Myosin heavy chain (human neonatal fast IIa fibers) | Developmental Studies Hybridoma Bank N1.551 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

Myosin heavy chain (human neonatal fast IIa fibers)

catalog :

N1.551

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

N1.551

reactivity :

human, cow

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 12

Reference
Luo Q, Douglas M, BURKHOLDER T, Sokoloff A. Absence of developmental and unconventional myosin heavy chain in human suprahyoid muscles. Muscle Nerve. 2014;49:534-44 pubmed publisher
Sieck D, Zhan W, Fang Y, Ermilov L, Sieck G, Mantilla C. Structure-activity relationships in rodent diaphragm muscle fibers vs. neuromuscular junctions. Respir Physiol Neurobiol. 2012;180:88-96 pubmed publisher
Duris M, Picard B, Geay Y. Specificity of different anti-myosin heavy chain antibodies in bovine muscle. Meat Sci. 2000;55:67-78 pubmed
Zhou Y, Liu D, Kaminski H. Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle. J Physiol. 2011;589:4601-14 pubmed publisher
Lee Y, Mikesh M, Smith I, Rimer M, Thompson W. Muscles in a mouse model of spinal muscular atrophy show profound defects in neuromuscular development even in the absence of failure in neuromuscular transmission or loss of motor neurons. Dev Biol. 2011;356:432-44 pubmed publisher
Zhou Y, Liu D, Kaminski H. Myosin heavy chain expression in mouse extraocular muscle: more complex than expected. Invest Ophthalmol Vis Sci. 2010;51:6355-63 pubmed publisher
Salerno M, Thomas M, Forbes D, Watson T, Kambadur R, Sharma M. Molecular analysis of fiber type-specific expression of murine myostatin promoter. Am J Physiol Cell Physiol. 2004;287:C1031-40 pubmed
Mousavi K, Parry D, Jasmin B. BDNF rescues myosin heavy chain IIB muscle fibers after neonatal nerve injury. Am J Physiol Cell Physiol. 2004;287:C22-9 pubmed
Barresi M, D Angelo J, Hernández L, Devoto S. Distinct mechanisms regulate slow-muscle development. Curr Biol. 2001;11:1432-8 pubmed
Shiotani A, Jones R, Flint P. Postnatal development of myosin heavy chain isoforms in rat laryngeal muscles. Ann Otol Rhinol Laryngol. 1999;108:509-15 pubmed
Tikunov B, Levine S, Mancini D. Chronic congestive heart failure elicits adaptations of endurance exercise in diaphragmatic muscle. Circulation. 1997;95:910-6 pubmed
Webster C, Silberstein L, Hays A, Blau H. Fast muscle fibers are preferentially affected in Duchenne muscular dystrophy. Cell. 1988;52:503-13 pubmed

product information

Internal ID :

1292

Name :

N1.551

Depositor Name :

Blau, H.M.

Depositor Institution :

Baxter Lab for Stem Cell Biology, Stanford University

Depositor Website :

http://baxterlab.stanford.edu/

Date Deposited :

8/25/93

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

Myosin heavy chain (human neonatal fast IIa fibers)

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Bovine,Human,Rodent,Zebrafish

Species Tested (legacy) :

rodent, zebrafish

Initial Publication Pubmed ID :

3342447

Depositor Notes (Special Instructions) :

Fusion: 1983. Recognizes IIA fibers in human. In rat, high affinity for IIA fibers, low affinity for IIX fibers, and doesn't stain IIB.

Collections :

Cardiac development,Cell markers,Cytoskeleton,Human,Skeletal muscle

Search Keywords :

Blau, H. M., Myosin heavy chain (human neonatal fast IIa fibers), Human, MIgM, Human/Rodent/Zebrafish/Bovine, MYH2, BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa, AB_2147780, monoclonal, Human/Cell markers/Cytoskeletal elements/Skeletal muscle/Cardiac development proteins, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Immunoprecipitation/n1551

Antigen Molecular Weight :

220kDa

Gene :

MYH2

Alternate Gene Name(s) :

BM3; MYH2A; MYPOP; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa

Uniprot ID :

Q9UKX2

Antibody Registry ID :

AB_2147780

Immunogen :

partially purified myosin (pyrophosphate extracted) from human neonatal (5 days old) skeletal muscle

Clonality :

Monoclonal

Myeloma Strain :

SP2/2

Epitope Mapped :

Recommended Applications :

ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

23835800 14973145

Immunofluorescence Pubmed IDs :

3342447 22063925 22060906 11566102 15189813 21727215 20610840 21658376

Western Blot Pubmed IDs :

3342447 23835800 22060906 10335716 9054750

ELISA Pubmed IDs :

3342447

Pubmed IDs :

11566102

Additional Information :

This antibody has been reported to also bind neonatal MyHC. It may recognize more than one myosin isoform depending on the tissue, developmental stage, or species. The gene, Uniprot ID and gene ID associated with this mAb are not exclusive.

DSHB Growth Medium :

Iscove's

References (legacy) :

Cell 52, 503-513.; Cell 68, 659-671.; J. Cell Biol. 121, 795-810.; Dev. Biol. 158, 183-199.; Am. J. Physiol. Cell Physiol. 287, C1031-C1040.

more info or order :

Developmental Studies Hybridoma Bank product webpage