Wash | Developmental Studies Hybridoma Bank P4C9-Wash product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Wash

catalog :

P4C9-Wash

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P4C9-Wash

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 1

Reference
Rodriguez Mesa E, Abreu Blanco M, Rosales Nieves A, Parkhurst S. Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins. Dev Dyn. 2012;241:608-26 pubmed publisher

product information

Internal ID :

8086

Name :

P4C9-Wash

Depositor Name :

Parkhurst, S.

Depositor Institution :

Fred Hutchinson Cancer Research Center

Date Deposited :

10/10/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Wash

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila melanogaster

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

22275148

Depositor Notes (Special Instructions) :

Fixation for immunostaining: 4% paraformaldehyde in PBS or standard embryo protocol.

Collections :

Cell signaling,Cytoskeleton,Drosophila antigens

Search Keywords :

Parkhurst, S., Wash, Drosophila melanogaster, MIgG1, Drosophila melanogaster, wash, CG13176, AB_2618391, monoclonal, Cell signaling/Drosophila antigens/Cytoskeletal elements, Immunofluorescence/Western blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 53 kDa; Apparent: 75 kDa

Gene :

wash

Alternate Gene Name(s) :

CG13176

Uniprot ID :

Q7JW27

Antibody Registry ID :

AB_2618391

Immunogen :

Recombinant Wash/GST fusion protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Western Blot Pubmed IDs :

22275148

Additional Information :

wash: Wiskott-Aldrich syndrome protein. Wash polymerizes branched actin through activation of the Arp2/3 complex, as well as bundles and/or crosslinks actin and microtubules. P4C9 gives additional bands and doesn't appear to work as well as P3H3 (a different anti-Wash clone in the DSHB collection) for western blotting.

DSHB Growth Medium :

RPMI

References (legacy) :

Dev. Dyn. 241, 608-626.

more info or order :

Developmental Studies Hybridoma Bank product webpage