Myosin heavy chain (human all fibers except fast IIb) | Developmental Studies Hybridoma Bank N2.261 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human all fibers except fast IIb)

catalog :

N2.261

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N2.261

reactivity :

human, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 20

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; zebrafish ; fig 3	Sallin P, Jazwinska A. Acute stress is detrimental to heart regeneration in zebrafish. Open Biol. 2016;6: pubmed publisher
immunohistochemistry; human; 1:400; loading ...; tbl 1	Shah F, Berggren D, Holmlund T, Levring JÃ¤ghagen E, StÃ¥l P. Unique expression of cytoskeletal proteins in human soft palate muscles. J Anat. 2016;228:487-94 pubmed publisher
	Sokoloff A, Douglas M, Rahnert J, BURKHOLDER T, Easley K, Luo Q. Absence of morphological and molecular correlates of sarcopenia in the macaque tongue muscle styloglossus. Exp Gerontol. 2016;84:40-48 pubmed publisher
	Peng X, He Q, Li G, Ma J, Zhong T. Rac1-PAK2 pathway is essential for zebrafish heart regeneration. Biochem Biophys Res Commun. 2016;472:637-42 pubmed publisher
	Johnston A, Murphy K, Jenkinson L, Laine D, Emmrich K, Faou P, et al. Targeting of Fn14 Prevents Cancer-Induced Cachexia and Prolongs Survival. Cell. 2015;162:1365-78 pubmed publisher
	Boman N, BurÃ©n J, Antti H, Svensson M. Gene expression and fiber type variations in repeated vastus lateralis biopsies. Muscle Nerve. 2015;52:812-7 pubmed publisher
	Sallin P, de Preux Charles A, Duruz V, Pfefferli C, JaÅºwiÅ„ska A. A dual epimorphic and compensatory mode of heart regeneration in zebrafish. Dev Biol. 2015;399:27-40 pubmed publisher
	Gliemann L, Olesen J, Biensø R, Schmidt J, Akerstrom T, Nyberg M, et al. Resveratrol modulates the angiogenic response to exercise training in skeletal muscles of aged men. Am J Physiol Heart Circ Physiol. 2014;307:H1111-9 pubmed publisher
	Luo Q, Douglas M, BURKHOLDER T, Sokoloff A. Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles. Muscle Nerve. 2014;49:534-44 pubmed publisher
	Gruenbaum Cohen Y, Harel I, Umansky K, Tzahor E, Snapper S, Shilo B, et al. The actin regulator N-WASp is required for muscle-cell fusion in mice. Proc Natl Acad Sci U S A. 2012;109:11211-6 pubmed publisher
	Schaart G, Hesselink R, Keizer H, Van Kranenburg G, Drost M, Hesselink M. A modified PAS stain combined with immunofluorescence for quantitative analyses of glycogen in muscle sections. Histochem Cell Biol. 2004;122:161-9 pubmed
	Liu J, Thornell L, Pedrosa Domellöf F. Muscle spindles in the deep muscles of the human neck: a morphological and immunocytochemical study. J Histochem Cytochem. 2003;51:175-86 pubmed
	Lindman R, Stål P. Abnormal palatopharyngeal muscle morphology in sleep-disordered breathing. J Neurol Sci. 2002;195:11-23 pubmed
	Liu J, Eriksson P, Thornell L, Pedrosa Domellöf F. Myosin heavy chain composition of muscle spindles in human biceps brachii. J Histochem Cytochem. 2002;50:171-83 pubmed
	Lindman R, Paulin G, Stål P. Morphological characterization of the levator veli palatini muscle in children born with cleft palates. Cleft Palate Craniofac J. 2001;38:438-48 pubmed
	Jergovic D, Stål P, Lidman D, Lindvall B, Hildebrand C. Changes in a rat facial muscle after facial nerve injury and repair. Muscle Nerve. 2001;24:1202-12 pubmed
	Stål P, Lindman R. Characterisation of human soft palate muscles with respect to fibre types, myosins and capillary supply. J Anat. 2000;197 ( Pt 2):275-90 pubmed
	Pedrosa Domellof F, Holmgren Y, Lucas C, Hoh J, Thornell L. Human extraocular muscles: unique pattern of myosin heavy chain expression during myotube formation. Invest Ophthalmol Vis Sci. 2000;41:1608-16 pubmed
	Hughes S, Cho M, Karsch Mizrachi I, Travis M, Silberstein L, Leinwand L, et al. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle. Dev Biol. 1993;158:183-99 pubmed
	Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed

product information

Internal ID :

1298

Name :

N2.261

Depositor Name :

Blau, H.M.

Depositor Institution :

Stanford University School of Medicine

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human all fibers except fast IIb)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgG1, kappa light chain

Isotype for catalog (legacy) :

IgG1, kappa light chain

Positive Tested Species Reactivity :

Human,Rodent,Zebrafish

Species Tested (legacy) :

human, rodent (others?)

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Fusion: 1983.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H.M., Myosin heavy chain (human all fibers except fast IIb), Human, MIgG1, kappa light chain , Human/rodent/zebrafish, MYH7, CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB, AB_531790, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA

Antigen Molecular Weight :

220kDa

Gene :

MYH7

Alternate Gene Name(s) :

CMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB

Uniprot ID :

P12883

Antibody Registry ID :

AB_531790

Synonyms (Alternate Clone Names) :

Anti-MyHCI/3rd+IIa

Immunogen :

partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle

Immunogen Sequence :

Full length protein

Clonality :

Monoclonal

Myeloma Strain :

SP2/2

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal first 100 aa

Epitope Map PubMed ID :

7687223

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

7687223 10845576 11005719 11522165 11494274 11867069 11799136 12533526 25128170

Immunofluorescence Pubmed IDs :

3342447 7687223 15322861 25557620 26966072 26359988 25703336

Western Blot Pubmed IDs :

7687223 11005719 11522165 11867069 11799136 22736793 27566374 23835800

ELISA Pubmed IDs :

7687223

Pubmed IDs :

25128170

Additional Information :

Stains MyHCIIa and third developmental stage MyHCI, and the epitope on slow MHC is different from that of mAbs A4.951 and A4.951 [PMID 7687223]. Stains all fibers except fast IIb [PMID 3342447; PMID 11005719]. This antibody may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive. RRID: AB_531790

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Invest. Ophthalmol. Vis. Sci. 41(7), 1608-1616.; J. Anat. 197, 275-290.; Cleft Palate-Craniofacial J. 38(5), 438-448.; Muscle Nerve 24, 1202-1212.; J. Neurol. Sci. 195, 11-23.; J. Histochem. Cytochem. 50(2), 171-183.; J. Histochem. Cytochem. 51(2), 175-186.; Histochem. Cell Biol. 122, 161-169.; PNAS doi: 10.1073/pnas.1116065109.; Am J. Physiol. Heart Circ. Physiol. doi:10.1152/ajpheart.00168.2014.

more info or order :

Developmental Studies Hybridoma Bank product webpage