puromycin | Developmental Studies Hybridoma Bank PMY-2A4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

puromycin

catalog :

PMY-2A4

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PMY-2A4

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
western blot; scFv; 1:30,000; fig st2	Milan G, Romanello V, Pescatore F, Armani A, Paik J, Frasson L, et al. Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy. Nat Commun. 2015;6:6670 pubmed publisher
	Xu Z, Lo W, Beck D, Schuch L, Oláhová M, Kopajtich R, et al. Bi-allelic Mutations in Phe-tRNA Synthetase Associated with a Multi-system Pulmonary Disease Support Non-translational Function. Am J Hum Genet. 2018;103:100-114 pubmed publisher
	Mazor K, Dong L, Mao Y, Swanda R, Qian S, Stipanuk M. Effects of single amino acid deficiency on mRNA translation are markedly different for methionine versus leucine. Sci Rep. 2018;8:8076 pubmed publisher
	Jacquemont S, Pacini L, Jønch A, Cencelli G, Rozenberg I, He Y, et al. Protein synthesis levels are increased in a subset of individuals with fragile X syndrome. Hum Mol Genet. 2018;27:2039-2051 pubmed publisher
	Sarikhani M, Mishra S, Maity S, Kotyada C, Wolfgeher D, Gupta M, et al. SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation. elife. 2018;7: pubmed publisher
	Shulla A, Randall G. Spatiotemporal analysis of hepatitis C virus infection. PLoS Pathog. 2015;11:e1004758 pubmed publisher
	Chambers J, Dalton L, Clarke H, Malzer E, Dominicus C, Patel V, et al. Actin dynamics tune the integrated stress response by regulating eukaryotic initiation factor 2α dephosphorylation. elife. 2015;4: pubmed publisher
	Desmet E, Anguish L, Parker J. Virus-mediated compartmentalization of the host translational machinery. MBio. 2014;5:e01463-14 pubmed publisher
	Park H, Park H, Ro S, Jang I, Semple I, Kim D, et al. Hepatoprotective role of Sestrin2 against chronic ER stress. Nat Commun. 2014;5:4233 pubmed publisher
	Voorhees R, Fernández I, Scheres S, Hegde R. Structure of the mammalian ribosome-Sec61 complex to 3.4 Å resolution. Cell. 2014;157:1632-43 pubmed publisher
	Al Jubran K, Wen J, Abdullahi A, Roy Chaudhury S, Li M, Ramanathan P, et al. Visualization of the joining of ribosomal subunits reveals the presence of 80S ribosomes in the nucleus. RNA. 2013;19:1669-83 pubmed publisher
	David A, Bennink J, Yewdell J. Emetine optimally facilitates nascent chain puromycylation and potentiates the ribopuromycylation method (RPM) applied to inert cells. Histochem Cell Biol. 2013;139:501-4 pubmed publisher
	David A, Dolan B, Hickman H, Knowlton J, Clavarino G, Pierre P, et al. Nuclear translation visualized by ribosome-bound nascent chain puromycylation. J Cell Biol. 2012;197:45-57 pubmed publisher

product information

Internal ID :

8316

Name :

PMY-2A4

Depositor Name :

Yewdell, Jonathan

Depositor Institution :

National Institutes of Health

Date Deposited :

3/9/12

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

puromycin

Antigen Species :

Streptomyces alboniger

Host Species :

mouse

Isotype :

MIgG2c

Isotype for catalog (legacy) :

IgG2a

Positive Tested Species Reactivity :

Rat

Initial Publication Pubmed ID :

22472439

Depositor Notes (Special Instructions) :

Fusion date: 07/21/2010. Fixation, 4% paraformaldehyde. The antigen puromycin inhibits translation by covalently associating with nascent chain within the ribosome. 2A4 allows puromycin detection in this condition. 2A4 also detects any puromycylated peptide. Medium: RPMI 1640 / Glutamine / Na pyruvate / 10% fetal bovine serum / Penicillin-streptomycin /gentamycin

Collections :

Microbe antibodies

Search Keywords :

Yewdell, Jonathan, puromycin, Streptomyces alboniger, MIgG2a, not applicable, , , , monoclonal, Microbe antibodies, Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/FACS

Antigen Molecular Weight :

0.5kDa

Antibody Registry ID :

AB_2619605

Synonyms (Alternate Clone Names) :

clone 4A12

Immunogen :

Puromycin (coupled to KLH) from Streptomyces alboniger bacterium. Chemical Composition: 3?-(?-Amino-p-methoxyhydrocinnamamido)-3?-deoxy-N,N-dimethyladenosine, 2HC1.

Clonality :

Monoclonal

Myeloma Strain :

SP2

Epitope Mapped :

Yes

Recommended Applications :

ELISA,FACS,Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

22472439 23229864 25227463 24129492 25822891 31416908

Western Blot Pubmed IDs :

25227463 26757818 24129492 24930395 24947615 25774599

Additional Information :

Puromycin is a structural analog of aminoacyl tRNAs that can be incorporated into nascent polypeptide chain and blocks elongation. This antibody is used to detect the subcellular localization of actively translating ribosomes by the ribopuromycylation method (RPM), and localizes puromycylated nascent chains on ribosomes immobilized by chain elongation inhibitors in living cells prior to processing for intracellular immunofluorescence and/or western blots. RRID:AB_2619605

DSHB Growth Medium :

Iscove's

References (legacy) :

J. Cell Biol. 197(1), 45-57.

more info or order :

Developmental Studies Hybridoma Bank product webpage