product summary
company name :
Developmental Studies Hybridoma Bank
product type :
antibody
product name :
Huntingtin
catalog :
MW8
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MW8
reactivity :
human, mouse
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
citations: 19
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:10; loading ...; fig 5
Back M, Kurzawa J, Ruggieri S, von Engelhardt J. C57BL/6 Background Attenuates mHTT Toxicity in the Striatum of YAC128 Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 4i
Victor M, Richner M, Olsen H, Lee S, Monteys A, Ma C, et al. Striatal neurons directly converted from Huntington's disease patient fibroblasts recapitulate age-associated disease phenotypes. Nat Neurosci. 2018;21:341-352 pubmed publisher
  • immunohistochemistry; mouse; 1:750; fig 4a
Bragg R, Coffey S, Weston R, Ament S, Cantle J, Minnig S, et al. Motivational, proteostatic and transcriptional deficits precede synapse loss, gliosis and neurodegeneration in the B6.HttQ111/+ model of Huntington's disease. Sci Rep. 2017;7:41570 pubmed publisher
Lin H, Boatz J, Krabbendam I, Kodali R, Hou Z, Wetzel R, et al. Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core. Nat Commun. 2017;8:15462 pubmed publisher
Bayram Weston Z, Jones L, Dunnett S, Brooks S. Comparison of mHTT Antibodies in Huntington's Disease Mouse Models Reveal Specific Binding Profiles and Steady-State Ubiquitin Levels with Disease Development. PLoS ONE. 2016;11:e0155834 pubmed publisher
Liu W, Kohn J, Szwed S, Pariser E, Sepe S, Haripal B, et al. Human mutant huntingtin disrupts vocal learning in transgenic songbirds. Nat Neurosci. 2015;18:1617-22 pubmed publisher
Young D, Mayer F, Vidotto N, Schweizer T, Berth R, Abramowski D, et al. Mutant huntingtin gene-dose impacts on aggregate deposition, DARPP32 expression and neuroinflammation in HdhQ150 mice. PLoS ONE. 2013;8:e75108 pubmed publisher
Marcellin D, Abramowski D, Young D, Richter J, Weiss A, Marcel A, et al. Fragments of HdhQ150 mutant huntingtin form a soluble oligomer pool that declines with aggregate deposition upon aging. PLoS ONE. 2012;7:e44457 pubmed publisher
Zheng S, Ghitani N, Blackburn J, Liu J, Zeitlin S. A series of N-terminal epitope tagged Hdh knock-in alleles expressing normal and mutant huntingtin: their application to understanding the effect of increasing the length of normal Huntingtin's polyglutamine stretch on CAG140 mouse model pathogenesis. Mol Brain. 2012;5:28 pubmed publisher
Sontag E, Lotz G, Agrawal N, Tran A, Aron R, Yang G, et al. Methylene blue modulates huntingtin aggregation intermediates and is protective in Huntington's disease models. J Neurosci. 2012;32:11109-19 pubmed
Sapp E, Valencia A, Li X, Aronin N, Kegel K, Vonsattel J, et al. Native mutant huntingtin in human brain: evidence for prevalence of full-length monomer. J Biol Chem. 2012;287:13487-99 pubmed publisher
Baldo B, Paganetti P, Grueninger S, Marcellin D, Kaltenbach L, Lo D, et al. TR-FRET-based duplex immunoassay reveals an inverse correlation of soluble and aggregated mutant huntingtin in huntington's disease. Chem Biol. 2012;19:264-75 pubmed publisher
Miller J, Arrasate M, Brooks E, Libeu C, Legleiter J, Hatters D, et al. Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nat Chem Biol. 2011;7:925-34 pubmed publisher
Zheng S, Clabough E, Sarkar S, Futter M, Rubinsztein D, Zeitlin S. Deletion of the huntingtin polyglutamine stretch enhances neuronal autophagy and longevity in mice. PLoS Genet. 2010;6:e1000838 pubmed publisher
Landles C, Sathasivam K, Weiss A, Woodman B, Moffitt H, Finkbeiner S, et al. Proteolysis of mutant huntingtin produces an exon 1 fragment that accumulates as an aggregated protein in neuronal nuclei in Huntington disease. J Biol Chem. 2010;285:8808-23 pubmed publisher
Legleiter J, Lotz G, Miller J, Ko J, Ng C, Williams G, et al. Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. J Biol Chem. 2009;284:21647-58 pubmed publisher
Wanderer J, Morton A. Differential morphology and composition of inclusions in the R6/2 mouse and PC12 cell models of Huntington's disease. Histochem Cell Biol. 2007;127:473-84 pubmed
Khoshnan A, Ko J, Patterson P. Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity. Proc Natl Acad Sci U S A. 2002;99:1002-7 pubmed
Ko J, Ou S, Patterson P. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Res Bull. 2001;56:319-29 pubmed
product information
Internal ID :
4005
Name :
MW8
Depositor Name :
Patterson, P.H.
Depositor Institution :
California Institute of Technology
Date Deposited :
1/23/03
Allow Hybridoma Distribution :
Yes
Cells Available (legacy) :
Yes
Antigen :
Huntingtin
Antigen Species :
Human
Host Species :
mouse
Isotype :
MIgG2a
Isotype for catalog (legacy) :
IgG2a
Positive Tested Species Reactivity :
Human,Mouse
Species Tested (legacy) :
mouse and human
Initial Publication Pubmed ID :
11719267
Depositor Notes (Special Instructions) :
The MW8 exclusively stains aggregates as inclusions in both nuclei and in the neuropil. This antigen is also known as huntington disease protein.
Collections :
Human,Nucleus
Search Keywords :
Paul H. Patterson, Huntingtin, Human , MIgG2a, Mouse/Human, HTT, HD, IT15, AB_528297, Monoclonal, Human/Nucleus, Immunohistochemistry/Immunofluorescence/Western blot/Immunoprecipitation
Antigen Molecular Weight :
347.6 kDa
Gene :
HTT
Alternate Gene Name(s) :
HD, IT15
Uniprot ID :
P42858
Antibody Registry ID :
AB_528297
Immunogen :
Hd exon-1 67Q aggregate
Immunogen Sequence :
HDx-67Q
Clonality :
Monoclonal
Myeloma Strain :
HL-1
Epitope Mapped :
Yes
Epitope Location or Sequence :
AEEPLHRPK/ a.a. 83-90
Epitope Map PubMed ID :
11719267
Recommended Applications :
ELISA,Immunofluorescence,Immunohistochemistry,Immunoprecipitation,Western Blot
Immunoblotting (legacy) :
?
Immunohistochemistry Pubmed IDs :
11719267 20140187 22892315 22875942 26436900 27196694
Immunofluorescence Pubmed IDs :
11792860 24086450 22984513 20140187 19491400 17285342 22037470 20086007
Western Blot Pubmed IDs :
20140187 22365609 22984513 19491400 20086007
Immunoprecipitation Pubmed IDs :
22365609 22375012 20086007
ELISA Pubmed IDs :
11719267
Pubmed IDs :
28537272
Additional Information :
RRID:AB_528297; Time-resolved Förster resonance energy transfer (TR-FRET) assay has been developed using MW8 to assay huntingtin aggregates. PMID: 22365609 22984513
DSHB Growth Medium :
Iscove's
References (legacy) :
Brain Res. Bull. 56(3-4), 319-329.; Proc. Natl. Acad. Sci. USA 99(2), 1002-1007.; PLoS Genet 6(2): e1000838. doi:10.1371/journal.pgen.1000838.
company information
Developmental Studies Hybridoma Bank
University of Iowa
http://dshb.biology.uiowa.edu
headquarters: US