product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Huntingtin
catalog :
MW8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MW8
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
citations: 19
Published Application/Species/Sample/Dilution | Reference |
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Zheng S, Ghitani N, Blackburn J, Liu J, Zeitlin S. A series of N-terminal epitope tagged Hdh knock-in alleles expressing normal and mutant huntingtin: their application to understanding the effect of increasing the length of normal Huntingtin's polyglutamine stretch on CAG140 mouse model pathogenesis. Mol Brain. 2012;5:28 pubmed publisher
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Sontag E, Lotz G, Agrawal N, Tran A, Aron R, Yang G, et al. Methylene blue modulates huntingtin aggregation intermediates and is protective in Huntington's disease models. J Neurosci. 2012;32:11109-19 pubmed
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Wanderer J, Morton A. Differential morphology and composition of inclusions in the R6/2 mouse and PC12 cell models of Huntington's disease. Histochem Cell Biol. 2007;127:473-84 pubmed
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Khoshnan A, Ko J, Patterson P. Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity. Proc Natl Acad Sci U S A. 2002;99:1002-7 pubmed
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Ko J, Ou S, Patterson P. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Res Bull. 2001;56:319-29 pubmed
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product information
Internal ID :
4005
Name :
MW8
Depositor Name :
Patterson, P.H.
Depositor Institution :
California Institute of Technology
Date Deposited :
1/23/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Huntingtin
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Mouse
Species Tested (legacy) :
mouse and human
Initial Publication Pubmed ID :
11719267
Depositor Notes (Special Instructions) :
The MW8 exclusively stains aggregates as inclusions in both nuclei and in the neuropil. This antigen is also known as huntington disease protein.
Collections :
Human,Nucleus
Search Keywords :
Paul H. Patterson, Huntingtin, Human , MIgG2a, Mouse/Human, HTT, HD, IT15, AB_528297, Monoclonal, Human/Nucleus, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation
Antigen Molecular Weight :
347.6 kDa
Gene :
HTT
Alternate Gene Name(s) :
HD, IT15
Uniprot ID :
P42858
Antibody Registry ID :
AB_528297
Immunogen :
Hd exon-1 67Q aggregate
Immunogen Sequence :
HDx-67Q
Clonality :
Monoclonal
Myeloma Strain :
HL-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
AEEPLHRPK/ a.a. 83-90
Epitope Map PubMed ID :
11719267
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
?
Immunohistochemistry Pubmed IDs :
11719267 20140187 22892315 22875942 26436900 27196694
Immunofluorescence Pubmed IDs :
11792860 24086450 22984513 20140187 19491400 17285342 22037470 20086007
Western Blot Pubmed IDs :
20140187 22365609 22984513 19491400 20086007
Immunoprecipitation Pubmed IDs :
22365609 22375012 20086007
ELISA Pubmed IDs :
11719267
Pubmed IDs :
28537272
Additional Information :
RRID:AB_528297; Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW8 to assay huntingtin aggregates. PMID: 22365609 22984513
DSHB Growth Medium :
Iscove's
References (legacy) :
Brain Res. Bull. 56(3-4), 319-329.; Proc. Natl. Acad. Sci. USA 99(2), 1002-1007.; PLoS Genet 6(2): e1000838. doi:10.1371/journal.pgen.1000838.
more info or order :
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