresson H, Mata De Urquiza A, Fagerstrom C, Perlmann T, Campbell K. Retinoids are produced by glia in the lateral ganglionic eminence and regulate striatal neuron differentiation. Development. 1999;126:1317-26 pubmed
	Marcus R, Mason C. The first retinal axon growth in the mouse optic chiasm: axon patterning and the cellular environment. J Neurosci. 1995;15:6389-402 pubmed
	Pippard C, Roddie I. Comparison of fluid transport systems in lymphatics and veins. Lymphology. 1987;20:224-9 pubmed
	Misson J, Edwards M, Yamamoto M, Caviness V. Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker. Brain Res Dev Brain Res. 1988;44:95-108 pubmed

product information

Internal ID :

1180

Name :

RC2

Depositor Name :

Yamamoto, M.

Depositor Institution :

University of Tsukuba

Date Deposited :

2/2/96

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Nestin/radial glial marker

Antigen Species :

Rat

Host Species :

mouse

Isotype :

MIgM, lambda light chain

Isotype for catalog (legacy) :

IgM, lambda light chain

Positive Tested Species Reactivity :

Mouse

Species Tested (legacy) :

mouse, does not react with rat

Initial Publication Pubmed ID :

3069243

Depositor Notes (Special Instructions) :

This antibody recognizes the intermediate filament nestin and is a marker for pluripotent neural progenitor cells.

Collections :

Cell markers,Cytoskeleton,Neurodevelopment,Stem cell

Search Keywords :

Yamamoto, Miyuki, Nestin/radial glial marker , Rat, MIgM, lambda light chain, Mouse, Nes, , AB_531887, monoclonal, astrocytes

Antigen Molecular Weight :

295 kDa Apparent

Gene :

Nes

Uniprot ID :

Q6P5H2

Antibody Registry ID :

AB_531887

Immunogen :

Fetal brain cell lysate (E14–E17)

Clonality :

Monoclonal

Myeloma Strain :

NS-1

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunohistochemistry Pubmed IDs :

21452208 24431450 22854828 22508027 22360968 3444362 17206611 19107806 22138298 21878505 23690950 11358480 11296303 11133151 10656254 10502401 11267869 24089482 22674268 22028030 18506028 16452678 16188453 16049178 11358480 10021349 7472403 3069243

Immunofluorescence Pubmed IDs :

21452199 23575150 23681951 25960414 26718118

Western Blot Pubmed IDs :

21452208 21452199 17206611 19107806 21785702

FFPE Pubmed IDs :

10601035

Pubmed IDs :

22674268

DSHB Growth Medium :

Iscove's

References (legacy) :

Dev. Brain Res. 44(1), 95-108.; J. Neurosci. 15(10), 6389-6402.; Development 126, 1317-1326.; Mol. Cell. Neurosci. 17, 811-820.; Mol. Cell. Neurosci. 30, 538-551.; J. Neurosci. 26(5), 1551-1561.;FASEB J. 20, E1773-E1779.Mol. Cell Neurosci. 17, 811-820.; Development 138, 4979-4990.; J. Neurosci. 32(23), 7926-7940.

more info or order :

Developmental Studies Hybridoma Bank product webpage