integrin beta-1 (CD29), human | Developmental Studies Hybridoma Bank P4G11 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin beta-1 (CD29), human

catalog :

P4G11

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P4G11

reactivity :

human

application :

immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 11

Reference
Starchenko A, Graves Deal R, Yang Y, Li C, Zent R, Singh B, et al. Clustering of integrin Î±5 at the lateral membrane restores epithelial polarity in invasive colorectal cancer cells. Mol Biol Cell. 2017;28:1288-1300 pubmed publisher
Taubenberger A, Bray L, Haller B, Shaposhnykov A, Binner M, Freudenberg U, et al. 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater. 2016;36:73-85 pubmed publisher
Williams K, Coppolino M. SNARE-dependent interaction of Src, EGFR and ?1 integrin regulates invadopodia formation and tumor cell invasion. J Cell Sci. 2014;127:1712-25 pubmed publisher
Katoh D, Nagaharu K, Shimojo N, Hanamura N, Yamashita M, Kozuka Y, et al. Binding of ?v?1 and ?v?6 integrins to tenascin-C induces epithelial-mesenchymal transition-like change of breast cancer cells. Oncogenesis. 2013;2:e65 pubmed publisher
Etemad B, Gonzalez O, McDonough S, Pena Cruz V, Sagar M. Early infection HIV-1 envelope V1-V2 genotypes do not enhance binding or replication in cells expressing high levels of ?4?7 integrin. J Acquir Immune Defic Syndr. 2013;64:249-53 pubmed publisher
Mai A, Veltel S, Pellinen T, Padzik A, Coffey E, Marjomaki V, et al. Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration. J Cell Biol. 2011;194:291-306 pubmed publisher
Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
Dorfleutner A, Hintermann E, Tarui T, Takada Y, Ruf W. Cross-talk of integrin alpha3beta1 and tissue factor in cell migration. Mol Biol Cell. 2004;15:4416-25 pubmed
Wayner E, Gil S, Murphy G, Wilke M, Carter W. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes. J Cell Biol. 1993;121:1141-52 pubmed
Dittel B, McCarthy J, Wayner E, LeBien T. Regulation of human B-cell precursor adhesion to bone marrow stromal cells by cytokines that exert opposing effects on the expression of vascular cell adhesion molecule-1 (VCAM-1). Blood. 1993;81:2272-82 pubmed
Wyrick P, Davis C, Wayner E. Chlamydia trachomatis does not bind to alpha beta 1 integrins to colonize a human endometrial epithelial cell line cultured in vitro. Microb Pathog. 1994;17:159-66 pubmed

product information

Internal ID :

1052

Name :

P4G11

Depositor Name :

Wayner, E.A.

Depositor Institution :

Fred Hutchison Cancer Research Center

Date Deposited :

8/4/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin beta-1 (CD29), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

8501119

Depositor Notes (Special Instructions) :

Stimulates binding of ?1 integrins. This antibody doesn't appear to work for western blotting.

Collections :

CD antigens,Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Wayner, E.A., integrin beta-1 (CD29), human, Human, MIgG1, Human, ITGB1, CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA, AB_528307, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling/Extracellular matrix proteins/Neurodevelopment, Immunofluorescence/Immunoprecipitation/Function Blocking/FACS

Antigen Molecular Weight :

Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa

Gene :

ITGB1

Alternate Gene Name(s) :

CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA

Uniprot ID :

P05556

Antibody Registry ID :

AB_528307

Synonyms (Alternate Clone Names) :

CD29

Immunogen :

Human monocytic U937 cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

Fox NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

extracellular

Epitope Map PubMed ID :

8501119

Recommended Applications :

FACS,Immunofluorescence,Immunoprecipitation

Immunoblotting (legacy) :

Immunofluorescence Pubmed IDs :

7535373 23958855

Immunoprecipitation Pubmed IDs :

8501119

Function Blocking Pubmed IDs :

8501119 24496451 7535373 7683214

FACS Pubmed IDs :

26971667 7535373

Pubmed IDs :

15254262 23797693 17697883

Additional Information :

P4G11 is not a function blocking mAb. It's functional effect is enhancing. P4G11 recognizes a Ca2+-dependent epitope.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 121, 1141-1152.

more info or order :

Developmental Studies Hybridoma Bank product webpage