product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin alpha V subunit (CD51, fibronectin receptor alpha subunit)
catalog :
P3G8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P3G8
reactivity :
human
application :
ELISA, immunohistochemistry, immunoprecipitation, FACS
more info or order :
citations: 11
Reference |
---|
Zhang Y, Lu H, Dazin P, Kapila Y. Functional differences between integrin alpha4 and integrins alpha5/alphav in modulating the motility of human oral squamous carcinoma cells in response to the V region and heparin-binding domain of fibronectin. Exp Cell Res. 2004;295:48-58 pubmed
|
Ellis P, Metcalfe J, Hyvonen M, Kemp P. Adhesion of endothelial cells to NOV is mediated by the integrins alphavbeta3 and alpha5beta1. J Vasc Res. 2003;40:234-43 pubmed
|
Kim J, Jeong H, Nam J, Lee B, Choi J, Park R, et al. Identification of motifs in the fasciclin domains of the transforming growth factor-beta-induced matrix protein betaig-h3 that interact with the alphavbeta5 integrin. J Biol Chem. 2002;277:46159-65 pubmed
|
Vihinen P, Nikkola J, Vlaykova T, Hahka Kemppinen M, Talve L, Heino J, et al. Prognostic value of beta1 integrin expression in metastatic melanoma. Melanoma Res. 2000;10:243-51 pubmed
|
Skubitz A, Grossman M, McCarthy J, Wayner E, Cameron J. The decreased adhesion of Y79 retinoblastoma cells to extracellular matrix proteins is due to a deficit of integrin receptors. Invest Ophthalmol Vis Sci. 1994;35:2820-33 pubmed
|
Weinacker A, Chen A, Agrez M, Cone R, Nishimura S, Wayner E, et al. Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor. J Biol Chem. 1994;269:6940-8 pubmed
|
Maldonado B, Furcht L. Involvement of integrins with adhesion-promoting, heparin-binding peptides of type IV collagen in cultured human corneal epithelial cells. Invest Ophthalmol Vis Sci. 1995;36:364-72 pubmed
|
Levesque J, Leavesley D, Niutta S, Vadas M, Simmons P. Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins. J Exp Med. 1995;181:1805-15 pubmed
|
Wayner E, Orlando R, Cheresh D. Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol. 1991;113:919-29 pubmed
|
product information
Internal ID :
7926
Name :
P3G8
Depositor Name :
Wayner, E.A.
Depositor Institution :
Fred Hutchison Cancer Research Center
Date Deposited :
10/11/11
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
integrin alpha V subunit (CD51, fibronectin receptor alpha subunit)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human
Initial Publication Pubmed ID :
1709170
Depositor Notes (Special Instructions) :
Immunostaining: Fixation, frozen (acetone), paraformaldehyde; Paraffin, yes – formalin fixed; Special protocols, Antigen Retrieval with 10mM sodium citrate pH 6.0. Doesn't appear to work for Immunoblotting.
Collections :
CD antigens,Cell signaling,Human
Search Keywords :
Wayner, E.A., integrin alpha V subunit (CD51, fibronectin receptor alpha subunit), Human, MIgG1, Human, ITGAV, MSK8, VNRA, CD51, VTNR, AB_2619589, monoclonal, Human/Cell signaling/Cluster Determinant antigens, Immunohistochemistry/FFPE/Immunoprecipitation/ELISA/FACS
Antigen Molecular Weight :
Predicted: 111 to 116 kDa
Gene :
ITGAV
Alternate Gene Name(s) :
MSK8, VNRA, CD51, VTNR
Uniprot ID :
P06756
Antibody Registry ID :
AB_2619589
Synonyms (Alternate Clone Names) :
CD51
Immunogen :
Small lung carcinoma cells (UCLA P3).
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1/FOX-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
The epitope is located in the extracellular region; aa 528 of human alpha-v integrin (aa 558 of Uniprot P06756-1) is required for P3G8 binding.
Epitope Map PubMed ID :
23840404
Recommended Applications :
ELISA,FACS,FFPE,Immunohistochemistry,Immunoprecipitation
Immunoblotting (legacy) :
no
Immunohistochemistry Pubmed IDs :
10890378
Immunoprecipitation Pubmed IDs :
8120056
FACS Pubmed IDs :
8188477 15051489 7536795 23840404 12270930 7843907
Pubmed IDs :
1709170 12902636 18664572
Additional Information :
P3G8 recognizes all isoforms.
DSHB Growth Medium :
RPMI
References (legacy) :
J. Biol. Chem. 269(9), 6940-6948.; J. Vasc. Res. 40, 234-243.; Exp. Cell Res. 295(1), 45-58.; J. Biol. Chem. 283, 27612-27619.
more info or order :
company information
Developmental Studies Hybridoma Bank
review
product type
related products
browse more products
questions and comments