product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
integrin alpha-3 (CD49c)
catalog :
P1B5
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
P1B5
reactivity :
human
application :
immunohistochemistry, immunocytochemistry, immunoprecipitation, FACS
more info or order :
Developmental Studies Hybridoma Bank product webpage
citations: 19
Reference
Verma R, Venkatareddy M, Kalinowski A, Patel S, Garg P. Integrin Ligation Results in Nephrin Tyrosine Phosphorylation In Vitro. PLoS ONE. 2016;11:e0148906 pubmed publisher
Wessels D, Lusche D, Kuhl S, Scherer A, Voss E, Soll D. Quantitative Motion Analysis in Two and Three Dimensions. Methods Mol Biol. 2016;1365:265-92 pubmed publisher
Scherer A, Kuhl S, Wessels D, Lusche D, Hanson B, Ambrose J, et al. A computer-assisted 3D model for analyzing the aggregation of tumorigenic cells reveals specialized behaviors and unique cell types that facilitate aggregate coalescence. PLoS ONE. 2015;10:e0118628 pubmed publisher
Yu M, Wang J, Muller D, Helenius J. In PC3 prostate cancer cells ephrin receptors crosstalk to β1-integrins to strengthen adhesion to collagen type I. Sci Rep. 2015;5:8206 pubmed publisher
Zhu G, Huang C, Qiu Z, Liu J, Zhang Z, Zhao N, et al. Expression and prognostic significance of CD151, c-Met, and integrin alpha3/alpha6 in pancreatic ductal adenocarcinoma. Dig Dis Sci. 2011;56:1090-8 pubmed publisher
Araki E, Momota Y, Togo T, Tanioka M, Hozumi K, Nomizu M, et al. Clustering of syndecan-4 and integrin beta1 by laminin alpha 3 chain-derived peptide promotes keratinocyte migration. Mol Biol Cell. 2009;20:3012-24 pubmed publisher
Kariya Y, Kato R, Itoh S, Fukuda T, Shibukawa Y, Sanzen N, et al. N-Glycosylation of laminin-332 regulates its biological functions. A novel function of the bisecting GlcNAc. J Biol Chem. 2008;283:33036-45 pubmed publisher
Wayner E, Hoffstrom B. Development of monoclonal antibodies to integrin receptors. Methods Enzymol. 2007;426:117-53 pubmed
Edick M, Tesfay L, Lamb L, Knudsen B, Miranti C. Inhibition of integrin-mediated crosstalk with epidermal growth factor receptor/Erk or Src signaling pathways in autophagic prostate epithelial cells induces caspase-independent death. Mol Biol Cell. 2007;18:2481-90 pubmed
Panicker A, Buhusi M, Erickson A, Maness P. Endocytosis of beta1 integrins is an early event in migration promoted by the cell adhesion molecule L1. Exp Cell Res. 2006;312:299-307 pubmed
Sadowski T, Dietrich S, Koschinsky F, Ludwig A, Proksch E, Titz B, et al. Matrix metalloproteinase 19 processes the laminin 5 gamma 2 chain and induces epithelial cell migration. Cell Mol Life Sci. 2005;62:870-80 pubmed
Brockbank E, Bridges J, Marshall C, Sahai E. Integrin beta1 is required for the invasive behaviour but not proliferation of squamous cell carcinoma cells in vivo. Br J Cancer. 2005;92:102-12 pubmed
Zhang X, Puzon McLaughlin W, Irie A, Kovach N, Prokopishyn N, Laferte S, et al. Alpha 3 beta 1 adhesion to laminin-5 and invasin: critical and differential role of integrin residues clustered at the boundary between alpha 3 N-terminal repeats 2 and 3. Biochemistry. 1999;38:14424-31 pubmed
Gronthos S, Stewart K, Graves S, Hay S, Simmons P. Integrin expression and function on human osteoblast-like cells. J Bone Miner Res. 1997;12:1189-97 pubmed
Wayner E, Gil S, Murphy G, Wilke M, Carter W. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes. J Cell Biol. 1993;121:1141-52 pubmed
Wayner E, Carter W, Piotrowicz R, Kunicki T. The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platele. J Cell Biol. 1988;107:1881-91 pubmed
Wayner E, Carter W. Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits. J Cell Biol. 1987;105:1873-84 pubmed
Wayner E, Garcia Pardo A, Humphries M, McDonald J, Carter W. Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J Cell Biol. 1989;109:1321-30 pubmed
Carter W, Wayner E, Bouchard T, Kaur P. The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells. J Cell Biol. 1990;110:1387-404 pubmed
product information
Internal ID :
7778
Name :
P1B5
Depositor Name :
Wayner, E.A. / Carter, W.G.
Depositor Institution :
Fred Hutchison Cancer Research Center,
Date Deposited :
10/11/11
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
integrin alpha-3 (CD49c)
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG1
Isotype for catalog (legacy) :
IgG1
Positive Tested Species Reactivity :
Human
Species Tested (legacy) :
human
Initial Publication Pubmed ID :
2846588
Depositor Notes (Special Instructions) :
P1B5 inhibits cell adhesion to collagen and fibronectin.
Collections :
CD antigens,Cell adhesion,Human
Search Keywords :
Wayner, E.A. / Carter, W.G. , ntegrin alpha-3 (CD49c) , Human, MIgG1, Human, ITGA3, CD49c, GAP-B3, MSK18, VCA-2, VLA3a, AB_2619595, Monoclonal, Human/Cell adhesion/Cluster determinant antigens, Immunohistochemistry/Immunofluorescence/Immunoprecipitation/Function blocking/Flow cytometry
Antigen Molecular Weight :
119 kDa
Gene :
ITGA3
Alternate Gene Name(s) :
CD49c, GAP-B3, MSK18, VCA-2, VLA3a
Uniprot ID :
P26006
Antibody Registry ID :
AB_2619595
Synonyms (Alternate Clone Names) :
CD49c
Immunogen :
HT1080 human fibrosarcoma cell line
Clonality :
Monoclonal
Myeloma Strain :
NS-1/FOX-NY
Epitope Mapped :
Yes
Epitope Location or Sequence :
NITVKN (75-80)
Epitope Map PubMed ID :
10572017
Recommended Applications :
FACS,Function Blocking,Immunofluorescence,Immunohistochemistry,Immunoprecipitation
Immunoblotting (legacy) :
yes
Immunofluorescence Pubmed IDs :
2846588
Western Blot Pubmed IDs :
2846588
Immunoprecipitation Pubmed IDs :
2527858 2822727 2846588 8501119 17697883
Function Blocking Pubmed IDs :
25644492 2822727 1691191 8501119 17697883 26848974 25790299 26498790 9258748
FACS Pubmed IDs :
10572017
Additional Information :
RRID:AB_2619595
DSHB Growth Medium :
RPMI
References (legacy) :
J. Cell. Biol. 105, 1873-1884.; J. Cell Biol. 107(5), 1881-1891.; J. Cell Biol. 110, 1387-1404.; J. Cell Biol. 121, 1141-1152.; Brit. J. Cancer 92(1), 102-112; Cell. Mol. Life Sci. 62(7-8), 870-880.; Exp. Cell Res. 312(3), 299-307.; Methods in Enzymology 426, 117-153.; Mol. Biol. Cell 18, 2481-2490.; J. Biol. Chem. 283(48), 33036-33045.; Mol. Biol. Cell 20(13), 3012-3024.; Dig. Dis. Sci. 56(4), 1090-1098.
more info or order :
Developmental Studies Hybridoma Bank product webpage
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US