integrin beta-1 (CD29), human | Developmental Studies Hybridoma Bank P4C10 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin beta-1 (CD29), human

catalog :

P4C10

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P4C10

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 2a western blot; human; loading ...; fig 2b	Brasher M, Martynowicz D, Grafinger O, Hucik A, Shanks Skinner E, Uniacke J, et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem. 2017;292:16199-16210 pubmed publisher
immunoprecipitation; human immunocytochemistry; human	Williams K, McNeilly R, Coppolino M. SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion. Mol Biol Cell. 2014;25:2061-70 pubmed publisher
	Petridou N, Skourides P. A ligand-independent integrin Î²1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed publisher
	Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
	Dorfleutner A, Hintermann E, Tarui T, Takada Y, Ruf W. Cross-talk of integrin alpha3beta1 and tissue factor in cell migration. Mol Biol Cell. 2004;15:4416-25 pubmed
	Gronthos S, Stewart K, Graves S, Hay S, Simmons P. Integrin expression and function on human osteoblast-like cells. J Bone Miner Res. 1997;12:1189-97 pubmed
	Wayner E, Gil S, Murphy G, Wilke M, Carter W. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes. J Cell Biol. 1993;121:1141-52 pubmed
	Takada Y, Puzon W. Identification of a regulatory region of integrin beta 1 subunit using activating and inhibiting antibodies. J Biol Chem. 1993;268:17597-601 pubmed
	Wayner E, Carter W. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987;105:1873-84 pubmed
	Carter W, Kaur P, Gil S, Gahr P, Wayner E. Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J Cell Biol. 1990;111:3141-54 pubmed
	Garcia Pardo A, Wayner E, Carter W, Ferreira O. Human B lymphocytes define an alternative mechanism of adhesion to fibronectin. The interaction of the alpha 4 beta 1 integrin with the LHGPEILDVPST sequence of the type III connecting segment is sufficient to promote cell attachment. J Immunol. 1990;144:3361-6 pubmed
	Wayner E, Orlando R, Cheresh D. Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol. 1991;113:919-29 pubmed
	Schwartz B, Wayner E, Carlos T, Ochs H, Harlan J. Identification of surface proteins mediating adherence of CD11/CD18-deficient lymphoblastoid cells to cultured human endothelium. J Clin Invest. 1990;85:2019-22 pubmed
	Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed
	Iida J, Skubitz A, Furcht L, Wayner E, McCarthy J. Coordinate role for cell surface chondroitin sulfate proteoglycan and alpha 4 beta 1 integrin in mediating melanoma cell adhesion to fibronectin. J Cell Biol. 1992;118:431-44 pubmed
	Wayner E, Kovach N. Activation-dependent recognition by hematopoietic cells of the LDV sequence in the V region of fibronectin. J Cell Biol. 1992;116:489-97 pubmed
	Leavesley D, Ferguson G, Wayner E, Cheresh D. Requirement of the integrin beta 3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen. J Cell Biol. 1992;117:1101-7 pubmed

product information

Internal ID :

7750

Name :

P4C10

Depositor Name :

Wayner, E.A. / Carter, W.G.

Depositor Institution :

Fred Hutchison Cancer Research Center

Date Deposited :

10/11/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin beta-1 (CD29), human

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

2822727

Depositor Notes (Special Instructions) :

P4C10 inhibits endodermal cell and keratinocyte attachment to collagen type 1, fibronectin and laminin; also inhibits cell-cell adhesion. Fixation for immunostaining: formalin, 4% paraformaldehyde, acetone-methanol, ice-cold methanol. The epitope recognized by P4C10 is not trypsin sensitive. This antibody doesn't appear to work for western blotting as it recognizes the native protein.

Collections :

CD antigens,Cell adhesion,Cell signaling,Extracellular matrix proteins,Human,Neurodevelopment

Search Keywords :

Wayner, E.A. / Carter, W.G., integrin beta-1 (CD29), human, Human, MIgG1, Human, ITGB1, CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA, AB_2619600, monoclonal, Cell adhesion/Cluster Determinant antigens/Human/Cell signaling/Extracellular matrix proteins/Neurodevelopment, Immunohistochemistry/FFPE/Immunofluorescence/Western Blot/Immunoprecipitation/ELISA/Function Blocking/FACS

Antigen Molecular Weight :

Multiple isoforms; Predicted: 87 to 92 kDa; Apparent: 120 kDa

Gene :

ITGB1

Alternate Gene Name(s) :

CD29, FNRB, MDF2, MSK12, VLA-4beta, GPIIA

Uniprot ID :

P05556

Antibody Registry ID :

AB_2619600

Synonyms (Alternate Clone Names) :

CD29

Immunogen :

Human fibrosarcoma HT-1080 cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

NKGEVFNELVGK (a.a. 207-218)

Epitope Map PubMed ID :

7688727

Recommended Applications :

ELISA,FACS,FFPE,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

2269668 8501119

Immunofluorescence Pubmed IDs :

2269668

Western Blot Pubmed IDs :

24807903 28798239 28798239

Immunoprecipitation Pubmed IDs :

2822727 8501119

ELISA Pubmed IDs :

1693380

Function Blocking Pubmed IDs :

2822727 1691191 2269668 26952307 1374415 1693380 1709170 1530947 8501119 1629241 2139453 9258748

FACS Pubmed IDs :

1693380 1629241

Pubmed IDs :

17697883 15254262

Additional Information :

While this antibody has been reported not to work for western blotting by the hybridoma contributor (Wayner, E.A.), Williams et al. have reported using it succesfully [PMID: 24807903]. This antibody will recognize all known isoforms.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell. Biol. 105, 1873-1884.; J. Cell Biol. 110, 1387-1404.; J. Cell Biol. 111, 3141-3154.; Methods in Enzymology 426, 117-153.

more info or order :

Developmental Studies Hybridoma Bank product webpage