product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
keratan sulfate
catalog :
MZ15
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MZ15
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry
more info or order :
citations: 11
| Reference |
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Davis R, Kirschner M. The fate of cells in the tailbud of Xenopus laevis. Development. 2000;127:255-67 pubmed
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Bayliss M, Osborne D, Woodhouse S, Davidson C. Sulfation of chondroitin sulfate in human articular cartilage. The effect of age, topographical position, and zone of cartilage on tissue composition. J Biol Chem. 1999;274:15892-900 pubmed
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Zanetti M, Ratcliffe A, Watt F. Two subpopulations of differentiated chondrocytes identified with a monoclonal antibody to keratan sulfate. J Cell Biol. 1985;101:53-9 pubmed
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Watt F. Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture. J Cell Sci. 1988;89 ( Pt 3):373-8 pubmed
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Craig F, Ralphs J, Bentley G, Archer C. MZ15, a monoclonal antibody recognizing keratan sulphate, stains chick tendon. Histochem J. 1987;19:651-7 pubmed
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Craig F, Bentley G, Archer C. The spatial and temporal pattern of collagens I and II and keratan sulphate in the developing chick metatarsophalangeal joint. Development. 1987;99:383-91 pubmed
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Mehmet H, Scudder P, Tang P, Hounsell E, Caterson B, Feizi T. The antigenic determinants recognized by three monoclonal antibodies to keratan sulphate involve sulphated hepta- or larger oligosaccharides of the poly(N-acetyllactosamine) series. Eur J Biochem. 1986;157:385-91 pubmed
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Allard S, Bayliss M, Maini R. The synovium-cartilage junction of the normal human knee. Implications for joint destruction and repair. Arthritis Rheum. 1990;33:1170-9 pubmed
|
Siczkowski M, Watt F. Subpopulations of chondrocytes from different zones of pig articular cartilage. Isolation, growth and proteoglycan synthesis in culture. J Cell Sci. 1990;97 ( Pt 2):349-60 pubmed
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product information
Internal ID :
1040
Name :
MZ15
Depositor Name :
Watt, F.M.
Depositor Institution :
Cambridge Research Institute
Date Deposited :
2/20/07
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
keratan sulfate
Antigen Species :
Pig
Host Species :
mouse
Isotype :
MIgG1, kappa light chain
Isotype for catalog (legacy) :
IgG1, kappa light chain
Positive Tested Species Reactivity :
Bovine,Chicken,Fish,Human,Mouse,Pig,Rabbit,Xenopus
Species Tested (legacy) :
human, pig, rabbit, dog, chick, and mouse
Initial Publication Pubmed ID :
3159736
Depositor Notes (Special Instructions) :
Keratan sulphate proteoglycan competes with 125-I-proteoglycan monomer for MZ15 binding.
Collections :
Extracellular matrix proteins,Human,Xenopus
Search Keywords :
Watt, F.M., keratan sulfate, Pig, MIgG1, kappa light chain, Human/Pig/Rabbit/chicken/Mouse/Xenopus/Bovine/Fish, , , AB_760352, monoclonal, Extracellular matrix proteins/Human/Xenopus, ELISA/Immunofluorescence/Immunohistochemistry/Western Blot/FFPE
Antibody Registry ID :
AB_760352
Immunogen :
Chondrocytes from larynx xartilage of 9 Month old pigs.
Clonality :
Monoclonal
Myeloma Strain :
NS1
Epitope Mapped :
Yes
Epitope Location or Sequence :
The antigenic determinant recognized by MZ15 is part of the keratan sulfate chain that is sensitive to keratanase digestion and require the sulfate residues.
Epitope Map PubMed ID :
3159736 2423332 2430799
Recommended Applications :
ELISA,FFPE,Immunofluorescence,Immunohistochemistry,Western Blot
Immunohistochemistry Pubmed IDs :
26014913 2390122 10603344
Immunofluorescence Pubmed IDs :
3159736 22232554 2958266 3058725 2965117 2277096
Western Blot Pubmed IDs :
10336494 2277096
ELISA Pubmed IDs :
3159736
FFPE Pubmed IDs :
2390122
Additional Information :
MZ15 recognizes both skeletal and corneal keratan sulfate.
DSHB Growth Medium :
Iscove's
References (legacy) :
J. Cell Biol. 101, 53-59.; J. Biol. Chem. 287(10), 7427-7435.
more info or order :
company information
Developmental Studies Hybridoma Bank