product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin alpha 2 (CD49b)
catalog :
P1E6
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P1E6
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
citations: 18
Reference |
---|
Ozeki N, Hase N, Higuchi N, Hiyama T, Yamaguchi H, Kawai R, et al. Gelatin scaffold combined with bone morphogenetic protein-4 induces odontoblast-like cell differentiation involving integrin profile changes, autophagy-related gene 10, and Wnt5 sequentially in human induced pluripotent stem cells. Differentiation. 2017;93:1-14 pubmed publisher
|
Mori S, Kodaira M, Ito A, Okazaki M, Kawaguchi N, Hamada Y, et al. Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells. PLoS ONE. 2015;10:e0137486 pubmed publisher
|
Coelho N, González García C, Planell J, Salmerón Sánchez M, Altankov G. Different assembly of type IV collagen on hydrophilic and hydrophobic substrata alters endothelial cells interaction. Eur Cell Mater. 2010;19:262-72 pubmed
|
Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
|
Thorup A, Dabelsteen E, Schou S, Gil S, Carter W, Reibel J. Differential expression of integrins and laminin-5 in normal oral epithelia. APMIS. 1997;105:519-30 pubmed
|
Koyama H, Raines E, Bornfeldt K, Roberts J, Ross R. Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors. Cell. 1996;87:1069-78 pubmed
|
Niessen C, van der Raaij Helmer M, Hulsman E, van der Neut R, Jonkman M, Sonnenberg A. Deficiency of the integrin beta 4 subunit in junctional epidermolysis bullosa with pyloric atresia: consequences for hemidesmosome formation and adhesion properties. J Cell Sci. 1996;109 ( Pt 7):1695-706 pubmed
|
Tuckwell D, Calderwood D, Green L, Humphries M. Integrin alpha 2 I-domain is a binding site for collagens. J Cell Sci. 1995;108 ( Pt 4):1629-37 pubmed
|
Kamata T, Puzon W, Takada Y. Identification of putative ligand binding sites within I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29). J Biol Chem. 1994;269:9659-63 pubmed
|
Wayner E, Carter W. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987;105:1873-84 pubmed
|
Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed
|
Kaur P, Carter W. Integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts. J Cell Sci. 1992;103 ( Pt 3):755-63 pubmed
|
product information
Internal ID :
7782
Name :
P1E6
Depositor Name :
Wayner, E.A. / Carter, W.G.
Depositor Institution :
Fred Hutchison Cancer Research Center,
Date Deposited :
10/11/11
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
integrin alpha 2 (CD49b)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human
Initial Publication Pubmed ID :
2822727
Depositor Notes (Special Instructions) :
Inhibits binding to collagens I, III, IV and VI. Will IP alpha2 beta1 complex (non-reducing). Immunoblotting works under non-reducing conditions. Immunostaining with frozen or acetone fixation (not formalin). Doesn't work for FFPE.
Collections :
CD antigens,Cell adhesion,Human
Search Keywords :
Wayner, E.A. / Carter, W.G., integrin alpha 2 (CD49b), Human, MIgG1, Human, ITGA2, BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2, AB_2619597, monoclonal, Cell adhesion/Cluster Determinant antigens/Human, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation/Function Blocking/FACS
Antigen Molecular Weight :
Predicted: 129 kDa; Apparent: 150 kDa
Gene :
ITGA2
Alternate Gene Name(s) :
BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2
Uniprot ID :
P17301
Antibody Registry ID :
AB_2619597
Synonyms (Alternate Clone Names) :
CD49b
Immunogen :
Human HT-1080 fibrosarcoma cells
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1/FOX-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
I domain (aa 169-378 of Uniprot ID P17301-1)
Epitope Map PubMed ID :
7511592 7615681
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
1691191 1478970
Immunofluorescence Pubmed IDs :
1691191 9269298
Immunoprecipitation Pubmed IDs :
2822727
Function Blocking Pubmed IDs :
1691191 8978611 20460531 26408663 26988988
FACS Pubmed IDs :
8832392 27639333 26334633 24692545
Pubmed IDs :
19403697
Additional Information :
P1E6 recognizes an epitope that requires activation of the alpha2/beta1 complex.
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell. Biol. 105, 1873-1884.; J Cell Biol 110, 1387-1404.; J. Cell Biol. 137(6), 1445-1457.; Methods in Enzymology 426: 117-153.; Mol. Biol. Cell 20(13), 3012-3024.; Cancer Res 69(12), 4962-4968.; Eur. Cell Mater. 19, 262-272.; J Cell Physiol. 222(1), 156-167.; Cancer Res. 70, 4739-4748.
more info or order :
company information
Developmental Studies Hybridoma Bank
review
product type
related products
browse more products
questions and comments