product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin alpha 2 (CD49b)
catalog :
P1E6
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P1E6
reactivity :
human
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
citations: 18
Reference
Ozeki N, Hase N, Higuchi N, Hiyama T, Yamaguchi H, Kawai R, et al. Gelatin scaffold combined with bone morphogenetic protein-4 induces odontoblast-like cell differentiation involving integrin profile changes, autophagy-related gene 10, and Wnt5 sequentially in human induced pluripotent stem cells. Differentiation. 2017;93:1-14 pubmed publisher
Chen M, Lamar J, Li R, Hynes R, Kamm R. Elucidation of the Roles of Tumor Integrin ?1 in the Extravasation Stage of the Metastasis Cascade. Cancer Res. 2016;76:2513-24 pubmed publisher
El Azreq M, Arseneault C, Boisvert M, Pagé N, Allaeys I, Poubelle P, et al. Cooperation between IL-7 Receptor and Integrin α2β1 (CD49b) Drives Th17-Mediated Bone Loss. J Immunol. 2015;195:4198-209 pubmed publisher
Mori S, Kodaira M, Ito A, Okazaki M, Kawaguchi N, Hamada Y, et al. Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells. PLoS ONE. 2015;10:e0137486 pubmed publisher
Ozeki N, Mogi M, Yamaguchi H, Hiyama T, Kawai R, Hase N, et al. Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of ?1 integrin expression. J Biol Chem. 2014;289:14380-91 pubmed publisher
Coelho N, González García C, Planell J, Salmerón Sánchez M, Altankov G. Different assembly of type IV collagen on hydrophilic and hydrophobic substrata alters endothelial cells interaction. Eur Cell Mater. 2010;19:262-72 pubmed
Vincourt J, Etienne S, Cottet J, Delaunay C, Malanda C, Malanda B, et al. C-propeptides of procollagens I alpha 1 and II that differentially accumulate in enchondromas versus chondrosarcomas regulate tumor cell survival and migration. Cancer Res. 2010;70:4739-48 pubmed publisher
Bailey C, Kelly P, Casey P. Activation of Rap1 promotes prostate cancer metastasis. Cancer Res. 2009;69:4962-8 pubmed publisher
Araki E, Momota Y, Togo T, Tanioka M, Hozumi K, Nomizu M, et al. Clustering of syndecan-4 and integrin beta1 by laminin alpha 3 chain-derived peptide promotes keratinocyte migration. Mol Biol Cell. 2009;20:3012-24 pubmed publisher
Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
Thorup A, Dabelsteen E, Schou S, Gil S, Carter W, Reibel J. Differential expression of integrins and laminin-5 in normal oral epithelia. APMIS. 1997;105:519-30 pubmed
Koyama H, Raines E, Bornfeldt K, Roberts J, Ross R. Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors. Cell. 1996;87:1069-78 pubmed
Niessen C, van der Raaij Helmer M, Hulsman E, van der Neut R, Jonkman M, Sonnenberg A. Deficiency of the integrin beta 4 subunit in junctional epidermolysis bullosa with pyloric atresia: consequences for hemidesmosome formation and adhesion properties. J Cell Sci. 1996;109 ( Pt 7):1695-706 pubmed
Tuckwell D, Calderwood D, Green L, Humphries M. Integrin alpha 2 I-domain is a binding site for collagens. J Cell Sci. 1995;108 ( Pt 4):1629-37 pubmed
Kamata T, Puzon W, Takada Y. Identification of putative ligand binding sites within I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29). J Biol Chem. 1994;269:9659-63 pubmed
Wayner E, Carter W. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987;105:1873-84 pubmed
Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed
Kaur P, Carter W. Integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts. J Cell Sci. 1992;103 ( Pt 3):755-63 pubmed
product information
Internal ID :
7782
Name :
P1E6
Depositor Name :
Wayner, E.A. / Carter, W.G.
Depositor Institution :
Fred Hutchison Cancer Research Center,
Date Deposited :
10/11/11
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
integrin alpha 2 (CD49b)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human
Initial Publication Pubmed ID :
2822727
Depositor Notes (Special Instructions) :
Inhibits binding to collagens I, III, IV and VI. Will IP alpha2 beta1 complex (non-reducing). Immunoblotting works under non-reducing conditions. Immunostaining with frozen or acetone fixation (not formalin). Doesn't work for FFPE.
Collections :
CD antigens,Cell adhesion,Human
Search Keywords :
Wayner, E.A. / Carter, W.G., integrin alpha 2 (CD49b), Human, MIgG1, Human, ITGA2, BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2, AB_2619597, monoclonal, Cell adhesion/Cluster Determinant antigens/Human, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation/Function Blocking/FACS
Antigen Molecular Weight :
Predicted: 129 kDa; Apparent: 150 kDa
Gene :
ITGA2
Alternate Gene Name(s) :
BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2
Uniprot ID :
P17301
Antibody Registry ID :
AB_2619597
Synonyms (Alternate Clone Names) :
CD49b
Immunogen :
Human HT-1080 fibrosarcoma cells
Immunogen Sequence :
Full length protein
Clonality :
Monoclonal
Myeloma Strain :
NS-1/FOX-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
I domain (aa 169-378 of Uniprot ID P17301-1)
Epitope Map PubMed ID :
7511592 7615681
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
yes
Immunohistochemistry Pubmed IDs :
1691191 1478970
Immunofluorescence Pubmed IDs :
1691191 9269298
Immunoprecipitation Pubmed IDs :
2822727
Function Blocking Pubmed IDs :
1691191 8978611 20460531 26408663 26988988
FACS Pubmed IDs :
8832392 27639333 26334633 24692545
Pubmed IDs :
19403697
Additional Information :
P1E6 recognizes an epitope that requires activation of the alpha2/beta1 complex.
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell. Biol. 105, 1873-1884.; J Cell Biol 110, 1387-1404.; J. Cell Biol. 137(6), 1445-1457.; Methods in Enzymology 426: 117-153.; Mol. Biol. Cell 20(13), 3012-3024.; Cancer Res 69(12), 4962-4968.; Eur. Cell Mater. 19, 262-272.; J Cell Physiol. 222(1), 156-167.; Cancer Res. 70, 4739-4748.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US