Myosin heavy chain (human neonatal and adult fast fibers) | Developmental Studies Hybridoma Bank N3.36 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human neonatal and adult fast fibers)

catalog :

N3.36

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N3.36

reactivity :

human, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 17

Reference
Biondi O, Branchu J, Ben Salah A, Houdebine L, Bertin L, Chali F, et al. IGF-1R Reduction Triggers Neuroprotective Signaling Pathways in Spinal Muscular Atrophy Mice. J Neurosci. 2015;35:12063-79 pubmed publisher
Allwood M, Foster A, Arkell A, Beaudoin M, Snook L, Romanova N, et al. Respiratory muscle weakness in the Zucker diabetic fatty rat. Am J Physiol Regul Integr Comp Physiol. 2015;309:R780-7 pubmed publisher
Khodabukus A, Baehr L, Bodine S, Baar K. Role of contraction duration in inducing fast-to-slow contractile and metabolic protein and functional changes in engineered muscle. J Cell Physiol. 2015;230:2489-97 pubmed publisher
Khodabukus A, Baar K. Glucose concentration and streptomycin alter in vitro muscle function and metabolism. J Cell Physiol. 2015;230:1226-34 pubmed publisher
Luo Q, Douglas M, BURKHOLDER T, Sokoloff A. Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles. Muscle Nerve. 2014;49:534-44 pubmed publisher
Sieck D, Zhan W, Fang Y, Ermilov L, Sieck G, Mantilla C. Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions. Respir Physiol Neurobiol. 2012;180:88-96 pubmed publisher
Duris M, Picard B, Geay Y. Specificity of different anti-myosin heavy chain antibodies in bovine muscle. Meat Sci. 2000;55:67-78 pubmed
Biondi O, Grondard C, Lecolle S, Deforges S, Pariset C, Lopes P, et al. Exercise-induced activation of NMDA receptor promotes motor unit development and survival in a type 2 spinal muscular atrophy model mouse. J Neurosci. 2008;28:953-62 pubmed publisher
Brazelton T, Nystrom M, Blau H. Significant differences among skeletal muscles in the incorporation of bone marrow-derived cells. Dev Biol. 2003;262:64-74 pubmed
Dedkov E, Kostrominova T, Borisov A, Carlson B. MyoD and myogenin protein expression in skeletal muscles of senile rats. Cell Tissue Res. 2003;311:401-16 pubmed
Torgan C, Daniels M. Regulation of myosin heavy chain expression during rat skeletal muscle development in vitro. Mol Biol Cell. 2001;12:1499-508 pubmed
Maggs A, Taylor Harris P, Peckham M, Hughes S. Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J Muscle Res Cell Motil. 2000;21:101-13 pubmed
Shiotani A, Westra W, Flint P. Myosin heavy chain composition in human laryngeal muscles. Laryngoscope. 1999;109:1521-4 pubmed
Shiotani A, Jones R, Flint P. Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles. Ann Otol Rhinol Laryngol. 1999;108:509-15 pubmed
Cho M, Webster S, Blau H. Evidence for myoblast-extrinsic regulation of slow myosin heavy chain expression during muscle fiber formation in embryonic development. J Cell Biol. 1993;121:795-810 pubmed
Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed
Hughes S, Blau H. Muscle fiber pattern is independent of cell lineage in postnatal rodent development. Cell. 1992;68:659-71 pubmed

product information

Internal ID :

1304

Name :

N3.36

Depositor Name :

Blau, H.M.

Depositor Institution :

Baxter Lab for Stem Cell Biology, Stanford University

Depositor Website :

http://baxterlab.stanford.edu/

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human neonatal and adult fast fibers)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Bovine,Human,Rodent

Species Tested (legacy) :

human, rodent (others?)

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Fusion: 1983. Recognizes neonatal and adult fast fibers.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H. M., Myosin heavy chain (human neonatal and adult fast fibers), Human, MIgM, Human/Rodent/Bovine, MYH8, DA7; MyHC-pn; gtMHC-F; MyHC-peri, AB_528380, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Immunoprecipitation

Antigen Molecular Weight :

220kDa

Gene :

MYH8

Alternate Gene Name(s) :

DA7; MyHC-pn; gtMHC-F; MyHC-peri

Uniprot ID :

P13535

Antibody Registry ID :

AB_528380

Immunogen :

partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle

Clonality :

Monoclonal

Myeloma Strain :

SP2/2

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

23835800 10961835 1531450

Immunofluorescence Pubmed IDs :

22063925 22060906 10961835 8491773 26311784 11359938 12658448 18216203 14512018

Western Blot Pubmed IDs :

23835800 22060906 10335716 3342447 8491773 25857846 26246509 25358470 10499066

ELISA Pubmed IDs :

3342447

Additional Information :

This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Mol. Biol. Cell 12, 1499-1508.; Cell Tissue Res. 311, 401-416.; J. Neurosci. 28(4), 953-962.

more info or order :

Developmental Studies Hybridoma Bank product webpage