integrin alpha5beta1, Xenopus | Developmental Studies Hybridoma Bank P8D4 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha5beta1, Xenopus

catalog :

P8D4

clonality :

monoclonal

host :

house mouse

conjugate :

nonconjugated

clone name :

P8D4

reactivity :

Xenopus laevis

application :

immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Gouignard N, Maccarana M, Strate I, von Stedingk K, Malmstrom A, Pera E. Musculocontractural Ehlers-Danlos syndrome and neurocristopathies: dermatan sulfate is required for Xenopus neural crest cells to migrate and adhere to fibronectin. Dis Model Mech. 2016;9:607-20 pubmed publisher
Cousin H, DeSimone D, Alfandari D. PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis. Dev Biol. 2008;319:86-99 pubmed publisher
Davidson L, Marsden M, Keller R, DeSimone D. Integrin alpha5beta1 and fibronectin regulate polarized cell protrusions required for Xenopus convergence and extension. Curr Biol. 2006;16:833-44 pubmed
Cousin H, Alfandari D. A PTP-PEST-like protein affects alpha5beta1-integrin-dependent matrix assembly, cell adhesion, and migration in Xenopus gastrula. Dev Biol. 2004;265:416-32 pubmed
Alfandari D, Cousin H, Gaultier A, Hoffstrom B, DeSimone D. Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin. Dev Biol. 2003;260:449-64 pubmed
Davidson L, Hoffstrom B, Keller R, DeSimone D. Mesendoderm extension and mantle closure in Xenopus laevis gastrulation: combined roles for integrin alpha(5)beta(1), fibronectin, and tissue geometry. Dev Biol. 2002;242:109-29 pubmed

product information

Internal ID :

23794

Name :

P8D4

Depositor Name :

DeSimone, D.W.

Depositor Institution :

University of Virginia School of Medicine

Depositor Website :

https://med.virginia.edu/cell-biology/lab-snap-shot/desimone-lab/

Date Deposited :

6/23/15

Allow Hybridoma Distribution :

Cells Available (legacy) :

Antigen :

integrin alpha5beta1, Xenopus

Antigen Species :

Xenopus laevis

Host Species :

mouse

Isotype :

MIgG1

Positive Tested Species Reactivity :

Xenopus laevis

Initial Publication Pubmed ID :

11820810

Depositor Notes (Special Instructions) :

This antibody blocks binding of alpha5 beta 1 integrin to the synergy site in type III-9 fibrobnectin repeat.

Collections :

Cell adhesion,Xenopus

Search Keywords :

DeSimone, D.W., integrin alpha5beta1, Xenopus laevis, MIgG1, Xenopus laevis, itga5, VLA-5, , Monoclonal, Xenopus/Cell adhesion, Function blocking/immunoprecipitation/Immunofluorescence

Antigen Molecular Weight :

135 kDa reduced, 155kDa unreduced

Gene :

itga5

Alternate Gene Name(s) :

VLA-5

Uniprot ID :

Q06274

Antibody Registry ID :

AB_2722121

Immunogen :

S3-1 cells (derived from St. 18 dorsal explant)

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SpZ/O-Ag 14

Epitope Mapped :

Epitope Location or Sequence :

recognizes a composite epitope on alpha5/beta1 native heterodimer integrin.

Recommended Applications :

Function Blocking,Immunofluorescence,Immunoprecipitation

Immunohistochemistry Pubmed IDs :

11820810

Immunofluorescence Pubmed IDs :

12921745

Immunoprecipitation Pubmed IDs :

12921745 11820810 18495106 27101845

Function Blocking Pubmed IDs :

11820810 16682346 12921745 14732402

Additional Information :

RRID:AB_2722121

DSHB Growth Medium :

Iscove's

more info or order :

Developmental Studies Hybridoma Bank product webpage