huntingtin | Developmental Studies Hybridoma Bank MW7 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

huntingtin

catalog :

MW7

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MW7

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:100	Cicchetti F, Lacroix S, Cisbani G, Vallières N, Saint Pierre M, St Amour I, et al. Mutant huntingtin is present in neuronal grafts in Huntington disease patients. Ann Neurol. 2014;76:31-42 pubmed publisher
	Lin H, Boatz J, Krabbendam I, Kodali R, Hou Z, Wetzel R, et al. Fibril polymorphism affects immobilized non-amyloid flanking domains of huntingtin exon1 rather than its polyglutamine core. Nat Commun. 2017;8:15462 pubmed publisher
	Jeon I, Cicchetti F, Cisbani G, Lee S, Li E, Bae J, et al. Human-to-mouse prion-like propagation of mutant huntingtin protein. Acta Neuropathol. 2016;132:577-92 pubmed publisher
	Liu W, Kohn J, Szwed S, Pariser E, Sepe S, Haripal B, et al. Human mutant huntingtin disrupts vocal learning in transgenic songbirds. Nat Neurosci. 2015;18:1617-22 pubmed publisher
	Miller J, Arrasate M, Brooks E, Libeu C, Legleiter J, Hatters D, et al. Identifying polyglutamine protein species in situ that best predict neurodegeneration. Nat Chem Biol. 2011;7:925-34 pubmed publisher
	Hands S, Sajjad M, Newton M, Wyttenbach A. In vitro and in vivo aggregation of a fragment of huntingtin protein directly causes free radical production. J Biol Chem. 2011;286:44512-20 pubmed publisher
	Zhang Q, Yeh T, Leyva A, Frank L, Miller J, Kim Y, et al. A compact beta model of huntingtin toxicity. J Biol Chem. 2011;286:8188-96 pubmed publisher
	Legleiter J, Lotz G, Miller J, Ko J, Ng C, Williams G, et al. Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. J Biol Chem. 2009;284:21647-58 pubmed publisher
	Khoshnan A, Ko J, Patterson P. Effects of intracellular expression of anti-huntingtin antibodies of various specificities on mutant huntingtin aggregation and toxicity. Proc Natl Acad Sci U S A. 2002;99:1002-7 pubmed
	Ko J, Ou S, Patterson P. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Res Bull. 2001;56:319-29 pubmed

product information

Internal ID :

3999

Name :

MW7

Depositor Name :

Patterson, P.H.

Depositor Institution :

California Institute of Technology

Date Deposited :

1/23/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

huntingtin

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

mouse and human

Initial Publication Pubmed ID :

11719267

Collections :

Human,Neurodevelopment

Search Keywords :

Patterson, P.H., huntingtin, Human, MIgM, Human/Mouse, HTT, HD, IT15, AB_528296, monoclonal, Human/Neurodevelopment, Immunohistochemistry/Immunofluorescence/Western Blot/ELISA/Function Blocking

Antigen Molecular Weight :

Apparent: 348 kDa

Gene :

HTT

Alternate Gene Name(s) :

HD, IT15

Uniprot ID :

P42858

Antibody Registry ID :

AB_528296

Immunogen :

Recombinant Hd exon-1 67Q aggregate

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal, polyP

Epitope Map PubMed ID :

11719267

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Immunohistochemistry,Western Blot

Immunoblotting (legacy) :

Yes

Immunohistochemistry Pubmed IDs :

24798518 26436900

Immunofluorescence Pubmed IDs :

11719267 24798518 21209075 19491400 27221146 22037470

Western Blot Pubmed IDs :

11719267 19491400 22037470

ELISA Pubmed IDs :

11719267

Function Blocking Pubmed IDs :

19491400

Pubmed IDs :

11792860 21984825 28537272

Additional Information :

Western blot: in Human, weak binding to high molecular weight Htt, and strong binding to two smaller protein bands (doesn't differentiate between HD and non-HD); in mice, preferential strong binding to expanded polyQ domain. ICC: perinuclear or nuclear membranes in WT and expanded polyQ domain mutant; stains aggregates as inclusions in the nuclei of expanded polyQ domain mutant.

DSHB Growth Medium :

Iscove's

References (legacy) :

Brain Res. Bull. 56(3-4), 319-329.; Proc. Natl. Acad. Sci. USA 99(2), 1002-1007.

more info or order :

Developmental Studies Hybridoma Bank product webpage