Prospero protein | Developmental Studies Hybridoma Bank Prospero (MR1A) product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Prospero protein

catalog :

Prospero (MR1A)

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

Prospero (MR1A)

reactivity :

Drosophila melanogaster

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; Drosophila melanogaster; 1:4; loading ...; fig 1e	Menon K, Kulkarni V, Takemura S, Anaya M, Zinn K. Interactions between Dpr11 and DIP-γ control selection of amacrine neurons in Drosophila color vision circuits. elife. 2019;8: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:20; loading ...; fig 4s1d	Abdusselamoglu M, Eroglu E, Burkard T, Knoblich J. The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop. elife. 2019;8: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:10; loading ...; fig 1a	Curt J, Salmani B, Thor S. Anterior CNS expansion driven by brain transcription factors. elife. 2019;8: pubmed publisher
immunocytochemistry; Drosophila melanogaster; 1:50; fig 5s1a	He L, Binari R, Huang J, Falo Sanjuan J, Perrimon N. In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer. elife. 2019;8: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:30; loading ...; fig s4c	Hakes A, Otsuki L, Brand A. A newly discovered neural stem cell population is generated by the optic lobe neuroepithelium during embryogenesis in Drosophila melanogaster. Development. 2018;145: pubmed publisher
immunocytochemistry; Drosophila melanogaster; 1:100; loading ...; fig 1a	Zhou J, Edgar B, Boutros M. ATF3 acts as a rheostat to control JNK signalling during intestinal regeneration. Nat Commun. 2017;8:14289 pubmed publisher
immunohistochemistry - frozen section; Drosophila melanogaster; 1:100; fig 2	Li T, Giagtzoglou N, Eberl D, Jaiswal S, Cai T, Godt D, et al. The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals. elife. 2016;5: pubmed publisher
immunohistochemistry; Drosophila melanogaster; 1:50	Hudry B, Khadayate S, Miguel Aliaga I. The sexual identity of adult intestinal stem cells controls organ size and plasticity. Nature. 2016;530:344-8 pubmed publisher
immunohistochemistry - paraffin section; Drosophila melanogaster; 1:100; fig s1a	Patel P, Dutta D, Edgar B. Niche appropriation by Drosophila intestinal stem cell tumours. Nat Cell Biol. 2015;17:1182-92 pubmed publisher
	Chen J, Xu N, Wang C, Huang P, Huang H, Jin Z, et al. Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells. Nat Cell Biol. 2018;20:152-161 pubmed publisher
	Morrison C, Chen H, Cook T, Brown S, Treisman J. Glass promotes the differentiation of neuronal and non-neuronal cell types in the Drosophila eye. PLoS Genet. 2018;14:e1007173 pubmed publisher
	Toegel M, Azzam G, Lee E, Knapp D, Tan Y, Fa M, et al. A multiplexable TALE-based binary expression system for in vivo cellular interaction studies. Nat Commun. 2017;8:1663 pubmed publisher
	Singh S, Zeng X, Zhao J, Liu Y, Hou G, Liu H, et al. The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila. Nature. 2016;538:109-113 pubmed publisher
	Nakazawa M, Matsubara H, Matsushita Y, Watanabe M, Vo N, Yoshida H, et al. The Human Bcl-2 Family Member Bcl-rambo Localizes to Mitochondria and Induces Apoptosis and Morphological Aberrations in Drosophila. PLoS ONE. 2016;11:e0157823 pubmed publisher
	Urbach R, Jussen D, Technau G. Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila. Development. 2016;143:1290-301 pubmed publisher
	Baechler B, McKnight C, Pruchnicki P, Biro N, Reed B. Hindsight/RREB-1 functions in both the specification and differentiation of stem cells in the adult midgut of Drosophila. Biol Open. 2015;5:1-10 pubmed publisher
	Yan D, Neumüller R, BUCKNER M, Ayers K, Li H, Hu Y, et al. A regulatory network of Drosophila germline stem cell self-renewal. Dev Cell. 2014;28:459-73 pubmed publisher
	Zhou L, Luo H. Replication protein a links cell cycle progression and the onset of neurogenesis in Drosophila optic lobe development. J Neurosci. 2013;33:2873-88 pubmed publisher
	Xiao Q, Komori H, Lee C. klumpfuss distinguishes stem cells from progenitor cells during asymmetric neuroblast division. Development. 2012;139:2670-80 pubmed publisher
	Song Y, Lu B. Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila. Genes Dev. 2011;25:2644-58 pubmed publisher
	Wang W, Liu W, Wang Y, Zhou L, Tang X, Luo H. Notch signaling regulates neuroepithelial stem cell maintenance and neuroblast formation in Drosophila optic lobe development. Dev Biol. 2011;350:414-28 pubmed publisher
	Monastirioti M, Giagtzoglou N, Koumbanakis K, Zacharioudaki E, Deligiannaki M, Wech I, et al. Drosophila Hey is a target of Notch in asymmetric divisions during embryonic and larval neurogenesis. Development. 2010;137:191-201 pubmed publisher
	Ohayon D, Pattyn A, Venteo S, Valmier J, Carroll P, Garces A. Zfh1 promotes survival of a peripheral glia subtype by antagonizing a Jun N-terminal kinase-dependent apoptotic pathway. EMBO J. 2009;28:3228-43 pubmed publisher
	Doroquez D, Orr Weaver T, Rebay I. Split ends antagonizes the Notch and potentiates the EGFR signaling pathways during Drosophila eye development. Mech Dev. 2007;124:792-806 pubmed
	Lai E, Rubin G. Neuralized is essential for a subset of Notch pathway-dependent cell fate decisions during Drosophila eye development. Proc Natl Acad Sci U S A. 2001;98:5637-42 pubmed
	Demidenko Z, Badenhorst P, Jones T, Bi X, Mortin M. Regulated nuclear export of the homeodomain transcription factor Prospero. Development. 2001;128:1359-67 pubmed
	Gho M, Bellaiche Y, Schweisguth F. Revisiting the Drosophila microchaete lineage: a novel intrinsically asymmetric cell division generates a glial cell. Development. 1999;126:3573-84 pubmed
	Campbell G, Goring H, Lin T, Spana E, Andersson S, Doe C, et al. RK2, a glial-specific homeodomain protein required for embryonic nerve cord condensation and viability in Drosophila. Development. 1994;120:2957-66 pubmed

product information

Internal ID :

2150

Name :

Prospero (MR1A)

Depositor Name :

Doe, C.Q.

Depositor Institution :

University of Oregon

Date Deposited :

11/22/00

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Prospero protein

Antigen Species :

Drosophila

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila, yes. Grasshopper, mouse, zebrafish, no.

Initial Publication Pubmed ID :

7607085

Depositor Notes (Special Instructions) :

This antibody recognizes a transcription factor broadly expressed and conserved in vertebrate development.

Collections :

Drosophila antigens,Neurodevelopment,Transcription factors

Search Keywords :

Doe, Chris Q., Prospero protein , Drosophila , MIgG1, kappa light chain, Drosophila/Grasshopper/Mouse/Zebrafish, pros, , AB_528440, Monoclonal

Antigen Molecular Weight :

165 kDa predicted; 215 kDa observed

Gene :

pros

Uniprot ID :

P29617

Antibody Registry ID :

AB_528440

Immunogen :

Recombinant protein

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

23407946 21146517 20040486 17588724 11262236 10409503 22190460 26887495 29162808

Immunofluorescence Pubmed IDs :

24576427 22745313 17588724 11344304 26887495 27680705 29335529 29324767 27095493

Western Blot Pubmed IDs :

19745814

DSHB Growth Medium :

Iscove's

References (legacy) :

Development 120, 2957-2966.; Roux’s Arch. Dev. Biol. 204, 118-125.; Neuron 13, 269-287.; Mech. Dev. 53, 393-402.; Development 121, 3187-3195.; Development 121, 3989-3996.; Nature 376, 171-174.; Neuron 15, 1041-1051.; Neuron 14, 913-925.; Cell 82, 1025-1036.; Nature 377, 624-627.; Genes Dev. 9, 2384-2398.; Genes Dev. 9, 1223-1236.; Neuron 17, 27-41.; Dev. Biol. 180, 759-772.; Genes Dev. 10, 2167-2178.; Mech. Dev. 60, 95-107.; Curr. Biol. 6, 1146-1152.; Curr. Biol. 7, 827-835.; Proc. Natl. Acad. Sci. 94, 13005-13010.; Development 124, 673-681.; Development 124, 2307-2311.; Nature 390, 625-629.; Cell 90, 437-447.; Development 124, 4949-4958.; Development 124, 937-948.; Cell 90, 449-458.; Genes Dev. 11, 1396-1408.; Genes Dev. 12, 1858-1870.; Dev. Biol. 204, 478-487.; Nature 391, 792-795.; Development 125, 1845-1856.; Nature 393, 178-181.; Development 125, 4089-4098.; Genes Dev. 12, 3603-3612.; Genes Dev. 12, 1847-1857.; Genes Dev. 12, 1837-1846.; Development 125, 3301-3312.; Development 126, 2063-2071.; Development 126, 3573-3584.; Dev. Biol. 209, 381-398.; J. Cell Biol. 146, 1303-1318.; Development 126, 2083-2092.; Development 126, 4617-4622.; Proc. Natl. Acad. Sci. 96, 11382-11386.; J. Cell Sci. 112, 1541-1551.; Cell 103, 87-97.; Mech. Dev. 94, 3-12.; Proc. Natl. Acad. Sci. 97, 681(more. )

more info or order :

Developmental Studies Hybridoma Bank product webpage