huntingtin | Developmental Studies Hybridoma Bank MW3 product information

product summary

company name :

Developmental Studies Hybridoma Bank

product type :

antibody

product name :

huntingtin

catalog :

MW3

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

MW3

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

more info or order :

Developmental Studies Hybridoma Bank product webpage

citations: 2

Reference
Legleiter J, Lotz G, Miller J, Ko J, Ng C, Williams G, et al. Monoclonal antibodies recognize distinct conformational epitopes formed by polyglutamine in a mutant huntingtin fragment. J Biol Chem. 2009;284:21647-58 pubmed publisher
Ko J, Ou S, Patterson P. New anti-huntingtin monoclonal antibodies: implications for huntingtin conformation and its binding proteins. Brain Res Bull. 2001;56:319-29 pubmed

product information

Internal ID :

3975

Name :

MW3

Depositor Name :

Patterson, P.H.

Depositor Institution :

California Institute of Technology

Date Deposited :

1/23/03

Allow Hybridoma Distribution :

Yes

Cells Available (legacy) :

Yes

Antigen :

huntingtin

Antigen Species :

Human

Host Species :

mouse

Isotype :

MIgM

Isotype for catalog (legacy) :

IgM

Positive Tested Species Reactivity :

Human,Mouse

Species Tested (legacy) :

mouse and human

Initial Publication Pubmed ID :

11719267

Collections :

Human,Neurodevelopment

Search Keywords :

Patterson, P.H., huntingtin, Human, MIgM, Human/Mouse, HTT, HD, IT15, AB_528292, monoclonal, Human/Neurodevelopment, Immunofluorescence/Western Blot/ELISA/Function Blocking

Antigen Molecular Weight :

Apparent: 348 kDa

Gene :

HTT

Alternate Gene Name(s) :

HD, IT15

Uniprot ID :

P42858

Antibody Registry ID :

AB_528292

Immunogen :

Recombinant Hd exon-1 67Q/GST

Immunogen Sequence :

Partial protein

Clonality :

Monoclonal

Myeloma Strain :

HL-1

Epitope Mapped :

Yes

Epitope Location or Sequence :

N-terminal, polyQ

Epitope Map PubMed ID :

11719267

Recommended Applications :

ELISA,Function Blocking,Immunofluorescence,Western Blot

Immunoblotting (legacy) :

Yes

Immunofluorescence Pubmed IDs :

11719267 19491400

Western Blot Pubmed IDs :

11719267 19491400

ELISA Pubmed IDs :

11719267

Function Blocking Pubmed IDs :

19491400

Additional Information :

Western blot: preferentially binds expanded polyQ Htt, and smaller protein bands.

DSHB Growth Medium :

Iscove's

References (legacy) :

Brain Res. Bull. 56(3-4), 319-329.; Proc. Natl. Acad. Sci. USA 99(2), 1002-1007.

more info or order :

Developmental Studies Hybridoma Bank product webpage