fibronectin, cell binding domain | Developmental Studies Hybridoma Bank P1H11 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

fibronectin, cell binding domain

catalog :

P1H11

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1H11

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 7

Reference
Saito D, Kyakumoto S, Chosa N, Ibi M, Takahashi N, Okubo N, et al. Transforming growth factor-?1 induces epithelial-mesenchymal transition and integrin ?3?1-mediated cell migration of HSC-4 human squamous cell carcinoma cells through Slug. J Biochem. 2013;153:303-15 pubmed publisher
Yang J, Wang F, Tian L, Su J, Zhu X, Lin L, et al. Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface. Arch Virol. 2010;155:881-8 pubmed publisher
Arima Y, Inoue Y, Shibata T, Hayashi H, Nagano O, Saya H, et al. Rb depletion results in deregulation of E-cadherin and induction of cellular phenotypic changes that are characteristic of the epithelial-to-mesenchymal transition. Cancer Res. 2008;68:5104-12 pubmed publisher
Rojas R, Thomas J, Gehring A, Hill P, Belisle J, Harding C, et al. Phosphatidylinositol mannoside from Mycobacterium tuberculosis binds alpha5beta1 integrin (VLA-5) on CD4+ T cells and induces adhesion to fibronectin. J Immunol. 2006;177:2959-68 pubmed
Yu H, Frank D, Zhang J, You X, Carter W, Knudsen B. Basal prostate epithelial cells stimulate the migration of prostate cancer cells. Mol Carcinog. 2004;41:85-97 pubmed
Lampe P, Nguyen B, Gil S, Usui M, Olerud J, Takada Y, et al. Cellular interaction of integrin alpha3beta1 with laminin 5 promotes gap junctional communication. J Cell Biol. 1998;143:1735-47 pubmed
Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed

product information

Internal ID :

7930

Name :

P1H11

Depositor Name :

Wayner, E.A.

Depositor Institution :

Fred Hutchison Cancer Research Center

Date Deposited :

10/11/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

fibronectin, cell binding domain

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Human,Mouse,Primate,Rat

Species Tested (legacy) :

human, rat and primate

Initial Publication Pubmed ID :

1691191

Depositor Notes (Special Instructions) :

Frozen (acetone), paraformaldehyde, and methanol fixation work well for immunostaining. Doesn't appear to work for ELISA nor Flow cytometry. The MW observed by western blotting was 220 kDa (38 kDa and 190 kDa fibronectin A chain precursor). The epitope was mapped to the cell binding domain.

Collections :

Cell adhesion,Cell signaling,Extracellular matrix proteins,Human

Search Keywords :

Wayner, E.A., fibronectin, cell binding domain, Human, MIgG1, Human/Rat/Primate/mouse, FN1, FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2, AB_2619588, monoclonal, Human/Cell adhesion/Extracellular matrix proteins/Cell signaling, Immunohistochemistry/Immunofluorescence/FFPE/Western Blot/Immunoprecipitation

Antigen Molecular Weight :

Predicted: multiple isoforms between 218 and 256 kDa and one or two isoforms at 69-71 kDa, plus shorter cleavage products.

Gene :

FN1

Alternate Gene Name(s) :

FN, CIG, FNZ, MSF, ED-B, FINC, GFND, LETS, GFND2

Uniprot ID :

P02751

Antibody Registry ID :

AB_2619588

Immunogen :

Purified human fibronectin

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

Cell binding domain (aa 1267-1540 of isoform 1) [see Depositor Notes]

Recommended Applications :

FFPE,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunohistochemistry Pubmed IDs :

1691191 9852164

Immunofluorescence Pubmed IDs :

1691191

Western Blot Pubmed IDs :

18593909 23248240

Immunoprecipitation Pubmed IDs :

15378647

Additional Information :

Acetone or paraformaldehyde fixation

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 121(5), 1141-1152.; J. Immunol. 177, 2959-2968.; Hypertension 52, 910-917.; Cancer Res. 68(13), 5104-5112.; J. Card. Fail. 15(7), 616-628.Cancer Res. 70, 7810-7819.; Arch. Virol. 155(6), 881-888.

more info or order :

Developmental Studies Hybridoma Bank product webpage