integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor) | Developmental Studies Hybridoma Bank P1F6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor)

catalog :

P1F6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1F6

reactivity :

human, rat, chicken

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 13

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1a immunoprecipitation; human; loading ...; fig 1b	Beauvais D, Jung O, Yang Y, Sanderson R, Rapraeger A. Syndecan-1 (CD138) Suppresses Apoptosis in Multiple Myeloma by Activating IGF1 Receptor: Prevention by SynstatinIGF1R Inhibits Tumor Growth. Cancer Res. 2016;76:4981-93 pubmed publisher
	Buchholz D, Hikita S, Rowland T, Friedrich A, Hinman C, Johnson L, et al. Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells. Stem Cells. 2009;27:2427-34 pubmed publisher
	Scotton C, Krupiczojc M, Konigshoff M, Mercer P, Lee Y, Kaminski N, et al. Increased local expression of coagulation factor X contributes to the fibrotic response in human and murine lung injury. J Clin Invest. 2009;119:2550-63 pubmed publisher
	Wipff P, Rifkin D, Meister J, Hinz B. Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix. J Cell Biol. 2007;179:1311-23 pubmed
	Wang H, Kandel R. Chondrocytes attach to hyaline or calcified cartilage and bone. Osteoarthritis Cartilage. 2004;12:56-64 pubmed
	Akakura S, Singh S, Spataro M, Akakura R, Kim J, Albert M, et al. The opsonin MFG-E8 is a ligand for the alphavbeta5 integrin and triggers DOCK180-dependent Rac1 activation for the phagocytosis of apoptotic cells. Exp Cell Res. 2004;292:403-16 pubmed
	Pedchenko V, Zent R, Hudson B. Alpha(v)beta3 and alpha(v)beta5 integrins bind both the proximal RGD site and non-RGD motifs within noncollagenous (NC1) domain of the alpha3 chain of type IV collagen: implication for the mechanism of endothelia cell adhesion. J Biol Chem. 2004;279:2772-80 pubmed
	Zhou Q, Nakada M, Brooks P, Swenson S, Ritter M, Argounova S, et al. Contortrostatin, a homodimeric disintegrin, binds to integrin alphavbeta5. Biochem Biophys Res Commun. 2000;267:350-5 pubmed
	Memmo L, McKeown Longo P. The alphavbeta5 integrin functions as an endocytic receptor for vitronectin. J Cell Sci. 1998;111 ( Pt 4):425-33 pubmed
	Aplin J, Spanswick C, Behzad F, Kimber S, Vicovac L. Integrins beta 5, beta 3 and alpha v are apically distributed in endometrial epithelium. Mol Hum Reprod. 1996;2:527-34 pubmed
	De Nichilo M, Yamada K. Integrin alpha v beta 5-dependent serine phosphorylation of paxillin in cultured human macrophages adherent to vitronectin. J Biol Chem. 1996;271:11016-22 pubmed
	Weinacker A, Chen A, Agrez M, Cone R, Nishimura S, Wayner E, et al. Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor. J Biol Chem. 1994;269:6940-8 pubmed
	Wayner E, Orlando R, Cheresh D. Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface. J Cell Biol. 1991;113:919-29 pubmed

product information

Internal ID :

7934

Name :

P1F6

Depositor Name :

Wayner, E.A.

Depositor Institution :

Fred Hutchison Cancer Research Center

Date Deposited :

10/11/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1

Isotype for catalog (legacy) :

IgG1

Positive Tested Species Reactivity :

Chicken,Hamster,Human,Rat

Species Tested (legacy) :

human, rat and chicken

Initial Publication Pubmed ID :

1709170

Depositor Notes (Special Instructions) :

Immunostaining works only on acetone fixed preps. P1F6 completely inhibits alphaVbeta5 dependent binding to vitronectin-coated substrates. Doesn't cross-reactwith alphaVbeta3.

Collections :

CD antigens,Cell adhesion,Human

Search Keywords :

Wayner, E.A., integrin alpha V beta 5 (CD51, fibronectin/vitronectin receptor), Human, MIgG1, Human/Rat/Chicken/Hmaster, ITGAV/ITGB5, , AB_2619586, monoclonal, Cell adhesion/Cluster Determinant antigens/Human, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function Blocking/ELISA/FACS

Antigen Molecular Weight :

Apparent: 160 kDa (alpha chain), 95 kDa (beta chain)

Gene :

ITGAV/ITGB5

Uniprot ID :

P06756 P18084

Antibody Registry ID :

AB_2619586

Synonyms (Alternate Clone Names) :

CD49e, CD51

Immunogen :

small cell lung carcinoma from the UCLA P3 cell line expressing the vitronectin receptor.

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Recommended Applications :

ELISA,FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation

Immunoblotting (legacy) :

Immunohistochemistry Pubmed IDs :

19652365

Immunofluorescence Pubmed IDs :

18086923 9443892

Immunoprecipitation Pubmed IDs :

8120056 14610079

Function Blocking Pubmed IDs :

1709170 8120056 19658190 19652365 10623623 14697683 8631923

FACS Pubmed IDs :

8120056 10623623 14697347 8631923

Pubmed IDs :

9239663

Additional Information :

P1F6 recognizes a composite epitope on alphaV/beta5 native heterodimer integrin.

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell Biol. 133(4), 919-929.; J. Biol. Chem. 269(9), 6940-6948.; Mol. Hum. Reprod. 2, 527-534.; Biochem. Biophys. Res. Commun. 267(1), 350-355.; J. Cell Biol. 179(6), 1311-1323.; Stem Cells 27(10), 2427-2434.; J. Clin. Invest. 119(9), 2550-2563.

more info or order :

Developmental Studies Hybridoma Bank product webpage