integrin alpha 2 (CD49b) | Developmental Studies Hybridoma Bank P1H6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha 2 (CD49b)

catalog :

P1H6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1H6

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 8

Reference
Carragher N, Levkau B, Ross R, Raines E. Degraded collagen fragments promote rapid disassembly of smooth muscle focal adhesions that correlates with cleavage of pp125(FAK), paxillin, and talin. J Cell Biol. 1999;147:619-30 pubmed
Koyama H, Raines E, Bornfeldt K, Roberts J, Ross R. Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors. Cell. 1996;87:1069-78 pubmed
Skubitz A, Bast R, Wayner E, Letourneau P, Wilke M. Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. Am J Pathol. 1996;148:1445-61 pubmed
Symington B, Takada Y, Carter W. Interaction of integrins alpha 3 beta 1 and alpha 2 beta 1: potential role in keratinocyte intercellular adhesion. J Cell Biol. 1993;120:523-35 pubmed
Flickinger K, Judware R, Lechner R, Carter W, Culp L. Integrin expression in human neuroblastoma cells with or without N-myc amplification and in ectopic/orthotopic nude mouse tumors. Exp Cell Res. 1994;213:156-63 pubmed
Skinner M, Raines E, Ross R. Dynamic expression of alpha 1 beta 1 and alpha 2 beta 1 integrin receptors by human vascular smooth muscle cells. Alpha 2 beta 1 integrin is required for chemotaxis across type I collagen-coated membranes. Am J Pathol. 1994;145:1070-81 pubmed
Wayner E, Carter W. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987;105:1873-84 pubmed
Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed

product information

Internal ID :

7742

Name :

P1H6

Depositor Name :

Wayner, E.A. / Carter, W.G.

Depositor Institution :

Fred Hutchison Cancer Research Center

Date Deposited :

10/11/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin alpha 2 (CD49b)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG3

Isotype for catalog (legacy) :

IgG3

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

2822727

Depositor Notes (Special Instructions) :

Immunostaining with frozen or acetone fixation. Doesn't work for FFPE. No function inhibition.

Collections :

CD antigens,Cell adhesion,Human

Search Keywords :

Wayner, E.A. / Carter, W.G., integrin alpha 2 (CD49b), Human, MIgG3, Human, ITGA2, BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2, AB_2619599, monoclonal, Cell adhesion/Cluster Determinant antigens/Human, Immunohistochemistry/Immunofluorescence/Western Blot/Immunoprecipitation/FACS

Antigen Molecular Weight :

Predicted: 129 kDa; Apparent: 150 kDa

Gene :

ITGA2

Alternate Gene Name(s) :

BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2

Uniprot ID :

P17301

Antibody Registry ID :

AB_2619599

Synonyms (Alternate Clone Names) :

CD49b

Immunogen :

Human HT-1080 fibrosarcoma cells

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Recommended Applications :

FACS,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

1691191 8623916

Immunofluorescence Pubmed IDs :

1691191 8421064

Western Blot Pubmed IDs :

8421064

Immunoprecipitation Pubmed IDs :

2822727 8421064 8020586

FACS Pubmed IDs :

7977639 8020586

Pubmed IDs :

8978611 10545505

Additional Information :

P1H6 recognizes an epitope that requires activation of the alpha2/beta1 complex.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell. Biol. 105, 1873-1884.; J. Cell Biol. 109(1), 397-407.; J Cell Biol 110, 1387-1404.; Cell 87, 1069-1078.; J. Cell Biol. 147, 619-629.; Methods in Enzymology 42, 117-153.

more info or order :

Developmental Studies Hybridoma Bank product webpage