product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Disabled protein
catalog :
P6E11
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P6E11
reactivity :
fruit fly
application :
western blot, ELISA, immunohistochemistry
citations: 4
Reference
Kannan R, Cox E, Wang L, Kuzina I, Gu Q, Giniger E. Tyrosine phosphorylation and proteolytic cleavage of Notch are required for non-canonical Notch/Abl signaling in Drosophila axon guidance. Development. 2018;145: pubmed publisher
Kannan R, Song J, Karpova T, Clarke A, Shivalkar M, Wang B, et al. The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila. Development. 2017;144:487-498 pubmed publisher
Song J, Kannan R, Merdes G, Singh J, Mlodzik M, Giniger E. Disabled is a bona fide component of the Abl signaling network. Development. 2010;137:3719-27 pubmed publisher
Le Gall M, De Mattei C, Giniger E. Molecular separation of two signaling pathways for the receptor, Notch. Dev Biol. 2008;313:556-67 pubmed
product information
Internal ID :
3279
Name :
P6E11
Depositor Name :
Giniger, E.
Depositor Institution :
NIH (NINDS)
Date Deposited :
3/12/04
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Disabled protein
Antigen Species :
Drosophila
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Drosophila
Species Tested (legacy) :
Drosophila
Initial Publication Pubmed ID :
18062953
Depositor Notes (Special Instructions) :
P4D11 is recommended for Immunoprecipitation, and P6E11 is recommended for Immunohistochemistry and Immunoblotting.
Collections :
Cell signaling,Drosophila antigens
Search Keywords :
Giniger, E., Disabled protein, Drosophila, MIgG1, Drosophila, Dab, CG9695, AB_528201, monoclonal, Cell signaling/Drosophila antigens, Immunohistochemistry/Western Blot/ELISA
Antigen Molecular Weight :
Predicted: 227 and 243 kDa (at least two isoforms); Apparent: around 280 kDa
Gene :
DAB
Alternate Gene Name(s) :
CG9695
Uniprot ID :
P98081
Antibody Registry ID :
AB_528201
Immunogen :
Recombinant Drosophila Disabled protein (N-terminal 2/3)/His(6)-tagged protein
Immunogen Sequence :
Partial protein
Clonality :
Monoclonal
Epitope Mapped :
Yes
Epitope Location or Sequence :
N-terminal 2/3 of the protein
Epitope Map PubMed ID :
18062953
Recommended Applications :
ELISA,Immunohistochemistry,Western Blot
Immunoblotting (legacy) :
Good
Immunohistochemistry Pubmed IDs :
18062953
Western Blot Pubmed IDs :
18062953 20940230
ELISA Pubmed IDs :
18062953
Additional Information :
P6E11 gives some nonspecific background by Immunohistochemistry but is better than P4D11 [PMID 18062953]. PE611 has been reported to recognize both isoforms reported by Gertler et al (1993; [PMID 7680635]) [PMID 20940230].
DSHB Growth Medium :
RPMI
References (legacy) :
Dev. Biol. 313, 556-567.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US