integrin alpha-5 (CD49e) | Developmental Studies Hybridoma Bank P1D6 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

integrin alpha-5 (CD49e)

catalog :

P1D6

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

P1D6

reactivity :

African green monkey, human

application :

western blot, immunoprecipitation, blocking or activating experiments

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
immunoprecipitation; African green monkey; loading ...; fig 3d immunoprecipitation; human; loading ...; fig 3b	Varadaraj A, JENKINS L, Singh P, Chanda A, Snider J, Lee N, et al. TGF-Î² triggers rapid fibrillogenesis via a novel TÎ²RII-dependent fibronectin-trafficking mechanism. Mol Biol Cell. 2017;28:1195-1207 pubmed publisher
blocking or activating experiments; human; loading ...; fig 6C	Oudin M, Miller M, Klazen J, Kosciuk T, Lussiez A, Hughes S, et al. MenaINV mediates synergistic cross-talk between signaling pathways driving chemotaxis and haptotaxis. Mol Biol Cell. 2016;27:3085-3094 pubmed
	Oudin M, Jonas O, Kosciuk T, Broye L, Guido B, Wyckoff J, et al. Tumor Cell-Driven Extracellular Matrix Remodeling Drives Haptotaxis during Metastatic Progression. Cancer Discov. 2016;6:516-31 pubmed publisher
	Hooper S, Gaggioli C, Sahai E. A chemical biology screen reveals a role for Rab21-mediated control of actomyosin contractility in fibroblast-driven cancer invasion. Br J Cancer. 2010;102:392-402 pubmed publisher
	Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
	Edderkaoui M, Hong P, Vaquero E, Lee J, Fischer L, Friess H, et al. Extracellular matrix stimulates reactive oxygen species production and increases pancreatic cancer cell survival through 5-lipoxygenase and NADPH oxidase. Am J Physiol Gastrointest Liver Physiol. 2005;289:G1137-47 pubmed
	Brockbank E, Bridges J, Marshall C, Sahai E. Integrin beta1 is required for the invasive behaviour but not proliferation of squamous cell carcinoma cells in vivo. Br J Cancer. 2005;92:102-12 pubmed
	Kapila Y, Wang S, Johnson P. Mutations in the heparin binding domain of fibronectin in cooperation with the V region induce decreases in pp125(FAK) levels plus proteoglycan-mediated apoptosis via caspases. J Biol Chem. 1999;274:30906-13 pubmed
	Mould A, Askari J, Aota S, Yamada K, Irie A, Takada Y, et al. Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subu. J Biol Chem. 1997;272:17283-92 pubmed
	Wayner E, Carter W, Piotrowicz R, Kunicki T. The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platele. J Cell Biol. 1988;107:1881-91 pubmed
	Wayner E, Garcia Pardo A, Humphries M, McDonald J, Carter W. Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J Cell Biol. 1989;109:1321-30 pubmed
	Carter W, Kaur P, Gil S, Gahr P, Wayner E. Distinct functions for integrins alpha 3 beta 1 in focal adhesions and alpha 6 beta 4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J Cell Biol. 1990;111:3141-54 pubmed

product information

Internal ID :

7746

Name :

P1D6

Depositor Name :

Wayner, E.A. / Carter, W.G.

Depositor Institution :

Fred Hutchison Cancer Research Center,

Date Deposited :

10/11/11

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

integrin alpha-5 (CD49e)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG3

Isotype for catalog (legacy) :

IgG3

Positive Tested Species Reactivity :

Human

Species Tested (legacy) :

human

Initial Publication Pubmed ID :

2846588

Depositor Notes (Special Instructions) :

P1D6 recognizes native protein only. This antibody works well with formalin, 4 % paraformadehyde, acetone-methanol or ice-cold methanol fixation.

Collections :

CD antigens,Cell adhesion,Human

Search Keywords :

Wayner, E.A. / Carter, W.G. , integrin alpha-5 (CD49e) , Human, MIgG3, Human, ITGA5, CD49e, VLA5, FNRA, AB_2619596, Monoclonal, Human/Cell adhesion/Cluster determinant antigens, Western blot/Function blocking/ Immunoprecipitation/FFPE

Antigen Molecular Weight :

115 kDa

Gene :

ITGA5

Alternate Gene Name(s) :

CD49e, VLA5, FNRA

Uniprot ID :

P08648

Antibody Registry ID :

AB_2619596

Synonyms (Alternate Clone Names) :

CD49e

Immunogen :

Activated human killer T-lymphocytes (LAK)

Clonality :

Monoclonal

Myeloma Strain :

NS-1/FOX-NY

Epitope Mapped :

Yes

Epitope Location or Sequence :

non-contiguous amino acids in N-terminal repeats of alpha 5

Epitope Map PubMed ID :

9211865

Recommended Applications :

FFPE,Function Blocking,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

yes

Immunofluorescence Pubmed IDs :

2527858 2846588

Immunoprecipitation Pubmed IDs :

17697883

Function Blocking Pubmed IDs :

2846588 2269668 26811325 27559126 2527858 17697883 10521484

Additional Information :

P1D6 neutralizes binding of alpha5 beta 1 to the cell adhesion domain of fibronectin only. The antibody does not inhibit binding to collagen, laminin or vitronectin. RRID:AB_2619596

DSHB Growth Medium :

RPMI

References (legacy) :

J. Cell. Biol. 105, 1873-1884.; J. Cell Biol. 107(5), 1881-1891.; J. Cell Biol. 111, 3141-3154.; Am. J. Physiol. Gastrointest. Liver Physiol. 289, G1137-G1147.; Brit. J. Cancer 92(1), 102-112.; Methods in Enzymology 426, 117-153.; Br. J. Cancer 102(2), 392-402.

more info or order :

Developmental Studies Hybridoma Bank product webpage