integrin alpha-4 (CD49d) | Developmental Studies Hybridoma Bank P4G9 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha-4 (CD49d)

catalog :

P4G9

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P4G9

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 6

Reference
Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
Kapila Y, Wang S, Johnson P. Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases. J Biol Chem. 1999;274:30906-13 pubmed
Xia M, Sreedharan S, Dazin P, Damsky C, Goetzl E. Integrin-dependent role of human T cell matrix metalloproteinase activity in chemotaxis through a model basement membrane. J Cell Biochem. 1996;61:452-8 pubmed
Schiffer S, Hemler M, Lobb R, Tizard R, Osborn L. Molecular mapping of functional antibody binding sites of alpha 4 integrin. J Biol Chem. 1995;270:14270-3 pubmed
Miettinen M, Castello R, Wayner E, Schwarting R. Distribution of VLA integrins in solid tumors. Emergence of tumor-type-related expression. Patterns in carcinomas and sarcomas. Am J Pathol. 1993;142:1009-18 pubmed
Wayner E, Garcia Pardo A, Humphries M, McDonald J, Carter W. Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J Cell Biol. 1989;109:1321-30 pubmed

product information

Internal ID :

7734

Name :

P4G9

Depositor Name :

Wayner, E.A. / Carter, W.G.

Depositor Institution :

Fred Hutchison Cancer Research Center,

Date Deposited :

10/11/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin alpha-4 (CD49d)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG2b

Isotype for catalog (legacy) :

IgG2b

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

2527858

Depositor Notes (Special Instructions) :

P4G9 stimulates aggregation of T cells. IHC is validated using cryostat frozen sections.

Collections :

CD antigens,Cell adhesion,Human

Search Keywords :

Wayner, E.A. / Carter, W.G. , integrin alpha-4 (CD49d) , Human, MIgG2b, Human, ITGA4, CD49D, AB_2619601, Monoclonal, Human/Cell adhesion/Cluster determinant antigens, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function blocking/Flow cytometry

Antigen Molecular Weight :

114.9 kDa

Gene :

ITGA4

Alternate Gene Name(s) :

CD49D

Uniprot ID :

P13612

Antibody Registry ID :

AB_2619601

Synonyms (Alternate Clone Names) :

CD49d

Immunogen :

HT1080 human fibrosarcoma cell line

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminus 1-100

Epitope Map PubMed ID :

7782282

Recommended Applications :

FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

7682756

Immunofluorescence Pubmed IDs :

2527858

Immunoprecipitation Pubmed IDs :

2527858 7782282

Function Blocking Pubmed IDs :

2527858 10521484 8761949

FACS Pubmed IDs :

7782282

Additional Information :

RRID:AB_2619601

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Bio. 109, 1321-1330.; Am. J. Path. 142, 1009-1018.; Exp. Hematol. 24, 158-168.; J. Cell. Biochem. 61, 452-458.; J. Biol. Chem. 274(43), 30906-30913.; Methods in Enzymology 426, 117-153.

more info or order :

Developmental Studies Hybridoma Bank product webpage