integrin beta-1 (CD29), human | Developmental Studies Hybridoma Bank P5D2 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin beta-1 (CD29), human

catalog :

P5D2

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P5D2

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, blocking or activating experiments, western blot knockout validation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 28

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:17; loading ...; fig 2c	Huet Calderwood C, Rivera Molina F, Iwamoto D, Kromann E, Toomre D, Calderwood D. Novel ecto-tagged integrins reveal their trafficking in live cells. Nat Commun. 2017;8:570 pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig 4h	Sahgal P, Alanko J, Icha J, Paatero I, Hamidi H, Arjonen A, et al. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. J Cell Sci. 2019;132: pubmed publisher
immunocytochemistry; human; 1:100; fig s4	Qi L, Jafari N, Li X, Chen Z, Li L, Hytönen V, et al. Talin2-mediated traction force drives matrix degradation and cell invasion. J Cell Sci. 2016;129:3661-3674 pubmed
flow cytometry; human	Iwai L, Payne L, Luczynski M, Chang F, Xu H, Clinton R, et al. Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants. Biochem J. 2013;454:501-13 pubmed publisher
blocking or activating experiments; human; 10 ug/ml immunocytochemistry; human; 10 ug/ml; fig s9f	Xu H, Bihan D, Chang F, Huang P, Farndale R, Leitinger B. Discoidin domain receptors promote Î±1Î²1- and Î±2Î²1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PLoS ONE. 2012;7:e52209 pubmed publisher
	Bekier M, Wang L, Li J, Huang H, Tang D, Zhang X, et al. Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function. Mol Biol Cell. 2017;28:2833-2842 pubmed publisher
	Hou S, Isaji T, Hang Q, Im S, Fukuda T, Gu J. Distinct effects of Î²1 integrin on cell proliferation and cellular signaling in MDA-MB-231 breast cancer cells. Sci Rep. 2016;6:18430 pubmed publisher
	Isaji T, Im S, Gu W, Wang Y, Hang Q, Lu J, et al. An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J Biol Chem. 2014;289:20694-705 pubmed
	Flannagan R, Canton J, Furuya W, Glogauer M, Grinstein S. The phosphatidylserine receptor TIM4 utilizes integrins as coreceptors to effect phagocytosis. Mol Biol Cell. 2014;25:1511-22 pubmed publisher
	Hämälistö S, Pouwels J, De Franceschi N, Saari M, Ivarsson Y, Zimmermann P, et al. A ZO-1/?5?1-integrin complex regulates cytokinesis downstream of PKC? in NCI-H460 cells plated on fibronectin. PLoS ONE. 2013;8:e70696 pubmed publisher
	Xiang Y, Zhang X, Nix D, Katoh T, Aoki K, Tiemeyer M, et al. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nat Commun. 2013;4:1659 pubmed publisher
	Lee J, Ballikaya S, Schonig K, Ball C, Glimm H, Kopitz J, et al. Transforming growth factor beta receptor 2 (TGFBR2) changes sialylation in the microsatellite unstable (MSI) Colorectal cancer cell line HCT116. PLoS ONE. 2013;8:e57074 pubmed publisher
	Taubenberger A, Quent V, Thibaudeau L, Clements J, Hutmacher D. Delineating breast cancer cell interactions with engineered bone microenvironments. J Bone Miner Res. 2013;28:1399-411 pubmed publisher
	Gautrot J, Wang C, Liu X, Goldie S, Trappmann B, Huck W, et al. Mimicking normal tissue architecture and perturbation in cancer with engineered micro-epidermis. Biomaterials. 2012;33:5221-9 pubmed publisher
	Mai A, Veltel S, Pellinen T, Padzik A, Coffey E, Marjomaki V, et al. Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration. J Cell Biol. 2011;194:291-306 pubmed publisher
	Xia B, Joubert A, Groves B, Vo K, Ashraf D, Djavaherian D, et al. Modulation of cell adhesion and migration by the histone methyltransferase subunit mDpy-30 and its interacting proteins. PLoS ONE. 2010;5:e11771 pubmed publisher
	Rowland T, Miller L, Blaschke A, Doss E, Bonham A, Hikita S, et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 2010;19:1231-40 pubmed publisher
	Vascotto S, Griffith M. Localization of candidate stem and progenitor cell markers within the human cornea, limbus, and bulbar conjunctiva in vivo and in cell culture. Anat Rec A Discov Mol Cell Evol Biol. 2006;288:921-31 pubmed
	Muenzner P, Rohde M, Kneitz S, Hauck C. CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells. J Cell Biol. 2005;170:825-36 pubmed
	Levesque J, Takada Y, Puzon McLaughlin W, Simmons P. A novel monoclonal antibody recognizing a cation-dependent epitope within the regulatory loop of human beta(1) integrin (CD29). Hybrid Hybridomics. 2002;21:253-60 pubmed
	Setty S, Kim Y, Fields G, Clegg D, Wayner E, Tsilibary E. Interactions of type IV collagen and its domains with human mesangial cells. J Biol Chem. 1998;273:12244-9 pubmed
	Skubitz A, Bast R, Wayner E, Letourneau P, Wilke M. Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. Am J Pathol. 1996;148:1445-61 pubmed
	Skubitz A, Grossman M, McCarthy J, Wayner E, Cameron J. The decreased adhesion of Y79 retinoblastoma cells to extracellular matrix proteins is due to a deficit of integrin receptors. Invest Ophthalmol Vis Sci. 1994;35:2820-33 pubmed
	Dittel B, McCarthy J, Wayner E, LeBien T. Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1). Blood. 1993;81:2272-82 pubmed
	Wyrick P, Davis C, Wayner E. Chlamydia trachomatis does not bind to alpha beta 1 integrins to colonize a human endometrial epithelial cell line cultured in vitro. Microb Pathog. 1994;17:159-66 pubmed
	Seltzer J, Lee A, Akers K, Sudbeck B, Southon E, Wayner E, et al. Activation of 72-kDa type IV collagenase/gelatinase by normal fibroblasts in collagen lattices is mediated by integrin receptors but is not related to lattice contraction. Exp Cell Res. 1994;213:365-74 pubmed
	Bhatia R, Wayner E, McGlave P, Verfaillie C. Interferon-alpha restores normal adhesion of chronic myelogenous leukemia hematopoietic progenitors to bone marrow stroma by correcting impaired beta 1 integrin receptor function. J Clin Invest. 1994;94:384-91 pubmed
	Joneckis C, Ackley R, Orringer E, Wayner E, Parise L. Integrin alpha 4 beta 1 and glycoprotein IV (CD36) are expressed on circulating reticulocytes in sickle cell anemia. Blood. 1993;82:3548-55 pubmed

product information

Internal ID :

1057

Name :

P5D2

Depositor Name :

Wayner, E.A.

Depositor Institution :

Fred Hutchison Cancer Research Center

Date Deposited :

8/4/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin beta-1 (CD29), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

7683214

Depositor Notes (Special Instructions) :

Inhibits binding of beta1 integrins. This antibody doesn't appear to work for western blotting.

Collections :

CD antigens,Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Wayner, E.A., integrin beta-1 (CD29), human, Human, MIgG1, Human, ITGB1, CD29, GPIIA, FNRB, MDF2, MSK12, VLA-4beta, AB_528308, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling/Extracellular matrix proteins/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function Blocking/FACS

Antigen Molecular Weight :

Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa

Gene :

ITGB1

Alternate Gene Name(s) :

CD29, GPIIA, FNRB, MDF2, MSK12, VLA-4beta

Uniprot ID :

P05556

Antibody Registry ID :

AB_528308

Synonyms (Alternate Clone Names) :

CD29

Immunogen :

Human epidermal keratinocytes

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Fox NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

NKGEVFNELVGK (a.a. 207-218)

Epitope Map PubMed ID :

12193278

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

8623916

Immunofluorescence Pubmed IDs :

16779811 23967087

Immunoprecipitation Pubmed IDs :

7505118 9575174 23552074 23468914

Function Blocking Pubmed IDs :

7535373 7683214 7519563 9575174 7518835 8188477 16115956 23362043 24895123

FACS Pubmed IDs :

7505118 8623916 7518835 8188477 16115956 24895123

Pubmed IDs :

19811096

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 121, 1141-1152.; PLos ONE 8(2): e57074. doi:10.1371/journal.pone.0057074.

more info or order :

Developmental Studies Hybridoma Bank product webpage