Wash | Developmental Studies Hybridoma Bank P3H3-Wash product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Wash

catalog :

P3H3-Wash

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P3H3-Wash

reactivity :

fruit fly

application :

western blot, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; scFv; fig 4	Kwon S, Grisan V, Jang B, Herbert J, Badenhorst P. Genome-Wide Mapping Targets of the Metazoan Chromatin Remodeling Factor NURF Reveals Nucleosome Remodeling at Enhancers, Core Promoters and Gene Insulators. PLoS Genet. 2016;12:e1005969 pubmed publisher
	Tran D, Masedunskas A, Weigert R, Ten Hagen K. Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo. Nat Commun. 2015;6:10098 pubmed publisher
	Wang S, Tan K, Agosto M, Xiong B, Yamamoto S, Sandoval H, et al. The retromer complex is required for rhodopsin recycling and its loss leads to photoreceptor degeneration. PLoS Biol. 2014;12:e1001847 pubmed publisher
	Rodriguez Mesa E, Abreu Blanco M, Rosales Nieves A, Parkhurst S. Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins. Dev Dyn. 2012;241:608-26 pubmed publisher
	Liu R, Abreu Blanco M, Barry K, Linardopoulou E, Osborn G, Parkhurst S. Wash functions downstream of Rho and links linear and branched actin nucleation factors. Development. 2009;136:2849-60 pubmed publisher

product information

Internal ID :

6203

Name :

P3H3-Wash

Depositor Name :

Parkhurst, S.

Depositor Institution :

Fred Hutchinson Cancer Research Center

Date Deposited :

8/17/09

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Wash

Antigen Species :

Drosophila melanogaster

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Drosophila

Species Tested (legacy) :

Drosophila

Initial Publication Pubmed ID :

19633175

Collections :

Cell signaling,Cytoskeleton,Drosophila antigens

Search Keywords :

Parkhurst, S., Wash, Drosophila melanogaster, MIgG1, Drosophila, wash, CG13176, AB_2618389, monoclonal, Cell signaling/Drosophila antigens/Cytoskeletal elements, Immunofluorescence/Western blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: 53 kDa; Apparent: 75 kDa

Gene :

wash

Alternate Gene Name(s) :

CG13176

Uniprot ID :

Q7JW27

Antibody Registry ID :

AB_2618389

Immunogen :

Recombinant Wash/GST fusion protein

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Epitope Mapped :

Recommended Applications :

Immunofluorescence,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

19633175 26639106 22275148 24781186

Western Blot Pubmed IDs :

19633175 27046080 22275148

Immunoprecipitation Pubmed IDs :

19633175 27046080

Additional Information :

wash: Wiskott-Aldrich syndrome protein. Wash polymerizes branched actin through activation of the Arp2/3 complex, as well as bundles and/or crosslinks actin and microtubules.

DSHB Growth Medium :

RPMI

References (legacy) :

Development 136, 2849-2860.

more info or order :

Developmental Studies Hybridoma Bank product webpage